针灸治疗耳鸣的现状及文献质量评价

目的利用数据挖掘技术探究近30年来针灸治疗耳鸣的临床文献,进行相关性分析。方法检索维普数据网(VIP)、中国知网(CNKI)1989~2018年间涉及针灸治疗耳鸣的临床文献,筛选纳入符合标准的临床文献采用Microsoft Excel、SPSS Clementine软件中Apriori算法等进行信息挖掘。结果经统计发现纳入的175篇文献中,虽然结果显示针灸治疗耳鸣的主穴主要为听会、翳风、听宫,但是只有54.15%的支持度;Jadad分析统计高质量文献仅有13篇(7.43%);有明确诊断标准的文献103篇(58.86%);明确提出疗效判定标准的文献63篇(36%);随机对照文献117篇(66.86%)。结论近30年来针灸治疗耳鸣的取穴、组方有一定规律可循,但由于高质量文献数量较少,不能完全客观、透明的反映临床研究,所以只能一定程度上说明针灸治疗耳鸣采用听会、翳风、听宫较多。故临床需要设计更为严谨的试验方案,为今后临床治疗与试验研究提供科学指导。

Enhanced expression of epidermal growth factor receptor gene in gastric mucosal cells by the serum derived from rats treated with electroacupuncture at stomach meridian acupoints

AIM: To investigate the effect of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expression of epidermal growth factor receptor (EGFR) gene in gastric mucosal cells. METHODS: The stress-induced gastric mucosal injury in rat model was established by water-immersion and restrained stress methods. 52 rats were randomly divided into: normal group (n = 8), model group (n = 8), model serum group (n = 12), stomach serum group (n = 12), and gallbladder serum group (n = 12). The gastric mucosal cells were separated by pronase-EDTA digestion method and incubated with serum. The EGFR gene expression in gastric mucosal cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: Compared with normal group (0.6860 ± 0.0594), the serum derived from rats of the stomach group (1.2272 ± 0.0813, P = 0.00 < 0.01) and gallbladder group (0.9640 ± 0.0387, P = 0.00 < 0.01) had a tendency to enhance the EGFR gene expression in gastric mucosal cells. Such tendency existed in the model group (0.7104 ± 0.0457) but with no signifi cant difference (P = 0.495 > 0.05) and in model serum group (0.8516 ± 0.0409) with an extremely obvious difference (P = 0.001 < 0.01). Furthermore, the EGFR gene expression in stomach serum group was significantly higher than that in gallbladder serum group (P = 0.00 < 0.01). CONCLUSION: The present study shows that serumderived from rats treated with electroacupuncture at stomach meridian acupoints can distinctly increase the EGFR gene expression of gastric mucosal cells. Therefore, there is certain meridian specificity in the serum, which could provide a proof for the TCM theory “particular relation between meridian and internal organ”.

Regulative Effects of Auricular Acupuncture,Moxibustion and Chinese Herbs on Immunologic Function in the D-Galactose-induced Aging Mouse

Objective:To observe the regulative effect of auricular acupuncture, moxibustion and Chinese herbs on immunologic function in the D-galactose-induced aging mouse and to probe the feasibility and possible mechanisms for delaying aging, so as to provide experimental basis for acupuncture, moxibustion and Chinese herbs for delaying aging. Methods: Aging mouse model was established by subcutaneous injection of D-galactose, and treated by auricular acupuncture, auricular acupuncture plus moxibustion, and auricular acupuncture plus Chinese herbs respectively for 6 weeks and then serum interleukin-2 (IL-2) and IL-6 contents and splenic lymphocyte transformation rate were compared among the groups. Results: Compared with the young group, the serum IL-2 level and the splenic lymphocyte transformation rate significantly decreased and the IL-6 level significantly increased in the aging mouse. After treatment by auricular acupuncture, auricular acupuncture plus Chinese herbs, especially auricular acupuncture plus moxibustion, the serum IL-2 level and the splenic lymphocyte transformation rate significantly increased, and the IL-6 level significantly decreased as compared with the model group. Conclusions: Combined application of auricular acupuncture, moxibustion and Chinese herbs can improve the decline or dysfunction of immunological function in the aging organism to a certain extent.

80 CASES OF PERIPHERAL FACIAL PARALYSIS TREATED BY ACUPUNCTURE WITH VIBRATING SHALLOW INSERTION

Eighty cases of peripheral facial paralysis were treated by acupuncture with vibrating shallow insertion. The total effective rate was 98.75% and cure rate 88.75%. There was no significant difference in therapeutic effect as compared with the conventional electroacupuncture method (P > 0.5), suggesting that shallow puncture by vibratings can also yield satisfactory therapeutic results.

Gene control of acupuncture and moxibustion preconditioning on apoptosis in ischemic cardiac muscle of rats with re-perfusion

In order to explore the effect of acupuncture preconditioning on rats＇ cell apoptosis with cardiac muscle re-perfusion damage and bcl-2mRNA genes, we used differentiating acupuncture and moxibustion preconditioning among groups, then compared acupuncture and moxibustion preconditioning with ischemic preconditioning. The experimental results show that acupuncture and moxibustion preconditioning makes more bcl-2mRNA genes expressed and produces less cell apoptosis, furthermore, groups of acupuncture and moxibustion preconditioning for twice a day are more effective than those of ischemic ureconditioning.

THE ROLE OF CONNEXIN 43 GENE IN ACUPUNCTURE ANALGESIA

Objective To and connexin 43. Methods investigate the possible relationship between the analgesic effect of acupuncture Connexin 43 gene knock-out mice were randomly divided into 4 groups： a wide type （WT） control group, a WT acupuncture group, a heterozygous （HT） control group and HT acupuncture group. Hot-plate test and writhing response induced by acetic acid were used for investigating the different analgesic effect of acupuncture on HT and WT mice. Results There was no significant difference in the basic pain threshold value between HT and WT mice （P 〉0.05）. Acupuncture could significantly increase the pain threshold value, prolong the latency period of writhing body and decrease the number of writhing body as compared with pre-acupuncture in WT and HT mice （P 〈 0.01 or P 〈 0.05）. The pain threshold, latency period of writhing and number of writhing body in HT mice were less than WT mice post-acupuncture （P〈0.05）. Conclusion Connexin 43 gene knock-out might partially inhibit the analgesic effect of acupuncture, suggesting that connexin 43 is possibly related with meridians and the effect of acupuncture.

OBSERVATION ON THE THERAPEUTIC EFFECT OF BIRD-PECKING MOXIBUSTION OF SPECIFIC ACUPOINTS IN THE TREATMENT OF FUNCTIONAL DYSPEPSIA

Objective: To observe the therapeutic effect of moxibustion of some specific acupoints for treatment of functional dyspepsia (FD, qi-distension type). Methods:A total of 50 cases of FD patients were randomly divided into treatment group (n=26) and medication group (n=24). In treatment group, bird-pecking moxibustion was applied to Zhongwan (CV 12), Qihai (CV 6), Neiguan (PC 6) and Gongsun (SP 4) 5～6 times in every treatment till the local skin got flush. The treatment was given once daily, continuously for 10 days. In control group, the patients were ordered to take Motilium 10 mg/time, 3 times daily. Following 10 days’ treatment the therapeutic effect was assessed.Results:After treatment, of the 26 cases in treatment group, 11 (42.3%) were cured, 7 (26.92%) had remarkable improvement in their symptoms, 6 (23.07%) had improvement and 2 (7.69%) failed, with a total effective rate of 92.31%. Of the 24 cases in medication group, 9 (37.50%) were cured, 6 (25.00%) experienced remarkable improvement in their symptoms, 6 (25.00%) were improved and 3 (12.50%) failed, with a total effective rate of 87.50%. Comparison between two groups showed that the therapeutic effect of moxibustion was significantly superior to that of medication (P<0.05). In addition, the difference value of the therapeutic effect index (TEI) between post-and pre-treatment in treatment group was also significantly higher than that of medication group (P<0.05). Conclusion: Bird-pecking moxibustion of specific acupoints can effectively improve clinical symptoms of FD patients.

EVIDENCE-BASED MEDICINE AND THE CLINICAL STUDY ON ACUPUNCTURE AND MOXIBUSTION

In this article, the authors hold that the evidence based medicine(EBM) is a new medical action produced at the historic moment in clinical practice, which is promoting development of the medicine and even the whole life science in a fully new train of thought and method. Further the briefly introduced contents are the effect of EBM, the difficulty of utilizing in the acupuncture clinical practice and how to resolve them. It is highly recommended that the doctors of the acupuncture science field should study EBM and its effect and difficulties in practice as earlier as possible, insistently assimilate new knowledge and keep abreast of the times’ progress to facilitate the further development of acupunctology.

TREATMENT OF SOFT TISSUE INJURY BY PUNCTURING CLEFT-POINTS

Objective: To observe the therapeutic effect of acupuncture of "Xi" (Cleft) points in treatment of soft tissue injury. Methods: 335 cases of soft tissue injury patients were divided into Cleft point group (264 cases) and Ashi point group (control group, 71 cases) randomly. In Cleft point group, the 16 Cleft points were used in combination with Ahshi points. In control group, only local Ahshi points were punctured. The treatment was conducted once every day, with 5 sessions being a therapeutic course. After 2 courses of treatment, the therapeutic effect was analyzed. Results: Results showed that the therapeutic effect of cleft point group was significantly better than that of control group (P<0.05), particularly in treatment of acute soft tissue. Conclusion: Cleft point acupuncture has a better therapeutic effect in treatment of soft tissue injury in comparison with Ashi point.

APPLICATION OF PRINCIPLE OF "PREVENTION IN PREFERENCE TO TREATMENT OF DISEASES" IN ACUPUNCTURE-MOXIBUSTION CLINIC

In the present paper the author reviews the viewpoint of 'preventive treatment of diseases' in the ancient literature of traditional Chinese medicine (TCN) and its clinical application from (1) prevention first before the occurrence of diseases; and (2) preventing development after onset of diseases. In the preventive treatment of diseases, the ancient Chinese doctors usually (1) regulated qi of both Conception Vessel and Governor Vessel for health care; (2) performed regular moxibustion; and (3) applied plaster to the acupoint in summer for treating winter-diseases. In the treatment of diseases after onset, the ancient Chinese usually (1) tried best to make early diagnosis and early treatment; and (2) strengthened the related internal organ in advance to check their development; and (3) employed appropriate remedies to recuperate the patient's health.

EFFECTS OF ACUPUNCTURE ON THE PITUITARYTHYROID AXIS IN RABBITS WITH FRACTURE

PURPOSE: To observe the effects of acupuncture on the pituitary-thyroid axis in rabbits with fracture. METHOD: 36 male rabbits with a 3-mm width experimental defect in the middle segment of the left radius were randomly divided into the treatment group and control group, and the changes in serum T3, T4 and TSH contents in different weeks were investigated. RESULTS: Serum T3, T4 and TSH contents in the treatment group all were higher than those in the control group, with statistically significant differences in the contents of T4 and TSH in the second week and T3 content in the fourth week (P

CLINICAL OBSERVATION ON TREATMENT OF ACUTE BRONCHITIS PRIMARILY WITH PRICKING-CUPPING ON BACK-SHU POINTS

To observe the clinical effect of treatment of acute bronchitis primarily with prickingcupping method on Back-shu points. Methods. The patients of acute bronchitis were randomly divided into 2 groups. In the observation group, there were 36 cases, who were treated with the integrated traditional Chinese and western medicine, primarily the pricking-cupping method on Back-shu points; while in the control group, there were 29 cases who were given the conventinal treatment of western medicine. All the 2 groups were treated for 7 days as one treating course. Results： The total effective rate of the observation group was 97.2% while that of the control group was 82,8%, so there is a significant difference between them. On the first and third days the clinical manifestations were more satisfactorily improved in the treatment group than in the control group （P 〈 0.01） and on the fifth and seventh days, the comparison showed no significant difference （P〉0.05）. Conclusion： The treatment of acute bronchitis by means of the integrated traditional Chinese and western medicine primarily with pricking-cupping method on Back-shu points is of marked therapeutic effect, simple manipulation, and little untoward effects, thus claiming the unique advantage.

STANDARDIZATION OF ACU-MOXIBUSTION IS VERY IMPORTANT FOR THE INTERNATIONALIZATION AND MODERNIZATION OF ACUPUNCTUROLOGY

In the present paper, the authors present their viewpoints about the standardization of acu-moxibustion from ① orderliness of pattern-identification and acupoint prescription, ② unification of acupoint location, ③ systematization of acupoint needling, and ④ standardization of needling manipulations. The standardization is very important for the development of acupuncturology and for its popularization in the world.

The 20^(th) Anniversary of World Federation of Acupuncture-Moxibustion Societies (WFAS) and International Acupuncture Congress (First Announcement)Beijing,China

With the advent of another fruitful and golden autumn, we joyfully greet the 20^th Anniversary of World Federation of Acupuncture-Moxibustion （WFAS）. It has been growing day by day and been regarded as an important academic power in traditional medicine arena around the globe over the past twenty years under the care, cherish and support of acupuncture organizations and colleagues all over the world.

INFLUENCE OF DIFFERENT INTERVALS OF ACUPUNCTURE TREATMENT ON BONE INTENSITY IN PRIMARY OSTEOPOROSIS PATIENTS

Objective: To observe the influence of different intervals of acupuncture treatment on bone intensity in primary osteoporosis subjects. Methods: A total of 45 cases of primary osteoporosis female patients were randomly divided into 1 treatment/week (1-T/W), 2 treatments/week (2-T/W) and 3 treatments/week (3-T/W) groups, with 15 cases being in each group. Guanyuan (CV 4), Zusanli (ST 36), Sanyinjiao (SP 6), Shenshu (BL 23), Taixi (KI 3) and Pishu (BL 20) were used and stimulated with reinforcing needling manipulation. For patients with deficiency syndrome of kidney-yang, thermal acupuncture was applied to Shenshu (BL 23), and for patients with spleen-deficiency syndrome, thermal acupuncture applied to Zusanli (ST 36). Results: After 6 months’ treatment, in 1-T/W group, no apparent change of bone intensity was observed, while in 2-T/W and 3-T/W groups, the bone intensity was increased significantly (P<0.05, 0.01). Conclusion: Acupuncture plus moxibustion treatment twice or 3 times every week can obviously improve the bone intensity.

EFFECT OF ACUPUNCTURE PLUS MOXIBUSTION OF YONGQUAN POINT ON DYSCINESIA AND BLOOD RHEOLOGY IN STROKE PATIENTS

Objective: To observe the therapeutic effect of acupuncture plus moxibustion of Yongquan (KI 1) in improving stroke patients’ dyskinesia and blood rheology. Methods: 78 cases of stroke patients were treated by acupuncture and moxibustion of Yongquan (KI 1), once daily, with 20 days being a therapeutic course. Indexes of whole blood viscosity, plasma viscosity, platelet aggregation rate, packed cell volume and fibrinogen were detected before and after treatment. Changes of the functional activity were assessed using Barthel marking method. Results: After 2 courses of acupuncture treatment, all the above mentioned indexes and functional activity were improved significantly (P<0.05～0.01). Conclusion: Acupuncture and moxibustion of Yongquan (KI 1) has an active effect in improving stroke patients’ dyskinesia and blood rheology.

Fifty-Six Cases of Acne Treated by Auricular Needle-Embedding

Acne is an inflammatory disease often affecting the boys and girls at the pubescent age, with a tendency of affecting the females aged 30-45 years. We have treated 56 cases of acne by auricular needle-embedding with satisfactory therapeutic results as reported in the following.……

CLINICAL OBSERVATION ON STAGE-AIDED TREATMENT OF 62 CASES OF PERIPHERAL FACIAL PARALYSIS WITH ACUPUNCTURE,MOXIBUSTION AND CUPPING

Objective: To probe the best therapy for peripheral facial paralysis. Methods: A total of 122 cases of facial paralysis patients were randomized into treatment group (n=62, treating the disease by stages) and control group (n=60). For patients at the acute stage in treatment group, main point Yifeng (TE 17) was pricked first, followed by performing cupping and moxibustion, for patients at the resting stage, main point Hegu (LI 4) was punctured with reducing needling method, combined with other acupoints in the light of the concrete situations. For patients at the restoration stage, main point Zusanli (ST 36) was punctured with reinforcing method in combination with seven-star-needle tapping at the local affected region. Patients of control group were treated with routine method by puncturing Fengchi (GB 20), Yifeng (TE 17), Jiache (ST 6), Hegu (LI 4), Taichong (LR 3), etc.. The treatment was conducted once daily, with 10 days being a therapeutic course. Results: Following 3 courses of treatment, of the 62 cases in treatment group, 44 (70.9%) were cured, 12 (19.4%) had remarkable improvement in their symptoms and signs, 6 (9.7%) had amelioration, with the cure plus markedly effective rate being 90.3%; of the 60 cases in control group, 30 (50.0%) were cured, 12 (20.0%) had apparent improvement, 16 (26.7%) had amelioration, and the rest 2 (3.3%) failed in the treatment, with the cure plus markedly effective rate being 70.0%. Ridit analysis showed that the cure rate and cure plus markedly effective rate of treatment group were significantly higher than those of control group (P<0.05). Conclusion: Stage-aided acupuncture treatment is superior to routine treatment for facial palsy.

INFLUENCE OF ELECTROACUPUNCTURE ON ARTIFICIAL ABORTION- INDUCED SIDE EFFECTS

In the present study, the effect of electroacupuncture (EA) of acupoints of Ren, Spleen and Stomach Meridians on artificial abortion induced side effects was observed in 100 artificial abortion women. In comparison with 45 artificial abortion women in the control group (who had not accepted EA treatment), EA possessed significant effects in relieving abdominal pain, reducing vaginal bleeding duration, lowering infection rate and infertility rate after artificial abortion operation.

EFFECTS OF ELECTRO ACUPUNCTURE AND TWIRLING REINFORCING-REDUCING MANIPULATIONS ON VOLUME OF MICROCIRCULATORY BLOOD FLOW IN CEREBRAL PIA MATER

Effects of electroacupuncture (EA) and routine acupuncture with twirling reinforcing and reducing manipulation of the needle (RA) both at Zusanli point (St 36) on volume of microcirculatory blood flow in the cerebral pia mater were observed by fenestration of the cranial bone and laser Doppler microcirculatory blood flow analyser. Results showed that both RA and EA could increase the volume of microcirculatory blood flow in the cerebral pia mater; and that the increase in the EA group was superior to that in RA group. This suggests that a moderate and effective stimulation is a key to the production of a regulative effect on the organism.