AIM: To determine the role of acupuncture therapy in treating experimental gastric ulcer in rats.METHODS: Twenty-eight adult male Sprague-Dawley rats were randomly divided into four groups (pre-acupuncture group; acup...AIM: To determine the role of acupuncture therapy in treating experimental gastric ulcer in rats.METHODS: Twenty-eight adult male Sprague-Dawley rats were randomly divided into four groups (pre-acupuncture group; acupuncture group; paradistance-acupuncture group;and control group), and pre-acupuncture, paradistanceacupuncture, and control groups received 5 μL acetic acid (200 mL/L HAc) injection after a same course of electroacupuncture (EA) treatment (4 Hz, 0.6 mA, 0.45 ms,45 min for 4 d). The rats in these three groups recovered within 4 d. The acupuncture group received EA therapy for 4 d, after HAc injection. The stomach was dissected to compare the pathological structures of ulcer. Also c-Fos activation in the nuclei of solitary tract (NTS) was observed under microscope after regular immunohistochemistry staining of brain stem sections.RESULTS: The number of ulcers was different among the four groups, especially between control group and paradistance-acupuncture group or pre-acupuncture group. In the latter group, the number of ulcers was much less. The gastric ulcer area was consistent with the histopathological results, indicating that pre-acupuncture had an obvious therapeutic effect on gastric ulcers.Acupuncture had a very modest effect and paradistanceacupuncture had no effect on gastric ulcers. No therapeutic effect was found in the control group. Fos-Li neurons in NTS induced by noxious gastric ulcer showed a significant difference between pre-acupuncture and control groups.CONCLUSION: Acupuncture before ulceration can obviously alleviate ulcer. The production of c-Fos proves that the vagus nerve mediates the induction of c-Fos in nuclei of solitary tract following experimental ulceration,suggesting that parasympathetic afferents promote the process of noxious visceral stimulation.展开更多
Objective: To explore the possible mechanisms of "JIN San Zhen" (JIN’s 3 acupoints groups) in treatment of mental retardation. Methods: A total of 38 female mice were randomly divided into normal control (n...Objective: To explore the possible mechanisms of "JIN San Zhen" (JIN’s 3 acupoints groups) in treatment of mental retardation. Methods: A total of 38 female mice were randomly divided into normal control (n=10), sham operation (n=10), model (n=8) and acupuncture (n=10) groups. "Si Shen Zhen" [4 points around "Baihui" (GV 20): 0.3 cm anterior, posterior, left and right to GV 20 respectively], and "Zhi San Zhen" ["Shenting" (GV 24) and bilateral "Benshen" (GB 13)] of "JIN San Zhen" series are used. The memory ability, AChE positive fiber density and mono amines transmitters of the brain are used as the indexes. Results: After 8 days’ treatment, cerebral ischemia induced mental retardation (decline of learning memory ability) was improved significantly shown by Y maze test (P<0.05-0.01), simultaneously, AChE positive fiber density of the frontal cerebral cortex and hippocampus, serotonie (5 HT), noradrenaline (NA) and dopamine (DA) contents of the brain increased significantly (P<0.05). Conclusion: Acupuncture induced increase of AChE, 5 HT, NA and DA in the brain may contribute to the effect of acupuncture in improving the memory capability of the RD mice.展开更多
Objective.. To compare the mechanisms of analgesia induced by four kinds of acupunture therapies at the trypothalamic level in adjuvant arthritic rats. Methods： Forty-eight SD rats were randomized into normal, model,...Objective.. To compare the mechanisms of analgesia induced by four kinds of acupunture therapies at the trypothalamic level in adjuvant arthritic rats. Methods： Forty-eight SD rats were randomized into normal, model, electroacupuncture （EA）, filiform needle （FN）, pricking blood-letting （BL） and point injection （PI） groups, with 8 cases in each. EA （20-100 Hz, 2-4 V and duration of 20 min）, FN, BL PI were respectively applied to “Kunlun” （昆仑 BL 60）. Arthritis model was established by injecting complete Freund＇s adjuvant （0.1 mL） into the rat＇s right foot pad. Behavioral reactions, pain threshold （latancy of tail flick to heat stimulation） and local swelling severity （foot volurne） were detected; the cordents of β-endorphin （β-EP） and α- drenocortiootropin （ACTH） were assayed with radioimmunoassay; and the expression of pro-opi-omelanocortin （POMC） mRNA in hypot were determined with hybridization method. Results： The pain threshold was significantly enhanced by all the four kinds of acupuncture therapies, and the effects of EA and PI were more obvious （P〈0.05, P〈0.01）. The severity of local swelling was markedly alleviated by all the four kinds of acupuncture therapies （ P〈 0.01 ） without significant differences among them （P 〉 0.05）. The content of β-EP in the hypothalamus was obviously elevated by EA and FN （P〈 0.05, P 〈 0.01 ）, and the effect of EA was more obvious, but no marked effect of BL or PI on β-EP was found （ P 〉0.05）. The content of ACTH in hypothalamus was considerably elevated by PI （P〈0.05）, but not by the other three therapies. The expression of PCMCmRNA in hypothalamus was significantly strengthened by EA and FN （P〈0. 01）, but not by the other two therapies. Conclusion：EA, flliform needle, blood-letting and point-injection all can produce analgesic effect in adjuvant arthritis rats, the effect of EA and filiform needie may be related to their resultant increase of hypothalamic β-EP, and that of point-injectiON related to the increase of hypothalamic ACTH level.展开更多
AIM: To study the nervous-pathways of Fengch'ih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP). METHODS: Fifty Wistar rats were randomly divided into 1, 2,3,4, an...AIM: To study the nervous-pathways of Fengch'ih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP). METHODS: Fifty Wistar rats were randomly divided into 1, 2,3,4, and 5 d groups, and every group had 10 animals. HRP (30% aqueous solution) was injected into a Fengch'ih. Serial, transverse or capital, 40 urn sections of the cervical spinal ganglia, cervical and thoracic spinal cord segment and brain were cut on a cryotome. Sections were incubated for HRP histochemistry according to the tetramethylbenzidine (TMB). Part of the sections were counterstained with neutral red. RESULTS: After 1 d of survival times, many labeled cell bodies were found in 1-4 cervical spinal ganglia, anterior horn of 1-4 cervical spinal cord, ventromedial division of facial nucleus, accessory facial nucleus ipsilaterally. With increasing survival times, the intensity of labeled cells were slightly decreased. CONCLUSION: Fengch'ih may bring into full play its effect by correlation of posterior ear branch of facial nerve and anterior branch of 2-3 cervical nerve with 1-4 cervical the anterior horn of the spinal cord, ventromedial division of facial nucleus, accessory facial nucleus.展开更多
PURPOSE: To observe the effects of acupuncture on the pituitary-thyroid axis in rabbits with fracture. METHOD: 36 male rabbits with a 3-mm width experimental defect in the middle segment of the left radius were random...PURPOSE: To observe the effects of acupuncture on the pituitary-thyroid axis in rabbits with fracture. METHOD: 36 male rabbits with a 3-mm width experimental defect in the middle segment of the left radius were randomly divided into the treatment group and control group, and the changes in serum T3, T4 and TSH contents in different weeks were investigated. RESULTS: Serum T3, T4 and TSH contents in the treatment group all were higher than those in the control group, with statistically significant differences in the contents of T4 and TSH in the second week and T3 content in the fourth week (P展开更多
Objective： To verify two hypotheses： 1 ） Acupuncture can increase the re-distribution of tetramethylpyrazine （ligustrazine） hydrochloride （TMPH） to the target organs in adjuvant arthritic rats. 2） Cyclic Adeno...Objective： To verify two hypotheses： 1 ） Acupuncture can increase the re-distribution of tetramethylpyrazine （ligustrazine） hydrochloride （TMPH） to the target organs in adjuvant arthritic rats. 2） Cyclic Adenosine-3＇, 5＇-menophesphete （cAMP） is one of the guidance cues involved in this event. Methods： A total of 40 Wistar rats were randomized into blank control （BC）, model （MO）, medication （ME）, Acupuncture （Acu） and Acu＋ ME groups, with 8 cases in each group. Arthritis model was established by subcutaneous injection of complete Freund＇ s adjuvant （0.1 mL） into the rat＇ s foot pad. Electrcacupuncture （ 15 Hz, 0.2-0.3V） was applied to bilateral “Daling”（大陵 PC 7）, “Taichong” （太冲 LR 3） and “Xiguan” （膝关 LR 7） for 20 min, and the contents of TMPH in the heart, liver, kidney and the foot （affected focus） tissues and the contents of cAMP in the heart and liver tissues were determined with high performance liquid chromatography and radioimmunoassay techniques respectively. Results： Oornpared with medication group, the TMPH contents in the heart, liver, kidney and the affected foot tissues in Acu-ME group was obviously higher （ P〈0.05, P〈0.01 ）. In comparison with ME and Acu groups, the cAMP contents in the heart and liver in Acu＋ ME group was also significantly higher （P〈 0.05, P 〈 0.01 ）. No significant differences were found between ME and Acu ＋ ME groups in TMPH content of kidney and between Acu＋ ME and BC groups in cAMP contents in both liver and heart tissues. Conclusion： Acupuncture may promote the target-tropism distribution of ligustrazine, and cAMP may play an important role in it.展开更多
Objective： This study aimed to prove acupuncture therapy exerted an obvious clinical efficacy in the treatment of peripheral facial neuritis in acute phase. Methods： 60 clinically collected cases were chosen in the ...Objective： This study aimed to prove acupuncture therapy exerted an obvious clinical efficacy in the treatment of peripheral facial neuritis in acute phase. Methods： 60 clinically collected cases were chosen in the study and randomly divided into control group and treatment group. In control group, dexamethasone, vitamin B, vitamin B 12, and mecobalamine were applied in acute phase, and then acupuncture therapy was combined in remission. In treatment group, western medicine combining with acupuncture therapy was used in acute phase, and three courses of treatment were totally applied. Results： The final conditions of two groups were good, and the grouping was balanced, so the comparability between them was good （P〉0.05）; the curative effects of two groups were significantly different： the effect of treatment group was better than that of control group （P〈0.01）. Conclusion： The acute phase was necessarily grasped to apply acupuncture therapy to the treatment of peripheral facial neuritis, and the curative effect would be more significant if acupuncture therapy was more early used.展开更多
Effects of electroacupuncture (EA) and routine acupuncture with twirling reinforcing and reducing manipulation of the needle (RA) both at Zusanli point (St 36) on volume of microcirculatory blood flow in the cerebral ...Effects of electroacupuncture (EA) and routine acupuncture with twirling reinforcing and reducing manipulation of the needle (RA) both at Zusanli point (St 36) on volume of microcirculatory blood flow in the cerebral pia mater were observed by fenestration of the cranial bone and laser Doppler microcirculatory blood flow analyser. Results showed that both RA and EA could increase the volume of microcirculatory blood flow in the cerebral pia mater; and that the increase in the EA group was superior to that in RA group. This suggests that a moderate and effective stimulation is a key to the production of a regulative effect on the organism.展开更多
Objective:To probe into the most effective site,extra-ordinary point,acupoint and channel for regulating reproductive endocrine function by means of the study on acupuncture activating the gonadotropin-releasing hormo...Objective:To probe into the most effective site,extra-ordinary point,acupoint and channel for regulating reproductive endocrine function by means of the study on acupuncture activating the gonadotropin-releasing hormone neurons(GnRH) in hypothalamus.Methods:Female SD rats of reproductive age were used,and the in vivo study on GnRH neurons in hypothalamus was made with mimic sexual stimulation and feedback regulation.The neuron-activating effects of the acupoints on the channels pertaining to the zang-and fu-organs related with reproductive endocrine and the extra-ordinary points in different regions were studied using the discharge of the neuron as index,and then the neurons were labeled with horseradish peroxidase(HRP) .Results:Acupuncture was given at two acupoints each on the three yin channels of foot,the three yang channels of foot,the Conception Vessel and the Governor Vessel.The order of the mean increasing percentage in the hypothalamic GnRH neuron electric activity was:the Gallbladder Channel>the Spleen Channel>the Stomach Channel/the Bladder Channel/the Conception Vessel>the Liver Channel>the Kidney Channel>the Governor Vessel;for different acupoints,it was:Guanyuan(CV 4) >Sanyinjiao(SP 6) >Zusanli(ST 36) >Daimai(GB 26) /Yanglingquan(GB 34) >Shenshu(BL 23) >Weizhong(BL 40) >Yaoyangguan(GV 3) /Liangmen(ST 21) /Fujie(SP 14) >Qimen(LR 14) /Yingu(KI 10) >Tangzhong(CV 17) /Zhiyang(GV 9) ;for different positions,it was:the lower abdominal part/the lower limb part>the thoracodorsal part;for the extra-ordinary points,it was:Zigong(EX-CA1) >Dannang(EX-LE6) /Yaoyan(EX-B7) >Baichongwo(EX-LE3) /Qianzheng>Jingbi/Bizhong/Taiyang(EX-HN5) >Erbai(EX-UE2) /Dingchun;and for the distribution sites of the extra-ordinary points:lower abdominal region>the lower limb region>the craniofacial region>the upper limb region/the thoracodorsal region.Conclusion:For regulating the reproductive endocrine function,the acupoints located at the same neural segment with the reproductive organ should be selected as the main points,and it is necessary to combine with syndrome differentiation of the viscera and channels.展开更多
Objective： To observe the effect of electroacupuncture （EA） on tumor necrosis factor-α （TNF-α） and vascular endothelial growth factor （VEGF） in peripheral blood and joint fluid of collagen-induced arthritis ...Objective： To observe the effect of electroacupuncture （EA） on tumor necrosis factor-α （TNF-α） and vascular endothelial growth factor （VEGF） in peripheral blood and joint fluid of collagen-induced arthritis （CIA） rats. Methods： After CIA rat models were successfully established, the rats were given corresponding intervention treatments. The levels of TNF-α and VEGF in peripheral blood and joint fluid were measured. Results： The levels of TNF-α and VEGF levels in peripheral blood and joint fluid of CIA rats were higher than those of normal group （P〈0.05）; EA treatment significantly decreased the index levels, of significant difference compared with those before treatment （P〈0.05）, as well as the model group （P〈0.05）. Conclusion： EA can significantly decrease the TNF-α and VEGF levels in peripheral blood and joint fluid of CIA rats, and helps restrain further development of the disease.展开更多
基金Supported by National Natural Science Foundation of China, No. 30171135 National Key Program for Basic Research of China, No. 2001CCA00700
文摘AIM: To determine the role of acupuncture therapy in treating experimental gastric ulcer in rats.METHODS: Twenty-eight adult male Sprague-Dawley rats were randomly divided into four groups (pre-acupuncture group; acupuncture group; paradistance-acupuncture group;and control group), and pre-acupuncture, paradistanceacupuncture, and control groups received 5 μL acetic acid (200 mL/L HAc) injection after a same course of electroacupuncture (EA) treatment (4 Hz, 0.6 mA, 0.45 ms,45 min for 4 d). The rats in these three groups recovered within 4 d. The acupuncture group received EA therapy for 4 d, after HAc injection. The stomach was dissected to compare the pathological structures of ulcer. Also c-Fos activation in the nuclei of solitary tract (NTS) was observed under microscope after regular immunohistochemistry staining of brain stem sections.RESULTS: The number of ulcers was different among the four groups, especially between control group and paradistance-acupuncture group or pre-acupuncture group. In the latter group, the number of ulcers was much less. The gastric ulcer area was consistent with the histopathological results, indicating that pre-acupuncture had an obvious therapeutic effect on gastric ulcers.Acupuncture had a very modest effect and paradistanceacupuncture had no effect on gastric ulcers. No therapeutic effect was found in the control group. Fos-Li neurons in NTS induced by noxious gastric ulcer showed a significant difference between pre-acupuncture and control groups.CONCLUSION: Acupuncture before ulceration can obviously alleviate ulcer. The production of c-Fos proves that the vagus nerve mediates the induction of c-Fos in nuclei of solitary tract following experimental ulceration,suggesting that parasympathetic afferents promote the process of noxious visceral stimulation.
文摘Objective: To explore the possible mechanisms of "JIN San Zhen" (JIN’s 3 acupoints groups) in treatment of mental retardation. Methods: A total of 38 female mice were randomly divided into normal control (n=10), sham operation (n=10), model (n=8) and acupuncture (n=10) groups. "Si Shen Zhen" [4 points around "Baihui" (GV 20): 0.3 cm anterior, posterior, left and right to GV 20 respectively], and "Zhi San Zhen" ["Shenting" (GV 24) and bilateral "Benshen" (GB 13)] of "JIN San Zhen" series are used. The memory ability, AChE positive fiber density and mono amines transmitters of the brain are used as the indexes. Results: After 8 days’ treatment, cerebral ischemia induced mental retardation (decline of learning memory ability) was improved significantly shown by Y maze test (P<0.05-0.01), simultaneously, AChE positive fiber density of the frontal cerebral cortex and hippocampus, serotonie (5 HT), noradrenaline (NA) and dopamine (DA) contents of the brain increased significantly (P<0.05). Conclusion: Acupuncture induced increase of AChE, 5 HT, NA and DA in the brain may contribute to the effect of acupuncture in improving the memory capability of the RD mice.
文摘Objective.. To compare the mechanisms of analgesia induced by four kinds of acupunture therapies at the trypothalamic level in adjuvant arthritic rats. Methods： Forty-eight SD rats were randomized into normal, model, electroacupuncture （EA）, filiform needle （FN）, pricking blood-letting （BL） and point injection （PI） groups, with 8 cases in each. EA （20-100 Hz, 2-4 V and duration of 20 min）, FN, BL PI were respectively applied to “Kunlun” （昆仑 BL 60）. Arthritis model was established by injecting complete Freund＇s adjuvant （0.1 mL） into the rat＇s right foot pad. Behavioral reactions, pain threshold （latancy of tail flick to heat stimulation） and local swelling severity （foot volurne） were detected; the cordents of β-endorphin （β-EP） and α- drenocortiootropin （ACTH） were assayed with radioimmunoassay; and the expression of pro-opi-omelanocortin （POMC） mRNA in hypot were determined with hybridization method. Results： The pain threshold was significantly enhanced by all the four kinds of acupuncture therapies, and the effects of EA and PI were more obvious （P〈0.05, P〈0.01）. The severity of local swelling was markedly alleviated by all the four kinds of acupuncture therapies （ P〈 0.01 ） without significant differences among them （P 〉 0.05）. The content of β-EP in the hypothalamus was obviously elevated by EA and FN （P〈 0.05, P 〈 0.01 ）, and the effect of EA was more obvious, but no marked effect of BL or PI on β-EP was found （ P 〉0.05）. The content of ACTH in hypothalamus was considerably elevated by PI （P〈0.05）, but not by the other three therapies. The expression of PCMCmRNA in hypothalamus was significantly strengthened by EA and FN （P〈0. 01）, but not by the other two therapies. Conclusion：EA, flliform needle, blood-letting and point-injection all can produce analgesic effect in adjuvant arthritis rats, the effect of EA and filiform needie may be related to their resultant increase of hypothalamic β-EP, and that of point-injectiON related to the increase of hypothalamic ACTH level.
基金Supported by the Science Research Fund of Hubei Province, No.WZ1539
文摘AIM: To study the nervous-pathways of Fengch'ih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP). METHODS: Fifty Wistar rats were randomly divided into 1, 2,3,4, and 5 d groups, and every group had 10 animals. HRP (30% aqueous solution) was injected into a Fengch'ih. Serial, transverse or capital, 40 urn sections of the cervical spinal ganglia, cervical and thoracic spinal cord segment and brain were cut on a cryotome. Sections were incubated for HRP histochemistry according to the tetramethylbenzidine (TMB). Part of the sections were counterstained with neutral red. RESULTS: After 1 d of survival times, many labeled cell bodies were found in 1-4 cervical spinal ganglia, anterior horn of 1-4 cervical spinal cord, ventromedial division of facial nucleus, accessory facial nucleus ipsilaterally. With increasing survival times, the intensity of labeled cells were slightly decreased. CONCLUSION: Fengch'ih may bring into full play its effect by correlation of posterior ear branch of facial nerve and anterior branch of 2-3 cervical nerve with 1-4 cervical the anterior horn of the spinal cord, ventromedial division of facial nucleus, accessory facial nucleus.
文摘PURPOSE: To observe the effects of acupuncture on the pituitary-thyroid axis in rabbits with fracture. METHOD: 36 male rabbits with a 3-mm width experimental defect in the middle segment of the left radius were randomly divided into the treatment group and control group, and the changes in serum T3, T4 and TSH contents in different weeks were investigated. RESULTS: Serum T3, T4 and TSH contents in the treatment group all were higher than those in the control group, with statistically significant differences in the contents of T4 and TSH in the second week and T3 content in the fourth week (P
文摘Objective： To verify two hypotheses： 1 ） Acupuncture can increase the re-distribution of tetramethylpyrazine （ligustrazine） hydrochloride （TMPH） to the target organs in adjuvant arthritic rats. 2） Cyclic Adenosine-3＇, 5＇-menophesphete （cAMP） is one of the guidance cues involved in this event. Methods： A total of 40 Wistar rats were randomized into blank control （BC）, model （MO）, medication （ME）, Acupuncture （Acu） and Acu＋ ME groups, with 8 cases in each group. Arthritis model was established by subcutaneous injection of complete Freund＇ s adjuvant （0.1 mL） into the rat＇ s foot pad. Electrcacupuncture （ 15 Hz, 0.2-0.3V） was applied to bilateral “Daling”（大陵 PC 7）, “Taichong” （太冲 LR 3） and “Xiguan” （膝关 LR 7） for 20 min, and the contents of TMPH in the heart, liver, kidney and the foot （affected focus） tissues and the contents of cAMP in the heart and liver tissues were determined with high performance liquid chromatography and radioimmunoassay techniques respectively. Results： Oornpared with medication group, the TMPH contents in the heart, liver, kidney and the affected foot tissues in Acu-ME group was obviously higher （ P〈0.05, P〈0.01 ）. In comparison with ME and Acu groups, the cAMP contents in the heart and liver in Acu＋ ME group was also significantly higher （P〈 0.05, P 〈 0.01 ）. No significant differences were found between ME and Acu ＋ ME groups in TMPH content of kidney and between Acu＋ ME and BC groups in cAMP contents in both liver and heart tissues. Conclusion： Acupuncture may promote the target-tropism distribution of ligustrazine, and cAMP may play an important role in it.
文摘Objective： This study aimed to prove acupuncture therapy exerted an obvious clinical efficacy in the treatment of peripheral facial neuritis in acute phase. Methods： 60 clinically collected cases were chosen in the study and randomly divided into control group and treatment group. In control group, dexamethasone, vitamin B, vitamin B 12, and mecobalamine were applied in acute phase, and then acupuncture therapy was combined in remission. In treatment group, western medicine combining with acupuncture therapy was used in acute phase, and three courses of treatment were totally applied. Results： The final conditions of two groups were good, and the grouping was balanced, so the comparability between them was good （P〉0.05）; the curative effects of two groups were significantly different： the effect of treatment group was better than that of control group （P〈0.01）. Conclusion： The acute phase was necessarily grasped to apply acupuncture therapy to the treatment of peripheral facial neuritis, and the curative effect would be more significant if acupuncture therapy was more early used.
基金The study has been supported by Fund of National Administrative Bureau of TCM for the Young, Grant No. 93C025
文摘Effects of electroacupuncture (EA) and routine acupuncture with twirling reinforcing and reducing manipulation of the needle (RA) both at Zusanli point (St 36) on volume of microcirculatory blood flow in the cerebral pia mater were observed by fenestration of the cranial bone and laser Doppler microcirculatory blood flow analyser. Results showed that both RA and EA could increase the volume of microcirculatory blood flow in the cerebral pia mater; and that the increase in the EA group was superior to that in RA group. This suggests that a moderate and effective stimulation is a key to the production of a regulative effect on the organism.
基金supported by a grant from Science and Technical Activities of State Administration of Traditional Chinese Medicine of the People's Republic of China for the Returnees(2005 LHR10)Science and Technical Specialization of State Administration of Traditional Chinese Medicine of the People's Republic of China(06-07 JP54)
文摘Objective:To probe into the most effective site,extra-ordinary point,acupoint and channel for regulating reproductive endocrine function by means of the study on acupuncture activating the gonadotropin-releasing hormone neurons(GnRH) in hypothalamus.Methods:Female SD rats of reproductive age were used,and the in vivo study on GnRH neurons in hypothalamus was made with mimic sexual stimulation and feedback regulation.The neuron-activating effects of the acupoints on the channels pertaining to the zang-and fu-organs related with reproductive endocrine and the extra-ordinary points in different regions were studied using the discharge of the neuron as index,and then the neurons were labeled with horseradish peroxidase(HRP) .Results:Acupuncture was given at two acupoints each on the three yin channels of foot,the three yang channels of foot,the Conception Vessel and the Governor Vessel.The order of the mean increasing percentage in the hypothalamic GnRH neuron electric activity was:the Gallbladder Channel>the Spleen Channel>the Stomach Channel/the Bladder Channel/the Conception Vessel>the Liver Channel>the Kidney Channel>the Governor Vessel;for different acupoints,it was:Guanyuan(CV 4) >Sanyinjiao(SP 6) >Zusanli(ST 36) >Daimai(GB 26) /Yanglingquan(GB 34) >Shenshu(BL 23) >Weizhong(BL 40) >Yaoyangguan(GV 3) /Liangmen(ST 21) /Fujie(SP 14) >Qimen(LR 14) /Yingu(KI 10) >Tangzhong(CV 17) /Zhiyang(GV 9) ;for different positions,it was:the lower abdominal part/the lower limb part>the thoracodorsal part;for the extra-ordinary points,it was:Zigong(EX-CA1) >Dannang(EX-LE6) /Yaoyan(EX-B7) >Baichongwo(EX-LE3) /Qianzheng>Jingbi/Bizhong/Taiyang(EX-HN5) >Erbai(EX-UE2) /Dingchun;and for the distribution sites of the extra-ordinary points:lower abdominal region>the lower limb region>the craniofacial region>the upper limb region/the thoracodorsal region.Conclusion:For regulating the reproductive endocrine function,the acupoints located at the same neural segment with the reproductive organ should be selected as the main points,and it is necessary to combine with syndrome differentiation of the viscera and channels.
基金supported by Seientific and Technologieal Development Plan Project of Suzhou (SS0528)
文摘Objective： To observe the effect of electroacupuncture （EA） on tumor necrosis factor-α （TNF-α） and vascular endothelial growth factor （VEGF） in peripheral blood and joint fluid of collagen-induced arthritis （CIA） rats. Methods： After CIA rat models were successfully established, the rats were given corresponding intervention treatments. The levels of TNF-α and VEGF in peripheral blood and joint fluid were measured. Results： The levels of TNF-α and VEGF levels in peripheral blood and joint fluid of CIA rats were higher than those of normal group （P〈0.05）; EA treatment significantly decreased the index levels, of significant difference compared with those before treatment （P〈0.05）, as well as the model group （P〈0.05）. Conclusion： EA can significantly decrease the TNF-α and VEGF levels in peripheral blood and joint fluid of CIA rats, and helps restrain further development of the disease.
