“治痿独取阳明”指导下康复锻炼配合针刺、电针改善脑卒中患者足下垂的临床研究

目的:探究“治痿独取阳明”原则指导下应用针刺、电针合康复锻炼改善脑卒中患者足下垂的疗效。方法:选取2016年10月~2020年11月在我科诊断为脑卒中后遗症期并足下垂的382例患者,并随机分为对照组和观察组各191例。对照组采用康复锻炼治疗,2次/天;观察组加用针刺阳明经相应穴位,同时电针刺激足三里和阳陵泉,1次/天,5~6次/周。治疗8周后比较两组下肢神经肌电活动差异,应用Fugl-Meyer运动量表(下肢部分)及Brunnstrom分期比较两组疗效差异。结果:与对照组比较,观察组胫神经、腓总神经运动神经末端潜伏期(DML)、感觉神经传导速度(SCV)和电位(SNAP)均显著改善(P<0.01),运动神经传导速度(MCV)改善(P<0.05)。相比对照组,观察组下肢Fugl-Meyer运动量表评分及Brunnstrom分期均较高(P<0.05)。结论:“治痿独取阳明”理论指导下的综合治疗可以有效治疗脑卒中后遗症期的足下垂。

Enhanced expression of epidermal growth factor receptor gene in gastric mucosal cells by the serum derived from rats treated with electroacupuncture at stomach meridian acupoints

AIM: To investigate the effect of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expression of epidermal growth factor receptor (EGFR) gene in gastric mucosal cells. METHODS: The stress-induced gastric mucosal injury in rat model was established by water-immersion and restrained stress methods. 52 rats were randomly divided into: normal group (n = 8), model group (n = 8), model serum group (n = 12), stomach serum group (n = 12), and gallbladder serum group (n = 12). The gastric mucosal cells were separated by pronase-EDTA digestion method and incubated with serum. The EGFR gene expression in gastric mucosal cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: Compared with normal group (0.6860 ± 0.0594), the serum derived from rats of the stomach group (1.2272 ± 0.0813, P = 0.00 < 0.01) and gallbladder group (0.9640 ± 0.0387, P = 0.00 < 0.01) had a tendency to enhance the EGFR gene expression in gastric mucosal cells. Such tendency existed in the model group (0.7104 ± 0.0457) but with no signifi cant difference (P = 0.495 > 0.05) and in model serum group (0.8516 ± 0.0409) with an extremely obvious difference (P = 0.001 < 0.01). Furthermore, the EGFR gene expression in stomach serum group was significantly higher than that in gallbladder serum group (P = 0.00 < 0.01). CONCLUSION: The present study shows that serumderived from rats treated with electroacupuncture at stomach meridian acupoints can distinctly increase the EGFR gene expression of gastric mucosal cells. Therefore, there is certain meridian specificity in the serum, which could provide a proof for the TCM theory “particular relation between meridian and internal organ”.

STUDY ON SCALP ELECTROACUPUNCTURE TREATMENT OF APOPLEXY AND ITS INFLUENCE ON HEMORHEOLOGY IN ISCHEMIC APOPLEXY PATIENTS

Objectives: To compare the therapeutic effect of scalp electroacupuncture with that of conventional body acupuncture for apoplexy (wind stroke) and to observe the influence of both acupuncture therapies on hemorheology of ischemic apoplexy.Methods: 183 patients, whose diseases are attributed to apoplexy according to the diagnostic criteria, are randomly divided into treatment group (93 cases) and control group (90 cases) and treated respectively with scalp electroacupuncture and conventional body acupuncture. 11 items of hemorheology of patients’ blood samples are tested with NXE-1 Viscometer which are taken in the early morning before and after treatment under fasting condition. Results: Both scalp electroacupuncture and conventional body acupuncture exert therapeutic effect for apoplexy, but the effect of scalp electroacupuncture is obviously better than that of conventional acupuncture (P< 0.05 ); both treatment group and control group are statistically significant in reducing plasma viscosity, high shear reduced viscosity, low shear reduced viscosity, erythrocyte sedimentation rate, hematocrit and fibrinogen, (P<0.01 and 0.05); while the treatment group is statistically significant in reducing whole blood viscosity, ratio of whole blood viscosity, erythrocyte index of rigidity and electrophoresis time of erythrocyte (P<0.01～ 0.05), but the control group is not significant statistically in influencing these indexes (P>0.05).

OBSERVATION ON THERAPEUTIC EFFECT OF ELECTROACUPUNCTURE HEAD PONINT-THROUGH-POINT BY PENETRATING TWO POINT ON ISCHEIC STROKE

Objective To probe methods and therapeutic effects of Head Acupuncture on ischemic stroke. Methods One hundred and twelve cases were randomly divided into observation group (head acupuncture group) and control group (body acupuncture group). After treatment of 28 days, changes of linguistic function and paralysis level of limbs before and after treatment were investigated. Results The total effective rate was 96.6% in the observation group and 90.7%, in the control group, with a significant difference between the two groups (P<0.05). Conclusion The electroacupuncture head point-through-point by penetrating two point therapy is one of effective methods for ischemic stroke.

Electro-acupuncture Treatment for the Upper Segment Ureterolithiasis Under B-Ultrasonography

In order to explore the effects of the local strong stimulation generated by electro-acupuncture for treatment of the upper segment ureterolithiasis,a controlled study was carded out among the treatment group (electro-acupuncture with strong stimulation),the control group Ⅰ(medication) and the control group Ⅱ (conventional acupuncture).The results showed that the differences in the cure rate and the total effective rate between the treatment group and the two control groups were significant in statistical analysis (P<0.05 and P<0.01 respectively).This indicates that better therapeutic effects can be obtained by the local strong stimulation generated by electro-acupuncture for treatment of the upper segment ureterolithiasis.

A SURVEY ON ACUPUNCTURE TREATMENT OF PERIPHERAL NERVE INJURY

By peripheral nerve injury, we mean theloss of neurosensory and neuromotor functionsinduced by various causative factors,manifesting paralysis of the limbs andmuscular atrophy. It falls into the category ofinjury of the muscle and tendon, and flacciditysyndrome in TCM. The following is asummary of documents in the recent 20

ELECTROACUPUNCTURE TREATMENT OF 176 CASES OF LUMBAR INTERVERTEBRAL DISC PROTRUSION

In the present paper, 176 cases of lumbar intervertebral disc protrusion are treated with electroacupuncture (EA) and topical heat irradiation. Local tender-point is used as the main acupoint, combined with Tunzhong, Tiaoyue, Weizhong (BL 40) and Yanglingquan (GB 34). The treatment is conducted once daily, with 15 sessions being a therapeutic course. Following treatment, of 176 cases, 46 (26. 1%) are cured, 90 (51. 1%) have prominent improvement, 35 (19.8%) have amelioration and 5 (2.8%) have no apparent changes, with a total effective rate of 97.0%.

EFFECT OF ELECTROACUPUNCTURE OF DUMAI-ACUPOINTS ON CEREBRAL NO AND BLOOD ENDOTHELIN CONTENTS IN RATS WITH ACUTE CEREBRAL ISCHEMIA

Thirty Wistar rats were randomly and evenly divided into control group, cerebral ischemia group and ischemia + electroacupuncture (EA) group. The bilateral common carotid arteries were occluded to induce acute cerebral ischemia. Nitric oxide (NO) and endothelin (ET)contents in the cerebral tissues and blood were measured under normal condition, immediately after ischemia and following EA. Results showed that after acute cerebral ischemia NO and ET contents in the cerebral tissues increased significantly (P<0.01) while serum ET increased and serum NO lowered obviously (P<0.05). Following EA of Baihui (GV 20) and Dazhui (GV 14), both NO and ET in cerebral tissues and serum turned to normal basically. It showed that EA could protect the cerebral tissues from injury induced by ischemia, NO and ET might participate in the modulation process of EA.

TREATMENT OF 100 CASES OF ACUTE URTICARIA WITH ELECTROACUPUNCTURE

Objective: To observe the therapeutic effect of clinical treatment of acute urticaria chiefly by electroacupuncture (EA). Methods: A total of 180 outpatients with acute urticaria were randomized into treatment group and control group. 100 cases in the treatment group were treated with EA at Quchi (曲池 LI 11) and Xuehai (血海 SP 10) as the main points which were supplemented with Dazhui (大椎 GV 14), Fengchi (风池 GB 20), Geshu (膈俞 BL 17), Pishu (脾俞 BL 20), etc. 80 cases in the control group were managed by chlorpheniramine maleate and Vitamin C. Results: After 3 days’ treatment, of the 100 and 80 cases in treatment and control groups, 79 and 53 were cured, 10 and 6 markedly effective, 5 and 8 effective, and 6 and 13 failed, with the effective rates being 94.00% and 83.75% respectively. The therapeutic effect of electroacupunture was significantly superior to that of medication(P<0.05). Conclusion: The method of electroacupuncture at Quchi (曲池 LI 11), Xuehai (血海 SP 10),etc. was a more effective therapy for acute urticaria.

EFFECT OF ELECTROACUPUNCTURE ON RED BLOOD CELL IMMUNE AND T-CELL SUBGROUP IN THE RAT

In the present study, the effect of electroacupuncture (EA) on immune system was observed in the rat by using micro- whole blood direct immunofluorescence staining assay to detect changes of the peripheral blood T lymphocyte subgroup and employing red blood cell (RBC) C 3b receptor- yeast rosette test and red blood cell-IC rosette test to analyze erythrocytic immune function. Results showed that after EA of “Zusanli" (ST 36), CD+ 4, RBC-C 3bRR and RBC-ICR in the peripheral blood of the normal rats increased significantly while CD+ 8 had no any considerable changes and a positive correlation between CD+ 4 and RBC-C 3bRR was found. In immunosuppression model rats, the values of CD+ 4 and RBC-C 3bRR were obviously lower than those of the normal control group while CD+ 8 had no any striking changes; but after EA treatment, there were no evident differences between EA group and normal control group in the above-mentioned indexes. There were also no any significant differences between non-acupoint group and normal control group in those indexes. Results suggest that EA of “Zusanli" (ST 36) can raise T cell immune function and RBC adhesion function in both normal rats and immunosuppression model rats, both of which present a positive correlation.

INFLUENCE OF ELECTROACUPUNCTURE ON ARTIFICIAL ABORTION- INDUCED SIDE EFFECTS

In the present study, the effect of electroacupuncture (EA) of acupoints of Ren, Spleen and Stomach Meridians on artificial abortion induced side effects was observed in 100 artificial abortion women. In comparison with 45 artificial abortion women in the control group (who had not accepted EA treatment), EA possessed significant effects in relieving abdominal pain, reducing vaginal bleeding duration, lowering infection rate and infertility rate after artificial abortion operation.

EXPERIENCE IN THE POINT-SELECTION FOR ELECTRO-ACUPUNCTURE

Point selection along the course of meridians is the basic principle for acupuncture prescription and selection of points. In application, there are three categories of point selection: selection of local and adjacent points; selection of distant points, and selection of symptomatic points. Based on the

Acupuncture treatment for improving anxiety status in patients with primary insomnia:a systematic review and meta-analysis

Objective:To evaluate the clinical efficacy of acupuncture in the treatment of anxiety state in patients with primary insomnia(PI).Methods:Randomized controlled trials of acupuncture treatment for PI patients with an anxiety state in Web of Science,Cochrane Library,PubMed,Excerpta Medica Database(EMBASE),China National Knowledge Infrastructure(CNKI),Wanfang Data Knowledge Service Platform(Wanfang),and Chongqing VIP Database(VIP)were retrieved by computer.The retrieval time was from each database’s inception to December 30,2022.Data extraction and evaluation were performed for the included studies.The Cochrane risk of bias assessment tool was used to assess the risk of bias in each article.Meta-analysis of valid data was performed using the RevMan 5.4 software.If the outcome indicator was a categorical variable,relative risk(RR)was used as the effect size.If it was a continuous variable,mean difference(MD)was used to calculate the effect size.Each effect size was expressed as a 95%confidence interval(CI).P<0.05 was considered to indicate a statistically significant difference.Results:A total of 18 studies were included,comprising a total of 1198 patients.The findings of the meta-analysis showed that electroacupuncture had a significant advantage in improving the Hamilton anxiety scale(HAMA)score than benzodiazepines[MD=-1.61,95%CI(-2.17,-1.06),P<0.001].Acupuncture was superior to sham acupuncture[MD=-14.90,95%CI(-20.39,-9.41),P<0.001]and benzodiazepines[MD=-3.39,95%CI(-4.67,-2.12),P<0.001]in reducing the self-rating anxiety scale(SAS)score.Acupuncture was superior to sham acupuncture in reducing the insomnia severity index(ISI)score[MD=-5.61,95%CI(-6.63,-4.89),P<0.001].Acupuncture was superior to benzodiazepines[MD=0.84,95%CI(-1.42,-0.25),P=0.005]and sham acupuncture[MD=-8.39,95%CI(-8.39,-7.86),P<0.001]in improving the Pittsburgh sleep quality index(PSQI)score.Acupuncture had a better effective rate than benzodiazepines[RR=1.16,95%CI(1.08,1.25),P<0.001]and sham acupuncture[RR=8.94,95%CI(4.63,17.25),P<0.001]in treating PI.Conclusion:Acupuncture or electroacupuncture has certain therapeutic advantages over benzodiazepines and sham acupuncture in the treatment of anxiety in PI patients.However,more high-quality randomized controlled trials are needed for further verification.

Effect of electroacupuncture on expression of TNF-α and IL-1β in substantia nigra in rats with Parkinson's disease

Objective To observe mechanisms of electroacupuncture （EA） on treating Parkinson＇ s disease （PD）. Methods Fifty SD rats were randomly divided into 4 groups： a normal control group （Norm）, a sham operation group （Sham）, a model group （Mod） and an electroacupuncture （EA） group. EA at ＂Fengfu＂ （风府 GV16） and ＂Taichong＂ （太冲 LR 3） was applied once daily for 2 weeks in Group EA, and no treatment was given to the animals in the other three groups. PD model was established by using 6-hydroxydopamine. Numbers of tyrosine hydroxylase （TH）, tumor necrosis factor-alpha （TNF-α）and interleukin-1 beta （IL-1β） positive reactive cells in substantia nigra （SN） were counted by using immunohistochemical methods. Results The total TH positive neurons in Group Mod were obviously less than those in Group Norm and Group Sham （P〈0.01）, and the numbers of TNF-α and IL-1β positive cells in Group Mod increased more significantly than those in Group Norm, and Group Sham （P〈0.01）. The total TH positive neurons in Group EA were obviously more than those in Group Mod （P〈0.01）, and the numbers of TNF-α and IL-1β positive cells in Group EA were obviously less than those in Group Mod （P〈0.01）, indicating that EA could markedly lower the contents of such pre-inflammatory factors like TNF-α and IL-1β in SN of experimental rats with PD. Conclusion EA may provide its protective functions on dopaminergic neurons by way of alleviating inflammatory reactions in SN of rats with PD.

Electroacupuncture combined with auricular acupressure in the treatment of nervous tinnitus

Objective To observe the clinical efficacy of electroacupuncture combined with auricular acupressure in treatment of nervous tinnitus. Methods One hundred and four patients with nervous tinnitus were randomly divided into a group A and a group B according to the method of random number table, with 52 patients in each group. In the group A, acupuncture at Yìfēng（翳风 TE 17）, Tīnggōng（听宫 SI 19）, Tīnghuì（听会 GB 2）, ěrmén（耳门 TE 21） and Fēngchí（风池 GB 20） combined with auricular acupressure, such as Shènshàngxiàn（肾上腺 TG 2p）, Jiāog？n（交感 AH6a）, Nèifēnmì（内分泌 CO18）, Nèi？r（内耳 LO 6）, Wài？r（外耳 TG1u）, Shèn（肾 CO10） and Gān（肝 CO12） were applied; in the group B, intravenous injection with compound Dānshēn（丹参 Radix et Rhizoma Salviae Miltiorrhizae） dissolved in 250 m L of 0.9% Na Cl injection were applied, combined with oral administration of 10 mg of Vitamin B1 and 0.5 mg of mecobalamin tablets. After treatment for three courses in the two groups, efficacy was evaluated based on Reference Standards of Severity, Assessment and Efficacy Evaluation of Tinnitus. Results In the group A（75 ears with nervous tinnitus）, 30 ears were cured, markedly effective was seen in 21 ears, and effective in 22, and the total effective rate was 97.4%; in the group B（76 ears with nervous tinnitus）, 17 ears were cured, markedly effective was seen in 20 ears, and effective in 23, and the total effective rate was 79.0%. The total effective rate in the group A was obviously superior to that in the group B, and the difference between the two groups was statistically significant（P〈0.05）. Compared before the treatment, the score of tinnitus severity both reduced in two groups（both P〈0.05）, and the reducing amplitude of score in the group A was greater than that in the group B（P〈0.05）. Conclusion Electroacupuncture combined with auricular acupressure in treatment of nervous tinnitus is worthy of clinical promotion and application since it has significant efficacy, which is obviously superior to drug therapy, and without adverse effect.

Controlled observation on the efficacy of acupuncture and western medicine on diarrhea-type irritable bowel syndrome

Objective To compare the difference in the therapeutic effects on diarrhea- type irritable bowel syndrome （D-IBS） between acupuncture based on soothing the liver and strengthening the spleen and western medicine. Methods Seventy cases were randomized into an acupuncture group and a western medicine group, 35 cases in each one. In the acupuncture group, the conventional acupuncture was applied at Tianshu {天枢 ST 25）, Zusanli （足三里 ST 36）, Shangjuxu {上巨虚 ST 37）, Sanyinjiao （三阴交 SP 6）, Taichong （太冲 LR 3） and so on. Electric stimulation was supplemented at bilateral ST 25. The treatment was given once a day, 3-4 treatments in a week. In the western medicine group, pinaverium bromide （dicetel） was prescribed for oral administration, 50 mg each time, three times a day. Four weeks made one session in the two groups. Separately, before treatment and after 1 session treatment, the integral of clinical symptom and the score in the scale of the quality of life measure for irritable bowel syndrome （IBS-Q OL） were observed for the patients in the two groups. The efficacy and recurrence rate were assessed in the two groups. Results The symptom integral and IBS-QOL score in the two groups were improved significantly after treatment Call P〈0.01）, and the improvements in the acupuncture group were superior to that in the western medicine group （P〈0.01）. The effective rate was 94.3% {33/35） in the acupuncture group, which was superior to 77.1% （27/35） in the western medicine group （P〈0.01）. In 3 months, the recurrence rate was 36.4% （12/33） in the acupuncture group, which was lower apparently than 72.0% （18/25） in the western medicine group （P〈O.01）. Conclusion Acupuncture therapy based on soothing the liver and strengthening the spleen achieves the superior efficacy on D-IBS as compared with western medication, pinaverium bromide. This therapy improves apparently the quality of life of the patients and is lower in recurrence rate.

Observation on the therapeutic effect of electroacupuncture on simple obesity of gastrointestinal heat pattern/syndrome

Objective To compare the therapeutic effect on simple obesity of gastrointestinal heat type between electroacupuncture （EA） and conventional acupuncture （CA）. Methods Ninety cases of simple obesity of gastrointestinal heat type were randomized into an EA group and a CA group, 45 cases in each one. In the EA group, CA was applied at Neiting （内庭 ST 44）, Shangjuxu （上巨虚 ST 37）, Xiajuxu （下巨虚 ST 39）, Fenglong （丰隆 ST 40）, Tianshu （天枢 ST 25）, Zusanli （足三里 ST 36）, YTnlingquan （阴陵泉 SP 9） and Ouchi （曲池 LI 12）. The electric stimulation was attached at ST 37, ST 44, ST 25 and ST 40. In CA group, the simple acupuncture was applied at the same points as the EA group. The treatment was given once every two days, 22 treatments made one session and 3 sessions were required totally in the two groups. The changes in body mass, obesity degree, body mass index （BMI） and body fat percentage （F%） were observed in the patients before and after treatment. The therapeutic effects on weight loss and fat loss were compared between the two groups. Results After treatment, the weight, obesity degree, BMI and F% were all reduced apparently in the patients of the two groups （all P〈0.01）. After the 2st session of treatment, concerning to the effect on weight loss, the total effective rate in the EA group was superior to that in the CA group [95.6% （43/45） vs 77.8% （35/45）, P〈0.05]. The effects were not different apparently between the two groups in the later 2 sessions of treatment （all P〉0.05）. The effect on fat loss was not different significantly in statistics between EA and CA （all P〉0.05）. Conclusion Either EA or CA achieves the significant effect on simple obesity. The therapeutic effect of EA on weight loss in the early stage is obviously superior to that of CA.

Protecting action of acupuncture-drug compound anesthesia with different frequency electroacupuncture on stress reaction in patients of pneumonectomy

Objective To observe the protective effect of acupuncture-drug compound anesthesia with different frequency electroacupuncture（EA） on stress reaction in patients of pneumonectomy and to explore potential mechanisms.Methods Eighty patients scheduled for pneumonectomy were randomly divided into four groups,group A,B,C and D,20 cases in each group.General anesthesia and single lung protective mechanical ventilation were carried out in all the groups.Acupuncture was given at Hòuxī（后溪 SI 3）,Zhīgōu（支沟TE 6）,Nèiguān（内关 PC 6）,and Hégǔ（合谷 LI 4） 30 min before general anesthesia,and the acupuncture needles were connected with Han＇s acupoint nerve stimulator（HANS-200） in all the groups,but the acupuncture needles without needle bodies were pasted on the acupoints and EA was not given in the group A.2 Hz continuous wave,100 Hz continuous wave and 2 Hz/100 Hz EA were given in the group B,C and D respectively.The supplementary amount of anesthesia medicine,heart rate（HR） and mean arterial pressure（MAP） during pneumonectomy,and CD4^＋/CD8^＋ in venous blood before and one day after the surgery,and the contents of epinephrine（E） and cortisol（Cor） in plasma after entering the operating room and before turning out the operating room were detected.Results ① During the operation,supplementary amounts of Fentanyl in the group B and C were lower than those in the group A and D（P〈0.05,P〈0.01）.② The MAP in the four groups at tracheal intubation（T1） all were higher than those before anesthesia（T0）（all P〈0.01）,and the ascending extents in the group B,C and D were lower than that in the group A（all P〈0.01）;HR at T1 in the group A was higher than that at T0（P〈0.05）,while no significant change in the other groups（all P〈0.05）,and the ascending extents in the group B and D were lower than that in the group A（both P〈0.05）;MAP and HR at the other moments in all the groups were stable.③ CD4^＋/CD8^＋ in the group A after pneumonectomy was lower than that before the surgery（P〈0.05）,while no significant change in the other groups（all P〈0.05）.④ Contents of E and Cor after the operation were significantly increased in all the groups（all P〈0.01）,and the ascending extent of E content in the group D was lower than that in the group A（P〈0.05）;the ascending level of Cor in the group B and D was lower than that in the group A（P〈0.01）,and in the group B was lower that in the group C and D.Conclusion Acupuncture-drug compound anesthesia can attenuate the stress reaction so as to protect organs under the condition of less or same narcotic amount and can alleviate the fluctuation of MAP and HR at tracheal intubation and stabilize CD4^＋/CD8^＋ after pneumonectomy.Among them,2 Hz and 2 Hz/100 Hz EA have better effects.

A case of amyotrophic cervical spondylosis

A case of amyotrophic cervical spondylosis was healed by using acupuncture in combination with electroacupuncture, needling was carried out on fengchi（风池 GB 20）, Tianzhu （天柱 BE 10）, Dazhui （大椎 GV 14）, Jianjing （肩井GB 21）, Zhiyang （至阳 GV 9）, Jianzhen （肩贞SI 9）, Jianyu （肩髃 El 15）, Shousanli （手三里LI 10）, Houxi （后溪SI 3）, Kunlun （昆仑 BE 60） and Shugu （束骨 BL 65）. BL 10 on the left side and LI 10 on the right side, GB 20 on the right side and GV 9 were connected for electroacupuncture, once a day, 12 treatments as one treatment course, a interval of 5 days was set between two treatment courses, and totally 6 treatment courses were carried out. The patient was clinically healed after the treatment and the disease did not recur during the follow-up for six months.

Optimization of acupuncture treatment programs for facial paralysis

Objective To optimize acupuncture treatment programs for facial paralysis.Methods Sixty-three cases of facial paralysis were randomly grouped according to orthogonal design L9（3）4 table,and treated by different combined programs of 4 factors,A（acupuncture opportunity）,B（combination of points）,C（stimulating quantity）,D（electroacupuncture time） and 3 levels,and changes of the functional score of the facial nerve were observed,and the different combined programs of the 4 factors,A（acupuncture opportunity）,B（combination of point）,C（stimulating quantity）,D（electroacupuncture time） and 3 levels influencing acupuncture therapeutic effect on facial paralysis were optimized.Results B（combination of points） and D（electroacupuncture time） were obvious factors（P0.05）,among them,B（combination of points） was the most main influencing factor;among the 3 levels of B（combination of points） and D（electroacupuncture time）,B3 （alternately needling two groups of acupoints） and D3 （electroacupuncture treatment at sparse-dense wave for 30 min） were the best.Conclusion Electroacupuncture at sparse-dense wave for 30 min,alternately needling two groups of acupoints is the best program for treatment of facial paralysis.