通过对稻田黑土区土壤昆虫的种类与数量进行采样调查,共记录了稻田黑土区土壤昆虫5目14科20属26种,土壤昆虫优势种共14个,个体数量最多的是粘虫(Leucania separate Walker)和红脊长蝽(Tropidothorax elegana Distant),本研究将为改良农...通过对稻田黑土区土壤昆虫的种类与数量进行采样调查,共记录了稻田黑土区土壤昆虫5目14科20属26种,土壤昆虫优势种共14个,个体数量最多的是粘虫(Leucania separate Walker)和红脊长蝽(Tropidothorax elegana Distant),本研究将为改良农业生产环境和完善农业生产方式提供科学依据,为科学管理稻田、提高农作物的产品质量提供直接或间接的帮助。展开更多
The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, se...The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.展开更多
DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar specie...DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.展开更多
文摘通过对稻田黑土区土壤昆虫的种类与数量进行采样调查,共记录了稻田黑土区土壤昆虫5目14科20属26种,土壤昆虫优势种共14个,个体数量最多的是粘虫(Leucania separate Walker)和红脊长蝽(Tropidothorax elegana Distant),本研究将为改良农业生产环境和完善农业生产方式提供科学依据,为科学管理稻田、提高农作物的产品质量提供直接或间接的帮助。
基金Project(50925417)supported by the National Funds for Distinguished Young Scientist,ChinaProject(2012BAC09B04)supported by the National Key Technology Research and Development Program,China+2 种基金Project supported by the Post-doctoral Program of Central South University,ChinaProjects(31100082,61171061)supported by the National Natural Science Foundation of ChinaProject(2012SK4028)supported by the Science and Technology Program of Hunan Province,China
文摘The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest (No. 201003068)Special Key Program of Zhejiang Provincial Department of Science and Technology (No. 2008C12011)
文摘DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.
