AIM: To investigate whether serum levels of two soluble forms of extracellular cytokeratin 18 (M30-antigen and M65-antigen) may differentiate nonalcoholic steatohepatitis (NASH) from simple steatosis in patients with ...AIM: To investigate whether serum levels of two soluble forms of extracellular cytokeratin 18 (M30-antigen and M65-antigen) may differentiate nonalcoholic steatohepatitis (NASH) from simple steatosis in patients with nonalcoholic fatty liver disease (NAFLD). METHODS: A total of 83 patients with suspected NAFLD and 49 healthy volunteers were investigated. Patients with suspected NAFLD were classified according to their liver histology into four groups: definitive NASH (n = 45), borderline NASH (n = 24), simple fatty liver (n = 9), and normal tissue (n = 5). Serum levels of caspase-3 generated cytokeratin-18 fragments (M30-antigen) and total cytokeratin-18 (M65-antigen) were determined by ELISA. RESULTS: Levels of M30-antigen and M65-antigen were significantly higher in patients with definitive NASH compared to the other groups. An abnormal value (> 121.60 IU/L) of M30-antigen yielded a 60.0% sensitivity and a 97.4% specificity for the diagnosis of NASH. Sensitivity and specificity of an abnormal M65-antigen level (> 243.82 IU/L) for the diagnosis of NASH were 68.9% and 81.6%, respectively. Among patients with NAFLD, M30-antigen and M65-antigen levels distinguished between advanced fibrosis and early-stage fibrosis with a sensitivity of 64.7% and 70.6%, and a specificity of 77.3% and 71.2%, respectively. CONCLUSION: Serum levels of M30-antigen and M65-antigen may be of clinical usefulness to identify patients with NASH. Further studies are mandatory to better assess the role of these apoptonecrotic biomarkers in NAFLD pathophysiology.展开更多
A direct linear discriminant analysis algorithm based on economic singular value decomposition （DLDA/ESVD） is proposed to address the computationally complex problem of the conventional DLDA algorithm, which directl...A direct linear discriminant analysis algorithm based on economic singular value decomposition （DLDA/ESVD） is proposed to address the computationally complex problem of the conventional DLDA algorithm, which directly uses ESVD to reduce dimension and extract eigenvectors corresponding to nonzero eigenvalues. Then a DLDA algorithm based on column pivoting orthogonal triangular （QR） decomposition and ESVD （DLDA/QR-ESVD） is proposed to improve the performance of the DLDA/ESVD algorithm by processing a high-dimensional low rank matrix, which uses column pivoting QR decomposition to reduce dimension and ESVD to extract eigenvectors corresponding to nonzero eigenvalues. The experimental results on ORL, FERET and YALE face databases show that the proposed two algorithms can achieve almost the same performance and outperform the conventional DLDA algorithm in terms of computational complexity and training time. In addition, the experimental results on random data matrices show that the DLDA/QR-ESVD algorithm achieves better performance than the DLDA/ESVD algorithm by processing high-dimensional low rank matrices.展开更多
文摘AIM: To investigate whether serum levels of two soluble forms of extracellular cytokeratin 18 (M30-antigen and M65-antigen) may differentiate nonalcoholic steatohepatitis (NASH) from simple steatosis in patients with nonalcoholic fatty liver disease (NAFLD). METHODS: A total of 83 patients with suspected NAFLD and 49 healthy volunteers were investigated. Patients with suspected NAFLD were classified according to their liver histology into four groups: definitive NASH (n = 45), borderline NASH (n = 24), simple fatty liver (n = 9), and normal tissue (n = 5). Serum levels of caspase-3 generated cytokeratin-18 fragments (M30-antigen) and total cytokeratin-18 (M65-antigen) were determined by ELISA. RESULTS: Levels of M30-antigen and M65-antigen were significantly higher in patients with definitive NASH compared to the other groups. An abnormal value (> 121.60 IU/L) of M30-antigen yielded a 60.0% sensitivity and a 97.4% specificity for the diagnosis of NASH. Sensitivity and specificity of an abnormal M65-antigen level (> 243.82 IU/L) for the diagnosis of NASH were 68.9% and 81.6%, respectively. Among patients with NAFLD, M30-antigen and M65-antigen levels distinguished between advanced fibrosis and early-stage fibrosis with a sensitivity of 64.7% and 70.6%, and a specificity of 77.3% and 71.2%, respectively. CONCLUSION: Serum levels of M30-antigen and M65-antigen may be of clinical usefulness to identify patients with NASH. Further studies are mandatory to better assess the role of these apoptonecrotic biomarkers in NAFLD pathophysiology.
基金The National Natural Science Foundation of China (No.61374194)
文摘A direct linear discriminant analysis algorithm based on economic singular value decomposition （DLDA/ESVD） is proposed to address the computationally complex problem of the conventional DLDA algorithm, which directly uses ESVD to reduce dimension and extract eigenvectors corresponding to nonzero eigenvalues. Then a DLDA algorithm based on column pivoting orthogonal triangular （QR） decomposition and ESVD （DLDA/QR-ESVD） is proposed to improve the performance of the DLDA/ESVD algorithm by processing a high-dimensional low rank matrix, which uses column pivoting QR decomposition to reduce dimension and ESVD to extract eigenvectors corresponding to nonzero eigenvalues. The experimental results on ORL, FERET and YALE face databases show that the proposed two algorithms can achieve almost the same performance and outperform the conventional DLDA algorithm in terms of computational complexity and training time. In addition, the experimental results on random data matrices show that the DLDA/QR-ESVD algorithm achieves better performance than the DLDA/ESVD algorithm by processing high-dimensional low rank matrices.
