We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV inserti...We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar,lower protease activity against azocasein,and lower activity for four glycosidases.This prtV mutant strain also had increased activity for two esterases in its extracellular products,as analyzed by the API ZYM system.In addition,the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes.Infection experiments showed that the LD50 of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish.We propose that prtV plays an important role in the pathogenesis of V.anguillarum.展开更多
Detecting the moving vehicles in jittering traffic scenes is a very difficult problem because of the complex environment.Only by the color features of the pixel or only by the texture features of image cannot establis...Detecting the moving vehicles in jittering traffic scenes is a very difficult problem because of the complex environment.Only by the color features of the pixel or only by the texture features of image cannot establish a suitable background model for the moving vehicles. In order to solve this problem, the Gaussian pyramid layered algorithm is proposed, combining with the advantages of the Codebook algorithm and the Local binary patterns(LBP) algorithm. Firstly, the image pyramid is established to eliminate the noises generated by the camera shake. Then, codebook model and LBP model are constructed on the low-resolution level and the high-resolution level of Gaussian pyramid, respectively. At last, the final test results are obtained through a set of operations according to the spatial relations of pixels. The experimental results show that this algorithm can not only eliminate the noises effectively, but also save the calculating time with high detection sensitivity and high detection accuracy.展开更多
The purpose of this paper is to introduce an innovative methodology in shipping investment evaluation based on simulation of a project cash flow. It concerns a complementary approach to current practices and its aim i...The purpose of this paper is to introduce an innovative methodology in shipping investment evaluation based on simulation of a project cash flow. It concerns a complementary approach to current practices and its aim is to provide more information mainly to ship-owners that consider the feasibility of deploying Ro-Ro Passenger ferries. The paper examines a typical semi-conventional RoPax ferry operating in the Aegean Sea. It addresses important economic, operational and procedural issues concerning risk evaluation. The current status is that risk is a notion still strange to main stakeholders. The Greek practice in performing a feasibility study for a project draws on investor＇s experience and feeling aided by a preliminary economic study. The main thesis of this paper is that there are alternative techniques to evaluate investment risk and measure profitability of a project. A Monte Carlo simulation in collaboration with a typical Ro-Ro cash-flow model is implemented to reveal the extent of risk and provide a useful tool for the assessment of future investments in Greek Short Sea Shipping.展开更多
The accumulation of heavy metals in the Avicennia marina was studied in the Bhira village mangrove forest, Miani Hor Balochistan, Pakistan. Samples of leaves, stem and roots were collected randomly from the selected s...The accumulation of heavy metals in the Avicennia marina was studied in the Bhira village mangrove forest, Miani Hor Balochistan, Pakistan. Samples of leaves, stem and roots were collected randomly from the selected specie （Avicennia marina）. The study site is receiving continuous amount of heavy metals from effluent and discharge of different sources. The concentrations of heavy metals was in decreasing order Fe 〉 Cd 〉 Pb 〉 Hg in all three parts （leaves, stem and roots） of the Avicennia marina. The average concentration of Fe was 6.45 μg/g, Cd 0.97μg/g, and Pb 0.71μg/g, in mangrove plant Avicennia marina. The situation reflects the needs for continuous monitoring of the heavy metals in the mangrove plant forest.展开更多
This paper studies the influence of radial depth on vibration, chip formation and surface roughness during face milling of AISl3O4 austenitic stainless steel with indexable cemented carbide milling cutters. The amplit...This paper studies the influence of radial depth on vibration, chip formation and surface roughness during face milling of AISl3O4 austenitic stainless steel with indexable cemented carbide milling cutters. The amplitude of vibration acceleration increased with the increasing radial depth up to 80 mm. And the domain vibration frequency varied with the radial depth. In this paper, three types of chips were found： C shape, long shape and spiral shape. The minimum surface roughness value occurred when the radial depth equalled 40 mm in the experiment. Irregular changes of chip curl radius and chip thickness could be attributed to different numbers of alternately engaged teeth when the feed and speed were fixed. Surface roughness is related to forced vibration and chip formation. Radial depth with different numbers of alternately engaged teeth could significantly influence the forced vibration, chip formation, and surface roughness.展开更多
Objective:To investigate gender difference in the effects of daytime sleep on item and source memories,which are dissociable elements of declarative memory,and the effects of sleep on recollection and familiarity,whic...Objective:To investigate gender difference in the effects of daytime sleep on item and source memories,which are dissociable elements of declarative memory,and the effects of sleep on recollection and familiarity,which are two processes underlying recognition. Methods:Participants saw a series of pictures with either blue or red background,and were then given a pretest for item and source memories. Then males and females respectively were randomly assigned either to a wake or a sleep condition. In the wake condition,participants remained awake until the posttest;in the sleep condition,participants slept for 1 h until awakened and asked to remain awake until the posttest. Results:Daytime sleep contributed to retention of source memory rather than item memory in females,whereas males undergoing daytime sleep had a trend towards increased familiarity. For females,however,neither recollection nor familiarity appeared to be influenced by daytime sleep. Conclusion:The mechanism underlying gender difference may be linked with different memory traces resulting from different encoding strategies,as well as with different electrophysiological changes during daytime sleep.展开更多
Strongly regular (α,β)-reguli are a class of incidence structures with given conditions which were introduced by Hamilton and Mathon. We introduce two classes of codes constructed from strongly regular (α,β)-regul...Strongly regular (α,β)-reguli are a class of incidence structures with given conditions which were introduced by Hamilton and Mathon. We introduce two classes of codes constructed from strongly regular (α,β)-reguli within PG(k-1,q). The codes are related with two-weight codes intimately.展开更多
Complexity measures for sequences, such as the linear complexity and the k-error linear complexity, play an important role in stream ciphers. This contribution studies the distribution of 1-error linear complexity of ...Complexity measures for sequences, such as the linear complexity and the k-error linear complexity, play an important role in stream ciphers. This contribution studies the distribution of 1-error linear complexity of binary sequences with arbitrary prime period. For any odd prime N, the authors present all possible values of 1-error linear complexity of N-periodic binary sequences, and derive the exact formulas to count the number of N-periodic binary sequences with any given 1-error linear complexity.展开更多
基金Supported by the National Basic Research Program of China (973 Program) (No. 2006CB101803)the National Natural Science Foundation of China (No. 30871935)the National High Technology Research and Development Program (863 Program) (No. 2003AA622070)
文摘We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar,lower protease activity against azocasein,and lower activity for four glycosidases.This prtV mutant strain also had increased activity for two esterases in its extracellular products,as analyzed by the API ZYM system.In addition,the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes.Infection experiments showed that the LD50 of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish.We propose that prtV plays an important role in the pathogenesis of V.anguillarum.
基金Project(61172047)supported by the National Natural Science Foundation of China
文摘Detecting the moving vehicles in jittering traffic scenes is a very difficult problem because of the complex environment.Only by the color features of the pixel or only by the texture features of image cannot establish a suitable background model for the moving vehicles. In order to solve this problem, the Gaussian pyramid layered algorithm is proposed, combining with the advantages of the Codebook algorithm and the Local binary patterns(LBP) algorithm. Firstly, the image pyramid is established to eliminate the noises generated by the camera shake. Then, codebook model and LBP model are constructed on the low-resolution level and the high-resolution level of Gaussian pyramid, respectively. At last, the final test results are obtained through a set of operations according to the spatial relations of pixels. The experimental results show that this algorithm can not only eliminate the noises effectively, but also save the calculating time with high detection sensitivity and high detection accuracy.
文摘The purpose of this paper is to introduce an innovative methodology in shipping investment evaluation based on simulation of a project cash flow. It concerns a complementary approach to current practices and its aim is to provide more information mainly to ship-owners that consider the feasibility of deploying Ro-Ro Passenger ferries. The paper examines a typical semi-conventional RoPax ferry operating in the Aegean Sea. It addresses important economic, operational and procedural issues concerning risk evaluation. The current status is that risk is a notion still strange to main stakeholders. The Greek practice in performing a feasibility study for a project draws on investor＇s experience and feeling aided by a preliminary economic study. The main thesis of this paper is that there are alternative techniques to evaluate investment risk and measure profitability of a project. A Monte Carlo simulation in collaboration with a typical Ro-Ro cash-flow model is implemented to reveal the extent of risk and provide a useful tool for the assessment of future investments in Greek Short Sea Shipping.
文摘The accumulation of heavy metals in the Avicennia marina was studied in the Bhira village mangrove forest, Miani Hor Balochistan, Pakistan. Samples of leaves, stem and roots were collected randomly from the selected specie （Avicennia marina）. The study site is receiving continuous amount of heavy metals from effluent and discharge of different sources. The concentrations of heavy metals was in decreasing order Fe 〉 Cd 〉 Pb 〉 Hg in all three parts （leaves, stem and roots） of the Avicennia marina. The average concentration of Fe was 6.45 μg/g, Cd 0.97μg/g, and Pb 0.71μg/g, in mangrove plant Avicennia marina. The situation reflects the needs for continuous monitoring of the heavy metals in the mangrove plant forest.
基金Supported by National Science and Technology Major Project of China(No.2009ZX04012-021)Major State Basic Research Development Program of China ("973" Program, No.2009CB724306)
文摘This paper studies the influence of radial depth on vibration, chip formation and surface roughness during face milling of AISl3O4 austenitic stainless steel with indexable cemented carbide milling cutters. The amplitude of vibration acceleration increased with the increasing radial depth up to 80 mm. And the domain vibration frequency varied with the radial depth. In this paper, three types of chips were found： C shape, long shape and spiral shape. The minimum surface roughness value occurred when the radial depth equalled 40 mm in the experiment. Irregular changes of chip curl radius and chip thickness could be attributed to different numbers of alternately engaged teeth when the feed and speed were fixed. Surface roughness is related to forced vibration and chip formation. Radial depth with different numbers of alternately engaged teeth could significantly influence the forced vibration, chip formation, and surface roughness.
基金supported partially by the National Basic Research Program (973) of China (No. 2006CB303101)the National Natural Science Foundation of China (No. 90820305)
文摘Objective:To investigate gender difference in the effects of daytime sleep on item and source memories,which are dissociable elements of declarative memory,and the effects of sleep on recollection and familiarity,which are two processes underlying recognition. Methods:Participants saw a series of pictures with either blue or red background,and were then given a pretest for item and source memories. Then males and females respectively were randomly assigned either to a wake or a sleep condition. In the wake condition,participants remained awake until the posttest;in the sleep condition,participants slept for 1 h until awakened and asked to remain awake until the posttest. Results:Daytime sleep contributed to retention of source memory rather than item memory in females,whereas males undergoing daytime sleep had a trend towards increased familiarity. For females,however,neither recollection nor familiarity appeared to be influenced by daytime sleep. Conclusion:The mechanism underlying gender difference may be linked with different memory traces resulting from different encoding strategies,as well as with different electrophysiological changes during daytime sleep.
基金the Scientific Research Start-up Foundation of Qingdao University of Science and Technology in China (No. 0022327)
文摘Strongly regular (α,β)-reguli are a class of incidence structures with given conditions which were introduced by Hamilton and Mathon. We introduce two classes of codes constructed from strongly regular (α,β)-reguli within PG(k-1,q). The codes are related with two-weight codes intimately.
基金supported by the National Natural Science Foundation of China under Grant Nos.61070178, 61100200,and 60833008
文摘Complexity measures for sequences, such as the linear complexity and the k-error linear complexity, play an important role in stream ciphers. This contribution studies the distribution of 1-error linear complexity of binary sequences with arbitrary prime period. For any odd prime N, the authors present all possible values of 1-error linear complexity of N-periodic binary sequences, and derive the exact formulas to count the number of N-periodic binary sequences with any given 1-error linear complexity.
