An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to identify ten species of Rhodiola, R. coccinea A. Bor, R. junggarica C.Y. Yang et N.R. Cui spn., R. heterodonta A. Bo...An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to identify ten species of Rhodiola, R. coccinea A. Bor, R. junggarica C.Y. Yang et N.R. Cui spn., R. heterodonta A. Bor, R. linearifolia A. Bor, R. pamiro alaiucm A. Bor, R. kaschgarica A. Bor, R. litwinowii A. Bor, R. gelida schrenk, R. rosea L. and R. quadrifide Fisch et Mey collected from the Tianshan Mountains areas of China. Chromatograms of alcohol-soluble proteins, generated from these ten Rhodiola spp. were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild species only. The results showed that when using a Waters Delta Pak. C18, 5 μm particle size reversed phase column (150 mm×3.9 mm), a linear gradient of 22%?55% solvent B with a flow rate of 1 ml/min and a run time of 67 min, the chromatography gave optimum separation of Rhodiola alcohol-soluble proteins. Chromatogram of each species was different and could be used to identify those species. Cluster analysis of genetic similarity coefficients of 37% to 60% showed a medium degree of genetic diversity among the species in these eco-areas. Cluster analysis showed that the ten species of Rhodiola can be divided into four clusters and yielded the general and unique biochemical markers of these species. RP-HPLC was shown to be a rapid, repeatable and reliable method for Rhodiola species identification and analysis of genetic diversity.展开更多
Drought tolerant analysis using carbon isotopes discrimination could be useful tool to decide a genotype with high adaptation to drought. A pot culture experiment was conducted in glass house at the IAEA （Internation...Drought tolerant analysis using carbon isotopes discrimination could be useful tool to decide a genotype with high adaptation to drought. A pot culture experiment was conducted in glass house at the IAEA （International Atomic Energy Agency） laboratories, Seibersdorf, Austria to analyze drought tolerant of wheat genotype using carbon isotopes discrimination technique. Four wheat genotypes viz., LU-26s, Bhittai, Roshan, Taifu, were tested. There were two treatments： Control （normal irrigation） and Drought （@ 30% field capacity of the control treatment）. Drought treatment was imposed after two weeks of germination. The experiment was continued up to 10 weeks after germination and was terminated at flowering stage. The data were recorded in terms of plant height, number of tillers, shoot dry weight and carbon isotopic ratio （δ）. Results showed that genotype LU-26s was found to have best performance under drought condition, with minimum decrease in the growth parameters i.e., plant height, number of tillers and shoot dry weight. High CID （carbon isotopes discrimination） values were also observed in genotype LU-26s, showing close positive correlation between SDW （shoot dry weight） and CID. It is therefore concluded that carbon isotopes discrimination can be an important criterion for the selection of wheat genotypes for drought prone areas.展开更多
基金Project (No. 30470330) supported by the National Natural ScienceFoundation of China
文摘An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to identify ten species of Rhodiola, R. coccinea A. Bor, R. junggarica C.Y. Yang et N.R. Cui spn., R. heterodonta A. Bor, R. linearifolia A. Bor, R. pamiro alaiucm A. Bor, R. kaschgarica A. Bor, R. litwinowii A. Bor, R. gelida schrenk, R. rosea L. and R. quadrifide Fisch et Mey collected from the Tianshan Mountains areas of China. Chromatograms of alcohol-soluble proteins, generated from these ten Rhodiola spp. were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild species only. The results showed that when using a Waters Delta Pak. C18, 5 μm particle size reversed phase column (150 mm×3.9 mm), a linear gradient of 22%?55% solvent B with a flow rate of 1 ml/min and a run time of 67 min, the chromatography gave optimum separation of Rhodiola alcohol-soluble proteins. Chromatogram of each species was different and could be used to identify those species. Cluster analysis of genetic similarity coefficients of 37% to 60% showed a medium degree of genetic diversity among the species in these eco-areas. Cluster analysis showed that the ten species of Rhodiola can be divided into four clusters and yielded the general and unique biochemical markers of these species. RP-HPLC was shown to be a rapid, repeatable and reliable method for Rhodiola species identification and analysis of genetic diversity.
文摘Drought tolerant analysis using carbon isotopes discrimination could be useful tool to decide a genotype with high adaptation to drought. A pot culture experiment was conducted in glass house at the IAEA （International Atomic Energy Agency） laboratories, Seibersdorf, Austria to analyze drought tolerant of wheat genotype using carbon isotopes discrimination technique. Four wheat genotypes viz., LU-26s, Bhittai, Roshan, Taifu, were tested. There were two treatments： Control （normal irrigation） and Drought （@ 30% field capacity of the control treatment）. Drought treatment was imposed after two weeks of germination. The experiment was continued up to 10 weeks after germination and was terminated at flowering stage. The data were recorded in terms of plant height, number of tillers, shoot dry weight and carbon isotopic ratio （δ）. Results showed that genotype LU-26s was found to have best performance under drought condition, with minimum decrease in the growth parameters i.e., plant height, number of tillers and shoot dry weight. High CID （carbon isotopes discrimination） values were also observed in genotype LU-26s, showing close positive correlation between SDW （shoot dry weight） and CID. It is therefore concluded that carbon isotopes discrimination can be an important criterion for the selection of wheat genotypes for drought prone areas.
