韩国职业培训与职业技能鉴定制度

韩国职业培训与职业技能鉴定制度□张永麟韩国自然资源贫乏，但在短短的十几年韩国经济高速发展，成为亚洲“四小龙”，韩国发展的一条成功经验是非常重视职业培训，有完善的法律法规体系，完备的职业培训、职业技能鉴定和职业技能竞赛制度。一、建立完善的职业培训法律法...

工程设计实行鉴定制的几点启示

对佳木斯铁路分局以往在设计文件审批工作中所采用习惯做法的弊端进行了剖析，提出设计鉴定制的审批方法，详细阐述了该方法的工作程序及应用所取得的良好效果，并对如何进一步做好设计文件鉴定提出了改进措施。

2018-102015-9 H012三个茶树新品系的适制性鉴定及其香气分析

以福鼎大白茶(CK)、黔湄809(CK)、2018-10、2015-9、H012一芽二三叶为原料,分别将其制成红茶和绿茶,通过感官审评、儿茶素含量与香气成分对比分析,探讨3个茶树新品系的适制性和香气特征。结果表明,绿茶感官品质综合得分表现为H012(93.80分)>2018-10(92.15分)>福鼎大白(92.05分)>2015-9(90.70分),红茶感官品质综合得分表现为2018-10(93.00分)>2015-9(92.95分)>黔湄809(92.00分)。H012适制绿茶,其主要香气成分为醇类(46.55%)、酯类(14.69%)、醛类(10.79%)、酮类(9.27%)、碳氢类(8.89%);2018-10适制绿茶和红茶,其绿茶主要香气成分为醇类(26.31%)、碳氢类(21.62%)、醛类(16.40%)、酯类(12.05%)、含氮类(10.81%)、酮类(6.50%)、其他类(1.71%)、酚类(1.51%);2015-9适制红茶,其主要香气成分为醇类(46.78%)、醛类(26.82%)、酯类(8.34%)、碳氢类(8.25%)、酮类(4.87%)、其他类(3.80%)。

试谈中小学骨干教师学科带头人的激励与培养

在中小学骨干教师"学科带头人"的培养中,应坚持以鉴定制取代选拔制,以流动制取代终身制,以培养个体达到促进发展全员的目的。影响"学科带头人"积极性的因素是多方面的,要激励"学科带头人"的积极性,应该加强思想教育工作,提高"学科带头人"的思想境界,协调"学科带头人"的人际关系,满足"学科带头人"的物质和精神需要,明确"学科带头人"的职责和权利。

Preparation and Preliminary Identification of Fluorescein Labeled Monoclonal Antibody against Canine Distemper Virus

[Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper （CD） with FITC-conjugated monoclonal antibodies （FITC-McAb）.[ Metbod] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofluorescence assay. [ Result] The absorption test, blocking test and specificity test showed that the labeled antibody had high specificity and sensitivity, but didn＇t have cross reaction with canine parvovirus （CPV）, canine parainfluenza virus （CPIV）, canine adenovirus （CAV） and rabies virus （RV）. The optimal working concentration was 1：80. The positive rate of clinical suspected samples was 48%. [ Conclusion] The direct immunofluorescence assay developed in this study was rapid, specific and convenient, and had great significance for the early diagnosis of canine distemper.

新疆褐牛体尺体重相关性及主成分分析

在建立新疆褐牛9分制的鉴定标准过程中,通过对伊犁地区新源县、尼勒克县、昭苏县3个牛场中的569头新疆褐牛成年母牛进行测量,选取13个体重体尺性状进行相关性及主成分分析。结果表明:新疆褐牛体重与体高、体斜长、胸围、腹围、尻宽、尻长、后腿宽度、后腿厚度、后腿半围等具有极显著相关性(P<0.01);13个主成分中第1主成分的贡献率最大,反映了新疆褐牛的外貌整体结构信息,第2和第3主成分反映了后躯特征和尻部特征。

医疗诉讼的特点分析

本文通过对我国当前的医疗诉讼自身的特点进行分析,总结出目前的医疗诉讼在制度上存在以下问题:第一,多层次的法律规范之间存在矛盾和冲突;第二,举证责任倒置存在有待完善的方面;第三,医疗证据鉴定的双轨制度带来了一系列负面影响。

Extraction of Polysaccharides from Spirulina with Boiling Water

[Objective] The aim of this study was to develop a simple and effective method for the polysaccharide extraction from Spirulina. [Method] The polysaccha- rides were directly extracted from the fresh algae mat or dry powder of Spirulina in boiling water. The contents and quality of the extracted polysaccharides were measured. [Result] On average, 236.06 g of polysaccharide was extracted from 25 kg of fresh Spirulina mat, with a yield rate of 0.94%, while 191.95 g of polysac- charide was extracted from 2.5 kg of dry powder of Spirulina with a yield rate of 0.77%. The polysaccharide content in the extract of fresh Spirulina mat was 12.56% （according to glucose content）, while that of the dry powder was 12.38% （according to glucose content）; the glucose was produced during the hydrolysis of Spirulina polysaccharide. [Conclusion] Extraction polysaccharide from Spirulina with boiling wa- ter greatly reduces the use of ethanol, and the possibility of pollution from the ex- ogenous non-food chemical reagents, so that the extracted polysaccharides can be used as food materials. This method makes it possible to establish the production line for Spirulina polysaccharide.

Association betweenITGA2 C807T polymorphism and gastric cancer risk

AIM： To evaluate the impact of the ITGA2 gene polymorphism on gastric cancer risk. METHODS： A hospital-based case-control study was conducted, including 307 gastric cancer patients and 307 age- and gender-matched control subjects. The genotypes were identified by polymerase chain reaction-restriction fragment length polymorphism assay. RESULTS： The frequencies of the wild and variant genotypes in cases were significantly different from those of controls （P = 0.019）. Compared with individuals with the wild genotype CC, subjects with the variant genotypes （CT ＋ IT） had a significantly higher risk of gastric cancer （adjusted odds ratio = 1.57, 95% CI = 1.13-2.17, P = 0.007）. In stratified analyses, the elevated gastric cancer risk was especially evident in older individuals aged 〉 58 years, nonsmokers and rural subjects. Further analyses revealed that the variant genotypes were associated with poor tumor differentiation and adjacent organ invasion in the sub-analysis of gastric cancer patients. CONCLUSION： The ITGA2 gene C807T polymorphism may be associated with an increased risk of gastric cancer, differentiation and invasion of gastric cancer.

Identification of up-regulated genes in human uterine leiomyoma by sup-pression subtractive hybridization

In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes werefirstly associated with UL. Three genes with notable difference were selected for Northern confirmationOur results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showedup-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obviousexpression in prostate, testis, liver, heart and skeletal muscle.

Isolation and characterization of bioactive fungi from shark Carcharodon carcharias' gill with biopharmaceutical prospects

Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark （Carcharodon carcharias）. A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit （ITS-LSU） rDNA-sequence BLAST comparison. Four genera, Penicillium, Aspergillus, Mucor, and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fimgi found in a shark＇s gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the first study characterizing shark gill fungi and their bioactivity.

Identification of a Putative Tetrasporophyte-Specific Gene in Gracilaria lemaneiformis(Gracilariales,Rhodophyte)

A putative tetrasporophyte-specific gene,designated as SSH466(GenBank accession No. DQ019223) ,was one of the genes identified in this work using suppression subtractive hybridization(SSH) method in Gracilaria lemaneiformis. The full length of the gene was obtained using SMART RACE strategy. Sequence analysis revealed that the gene had 1 019 nucleotides,including an open reading frame of 498 nucleotides encoding 166 amino acid residues,158 nucleotides of 5' untranslated region and 363 nucleotides of 3' non-coding region. Protein motif and secondary structure prediction showed that there existed a transmembrane domain with a unique β-sheet. Thus,SSH466 protein might be a cross-membrane protein. Sequence homology search in the public GenBank databases did not reveal any significant match with SSH466. Virtual Northern blot analysis confirmed that it was a tetrasporophyte-specific gene.

Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials

Objective： To identify differentially expressed long non-coding RNAs （lncRNAs） involved in the metastasis of epithelial ovarian cancer. Methods： An in vitro invasion assay was performed to validate the invasive capability of SKOV3 and SKOV3.ip1 cell lines. Total R.NA was then extracted, and microarray analysis was performed. Moreover, nine lncRNAs were selected for validation using RT-qPCR. Results： Compared with the SKOV3 cells, the SKOV3.ip1 cells significantly improved in the in vitro invasive activity. Of the 4,956 lncRNAs detected in the microarra~ 583 and 578 lncRNAs were upregulated and downregulated, respectivel~ in SKOV3.ip1 cells, compared with the parental SKOV3 cells. Seven of the analyzed lncRNAs （MALAT1, H19, UCA1, CCAT1, LOC645249, LOC100128881, and LOC100292680） confirmed the deregulation found by microarray analysis. Conclusion： LncRNAs clusters were differentially expressed in ovarian cancer cells with varying metastatic potentials. This result indicates that some lncRNAs might exert a partial or key role in epithelial ovarian cancer metastasis. Further studies should be conducted to determine the roles of these lncRNAs in ovarian cancer metastasis.

Levitation mechanism modelling for maglev transportation system

A novel maglev transportation system was proposed for large travel range ultra precision motion.The system consists of a levitation subsystem and a propulsion subsystem.During the propulsion subsystem driving the moving platform along the guideway,the levitation subsystem uses six pairs of electromagnets to steadily suspend the moving platform over the guideway.The model of the levitation system,which is a typical nonlinear multi-input multi-output coupling system and has many inner nonlinear coupling characteristics,was deduced.For testifying the model,the levitation mechanism was firstly controlled by proportional-integral-differential(PID) control,and then a lot of input-output data were collected for model parameter identification.The least-square parameter identification method was used.The identification results prove that the model is feasible and suitable for the real system.

Identification of Five Sea Cucumber Species Through PCR-RFLP Analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene(COI) was used to identifing five sea cucumber species(Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31 I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.

Research on Secure Authentication Mechanism of Grid

Based on the analysis on the security mechanism of grid, this paper discusses the advantages of latest techniques of grid security, and presents a secure authentication scheme of grid based on elliptic curve cryptosystem (ECC). The secure authentication scheme may provide reference value for the further application of grid.

Identification of Stochastic Nature of Yarn Hairiness

Yam hairiness is a complex concept, which generally cannot be completely defined by a single figure. Hairiness can be considered as the fiber ends and loops standing out from the main compact yarn body. Uster hairiness system characterizes the hairiness by H value, i.e. the mean value of the total length of all hairs within one centimeter of yarn. The raw data HI are in fact realization of spatial process （hairiness spatial process -- HSP） and can be used for more complex evaluation of hairiness characteristics in the space and frequency domain. The main aim of this contribution is description of some tools for spatial characterization of yarn hairiness. The simple methods for complex characterization of lISP statistical behavior （stationarity, independence, linearity etc. ） are presented. The techniques based on the embedding dimension and correlation integral or long-range dependences evaluation are discussed. The selected methods are core of HYARN program in MATLAB. Application of this program for deeper characterization of artificial data and cotton type yam are shown.

Design of Calibration Experiments for Identification of Manipulator Elastostatic Parameters

The paper is devoted to the elastostatic calibration of industrial robots, which is used for precise machining of large-dimensional parts made of composite materials. In this technological process, the interaction between the robot and the workpiece causes essential elastic deflections of the manipulator components that should be compensated by the robot controller using relevant elastostatic model of this mechanism. To estimate parameters of this model, an advanced calibration technique is applied that is based on the non-linear experiment design theory, which is adopted for this particular application. In contrast to previous works, it is proposed a concept of the user-defined test-pose, which is used to evaluate the calibration experiments quality. In the frame of this concept, the related optimization problem is defined and numerical routines are developed, which allow generating optimal set of manipulator configurations and corresponding forces/torques for a given number of the calibration experiments. Some specific kinematic constraints are also taken into account, which insure feasibility of calibration experiments for the obtained configurations and allow avoiding collision between the robotic manipulator and the measurement equipment. The efficiency of the developed technique is illustrated by an application example that deals with elastostatic calibration of the serial manipulator used for robot-based machining.