Path prediction of flexible needles based on the Fokker-Planck equation and disjunctive Kriging model is proposed to improve accuracy and consider the nonlinearity and anisotropy of soft tissues.The stochastic differe...Path prediction of flexible needles based on the Fokker-Planck equation and disjunctive Kriging model is proposed to improve accuracy and consider the nonlinearity and anisotropy of soft tissues.The stochastic differential equation is developed into the Fokker-Planck equation with Gaussian noise,and the position and orientation probability density function of flexible needles are then optimized by the stochastic differential equation.The probability density function obtains the mean and covariance of flexible needle movement and helps plan puncture paths by combining with the probabilistic path algorithm.The weight coefficients of the ordinary Kriging are extended to nonlinear functions to optimize the planned puncture path,and the Hermite expansion is used to calculate nonlinear parameter values of the disjunctive Kriging optimization model.Finally,simulation experiments are performed.Detailed comparison results under different path planning maps show that the kinematics model can plan optimal puncture paths under clinical requirements with an error far less than 2 mm.It can effectively optimize the path prediction model and help improve the target rate of soft tissue puncture with flexible needles through data analysis and processing of the mean value and covariance parameters derived by the probability density and disjunctive Kriging algorithms.展开更多
In the present paper, the authors make a simple comment on the influence of the theory of five elements of TCM on the acupuncturology from ① its influence on the meridian-collateral theory and acupoint system; ② its...In the present paper, the authors make a simple comment on the influence of the theory of five elements of TCM on the acupuncturology from ① its influence on the meridian-collateral theory and acupoint system; ② its influence on acu-moxibustion therapeutics including the application of mother and son points for reinforcing and reducing, the application of the five element theory to the midnight-noon ebb-flow needling, and guiding point selection in different seasons; and ③ its influence on acupuncture techniques.展开更多
Objective To explore the effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats. Methods Sixty SPF Wistar rats were randomly divided into four groups, including a normal c...Objective To explore the effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats. Methods Sixty SPF Wistar rats were randomly divided into four groups, including a normal control group (control group), a sham operation group, a model group and a treatment group, with 15 rats in each group. In the model group and treatment group, insomnia model were established by injecting orexin into lateral ventricle of rats. In the sham operation group, artificial cerebrospinal fluid was injected into lateral ventricle of rats, and the rats in control group were not treated. On the basis of modeling, the rats in treatment group were treated by acupuncture combined with medicated thread moxibustion of Zhuang medicine which was conducted once a day, and the treatment lasted for 14 days. The expression of orexin mRNA and orexin content were detected, and the changes of associated receptors, including orexin 1 receptor (OX I R) and orexin 2 receptor (OX 2 R) were observed. Results The expression of orexin mRNA in model group (6.54 ± 1.21) significantly increased when compared with control group (0.66±0.12, P〈0.01), and orexin content in model group [(i 156.35±126.88) pg/mL] significantly increased when compared with control group [(427.12_± 32.54) pg/mL, P〈O.OI]. The expression of orexin mRNA in treatment group (0.87 ± 0.26) significantly reduced when compared with the model group (P〈0.01), and orexin content in the treatment group [(585.23 ± 89.21) pg/mL] significantly reduced when compared with the model group (P〈O.01). The percentage of OX I R positive cells in the model group (0.64±0.15) significantly increased when compared with the control group (0.21±0.03, P〈0.01), and the percentage of OX 2 R positive cells in the model group (0.76±0.18) significantly increased when compared with the control group (0.24±0.01, P〈O.01). The percentage of OX 1 R positive cells in the treatment group (0.36_±0.08) significantly reduced when compared with the model group (P〈0.01). The percentage of OX 2 R positive cells in treatment group (0.25_± 0.03) significantly reduced when compared with model group (P〈0.01). Conclusion It might be an effective link of acupuncture combined with medicated thread moxibustion of Zhuang medicine in treatment of insomnia that the treatment could inhibit the awakening promotion effect of orexin by affecting orexin, OX I R and OX 2 R.展开更多
Objective:To observe the effect of acupuncture on the expression of mitochondrial proteome in hippocampus of senescence-accelerated mouse prone g (SAMPg) mice models with Alzheimer disease (AD),and to explore the...Objective:To observe the effect of acupuncture on the expression of mitochondrial proteome in hippocampus of senescence-accelerated mouse prone g (SAMPg) mice models with Alzheimer disease (AD),and to explore the possible protective mechanism of acupuncture on mitochondria.Methods:Sixty 6-month-old male SAMP8 mice were randomly divided into an acupuncture at acupoint group,an acupuncture at non-acupoint group and a model group,20 mice in each group.The 20 male senescence-accelerated mouse/resistance 1 (SAMR1) mice of the same age were used as a normal control group.Shenshu (BL 23),Baihui (GV 20),Xuehai (SP 10) and Geshu (BL 17) were selected for acupuncture intervention in acupuncture at acupoint group.After an 8-week intervention,mitochondrial tissues were extracted from the hippocampus.Differentially expressed proteins were identified by subcellular organelle proteomics.Western blot was used to verify the expressions of some related proteins in hippocampal mitochondria.Results:Compared with the model group,there were 13 differentially expressed protein spots in the acupuncture at acupoint group,of which,9 were up-regulated,including neurofilament light polypeptide (NFL),actin (cytoplasmic 1,database ID:ACTB),tubulin beta-2A chain (TBB2A),tropomodulin-2 (TMOD2),pyruvate dehydrogenase E1 component subunit beta (PDHE1-β),NADH-ubiquinone oxidoreductase 75 kDa subunit (database ID:NDUS1),heat shock cognate 71 kDa protein (HSC71),pyruvate dehydrogenase E1 component subunit alpha (PDHE1-α) and ATP synthase beta subunit (ATP-β);4 were down-regulated,including glial fibrillary acidic protein (GFAP),pyruvate dehydrogenase phosphatase 1 (PDP1),mitochondrial-processing peptidase subunit alpha (MMP-α) and adenosine kinase (ADK).According to the information provided in the protein database,most of the differentially expressed proteins involve the regulation of mitochondrial function and structure.The expression levels of NFL and TBB2A in the normal control group and the acupuncture at acupoint group were significantly higher than those in the acupuncture at non-acupoint group (P〈0.05).ATP-β and NDUS1 expression levels were significantly higher in the acupuncture at acupoint group than those in the acupuncture at non-acupoint group (P〈0.05);there was no significant difference between the acupuncture at non-acupoint group and the model group (P〉0.05).Conclusion:Acupuncture may achieve the potential therapeutic effect on AD by regulating the structure and functional proteins of hippocampal mitochondria.展开更多
Objective: To observe the clinical effect of thread-embedding therapy in treating insomnia due to heart-spleen deficiency. Methods: Sixty patients were randomized into an acupuncture group and a thread-embedding gro...Objective: To observe the clinical effect of thread-embedding therapy in treating insomnia due to heart-spleen deficiency. Methods: Sixty patients were randomized into an acupuncture group and a thread-embedding group, 30 in each. The acupuncture group was managed by acupuncture therapy, and the thread-embedding group was treated by thread-embedding therapy. Pittsburgh Sleep Quality Index (PSQI) was adopted for evaluation before and after treatments. Results: The total effective rate was 86.7% in the acupuncture group versus 96.7% in the thread-embedding group. After treatment, the PSQI scores were significantly different from those before treatment in both groups (P〈0.01); the scores in both two groups were mai'kedly different from each other after treatment (P〈0.01). Conclusion: Both acupuncture and thread-embedding therapies can effectively improve sleep quality of the patients, while thread-embedding therapy is comparatively less time-consuming, more convenient and with longer stimulation.展开更多
OBJECTIVE:To observe the effects of acupuncture on motility of jejunum and distal colon at different acupoints on normal Kunming mice.METHODS:The jejunal and colorectal motor activity of forty Kunming mice were record...OBJECTIVE:To observe the effects of acupuncture on motility of jejunum and distal colon at different acupoints on normal Kunming mice.METHODS:The jejunal and colorectal motor activity of forty Kunming mice were recorded by the intra-intestinal capsule.The capsules contained with distilled water were placed into the cavities of jejunum and distal colon.The changes of bowel were compared with the background activity recorded before any stimulation.The chosen acupoints were as follows:Zusanli(ST 36) on the lower legs,Tianshu(ST 25) on the mid-lower abdomen.RESULTS:Manual acupuncture(MA) at Zusanli(ST36) significantly promoted the motility of jejunum and distal colon(manifested as increased change rate of average amplitude and increased change rate of mean area under the contractile curve) of normal Kunming mice(P < 0.01).MA atTianshu(ST25) significantly inhibited the motility of jejunum(manifested as decreased change rate of average amplitude,decreased change rate of mean area under the contractile curve,and the reduced frequency) of normal Kunming mice(P < 0.01),while significantly increased the distal colonic motility(manifested as increased amplitude and increased mean area under the contractile curve) of normal Kunming mice(P < 0.01).CONCLUSION:MA at specific acupoints[Zusanli(ST 36),Tianshu(ST 25)]has different effects on the motility of jejunum and distal colon,which might involve the segmental innervations of the related nerve.展开更多
Summary: Fouty-five cases of temporomandibular joint dysfunction syndrome were diagnosed by Xray, excluding the organic diseases. The acupoints Xiaguan (ST 7), Jiache (ST 6), Yifeng (TE 17) and Hegu (LI 4) on...Summary: Fouty-five cases of temporomandibular joint dysfunction syndrome were diagnosed by Xray, excluding the organic diseases. The acupoints Xiaguan (ST 7), Jiache (ST 6), Yifeng (TE 17) and Hegu (LI 4) on the affected side were punctured first, after removal of needles, 5-7 layers of cotton cloth was placed on the above acupoints, then moxibustion with moxa-roll made of Chinese medicine was performed. Finally Dian-finger pressing, An-pressing and Rou stationary circular pressing with finger belly of the thumb were applied to the local region around the mandible joint for 3-5 min. After 1-2 courses of treatments, 45 cases all got effect.展开更多
基金The National Natural Science Foundation of China(No.61903175,62163024,62163026)the Academic and Technical Leaders Foundation of Major Disciplines of Jiangxi Province under Grant(No.20204BCJ23006).
文摘Path prediction of flexible needles based on the Fokker-Planck equation and disjunctive Kriging model is proposed to improve accuracy and consider the nonlinearity and anisotropy of soft tissues.The stochastic differential equation is developed into the Fokker-Planck equation with Gaussian noise,and the position and orientation probability density function of flexible needles are then optimized by the stochastic differential equation.The probability density function obtains the mean and covariance of flexible needle movement and helps plan puncture paths by combining with the probabilistic path algorithm.The weight coefficients of the ordinary Kriging are extended to nonlinear functions to optimize the planned puncture path,and the Hermite expansion is used to calculate nonlinear parameter values of the disjunctive Kriging optimization model.Finally,simulation experiments are performed.Detailed comparison results under different path planning maps show that the kinematics model can plan optimal puncture paths under clinical requirements with an error far less than 2 mm.It can effectively optimize the path prediction model and help improve the target rate of soft tissue puncture with flexible needles through data analysis and processing of the mean value and covariance parameters derived by the probability density and disjunctive Kriging algorithms.
文摘In the present paper, the authors make a simple comment on the influence of the theory of five elements of TCM on the acupuncturology from ① its influence on the meridian-collateral theory and acupoint system; ② its influence on acu-moxibustion therapeutics including the application of mother and son points for reinforcing and reducing, the application of the five element theory to the midnight-noon ebb-flow needling, and guiding point selection in different seasons; and ③ its influence on acupuncture techniques.
基金Supported by National Natural Science Foundation of China:81360579
文摘Objective To explore the effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats. Methods Sixty SPF Wistar rats were randomly divided into four groups, including a normal control group (control group), a sham operation group, a model group and a treatment group, with 15 rats in each group. In the model group and treatment group, insomnia model were established by injecting orexin into lateral ventricle of rats. In the sham operation group, artificial cerebrospinal fluid was injected into lateral ventricle of rats, and the rats in control group were not treated. On the basis of modeling, the rats in treatment group were treated by acupuncture combined with medicated thread moxibustion of Zhuang medicine which was conducted once a day, and the treatment lasted for 14 days. The expression of orexin mRNA and orexin content were detected, and the changes of associated receptors, including orexin 1 receptor (OX I R) and orexin 2 receptor (OX 2 R) were observed. Results The expression of orexin mRNA in model group (6.54 ± 1.21) significantly increased when compared with control group (0.66±0.12, P〈0.01), and orexin content in model group [(i 156.35±126.88) pg/mL] significantly increased when compared with control group [(427.12_± 32.54) pg/mL, P〈O.OI]. The expression of orexin mRNA in treatment group (0.87 ± 0.26) significantly reduced when compared with the model group (P〈0.01), and orexin content in the treatment group [(585.23 ± 89.21) pg/mL] significantly reduced when compared with the model group (P〈O.01). The percentage of OX I R positive cells in the model group (0.64±0.15) significantly increased when compared with the control group (0.21±0.03, P〈0.01), and the percentage of OX 2 R positive cells in the model group (0.76±0.18) significantly increased when compared with the control group (0.24±0.01, P〈O.01). The percentage of OX 1 R positive cells in the treatment group (0.36_±0.08) significantly reduced when compared with the model group (P〈0.01). The percentage of OX 2 R positive cells in treatment group (0.25_± 0.03) significantly reduced when compared with model group (P〈0.01). Conclusion It might be an effective link of acupuncture combined with medicated thread moxibustion of Zhuang medicine in treatment of insomnia that the treatment could inhibit the awakening promotion effect of orexin by affecting orexin, OX I R and OX 2 R.
文摘Objective:To observe the effect of acupuncture on the expression of mitochondrial proteome in hippocampus of senescence-accelerated mouse prone g (SAMPg) mice models with Alzheimer disease (AD),and to explore the possible protective mechanism of acupuncture on mitochondria.Methods:Sixty 6-month-old male SAMP8 mice were randomly divided into an acupuncture at acupoint group,an acupuncture at non-acupoint group and a model group,20 mice in each group.The 20 male senescence-accelerated mouse/resistance 1 (SAMR1) mice of the same age were used as a normal control group.Shenshu (BL 23),Baihui (GV 20),Xuehai (SP 10) and Geshu (BL 17) were selected for acupuncture intervention in acupuncture at acupoint group.After an 8-week intervention,mitochondrial tissues were extracted from the hippocampus.Differentially expressed proteins were identified by subcellular organelle proteomics.Western blot was used to verify the expressions of some related proteins in hippocampal mitochondria.Results:Compared with the model group,there were 13 differentially expressed protein spots in the acupuncture at acupoint group,of which,9 were up-regulated,including neurofilament light polypeptide (NFL),actin (cytoplasmic 1,database ID:ACTB),tubulin beta-2A chain (TBB2A),tropomodulin-2 (TMOD2),pyruvate dehydrogenase E1 component subunit beta (PDHE1-β),NADH-ubiquinone oxidoreductase 75 kDa subunit (database ID:NDUS1),heat shock cognate 71 kDa protein (HSC71),pyruvate dehydrogenase E1 component subunit alpha (PDHE1-α) and ATP synthase beta subunit (ATP-β);4 were down-regulated,including glial fibrillary acidic protein (GFAP),pyruvate dehydrogenase phosphatase 1 (PDP1),mitochondrial-processing peptidase subunit alpha (MMP-α) and adenosine kinase (ADK).According to the information provided in the protein database,most of the differentially expressed proteins involve the regulation of mitochondrial function and structure.The expression levels of NFL and TBB2A in the normal control group and the acupuncture at acupoint group were significantly higher than those in the acupuncture at non-acupoint group (P〈0.05).ATP-β and NDUS1 expression levels were significantly higher in the acupuncture at acupoint group than those in the acupuncture at non-acupoint group (P〈0.05);there was no significant difference between the acupuncture at non-acupoint group and the model group (P〉0.05).Conclusion:Acupuncture may achieve the potential therapeutic effect on AD by regulating the structure and functional proteins of hippocampal mitochondria.
文摘Objective: To observe the clinical effect of thread-embedding therapy in treating insomnia due to heart-spleen deficiency. Methods: Sixty patients were randomized into an acupuncture group and a thread-embedding group, 30 in each. The acupuncture group was managed by acupuncture therapy, and the thread-embedding group was treated by thread-embedding therapy. Pittsburgh Sleep Quality Index (PSQI) was adopted for evaluation before and after treatments. Results: The total effective rate was 86.7% in the acupuncture group versus 96.7% in the thread-embedding group. After treatment, the PSQI scores were significantly different from those before treatment in both groups (P〈0.01); the scores in both two groups were mai'kedly different from each other after treatment (P〈0.01). Conclusion: Both acupuncture and thread-embedding therapies can effectively improve sleep quality of the patients, while thread-embedding therapy is comparatively less time-consuming, more convenient and with longer stimulation.
基金Supported by National Basic Research Program of China:Mechanisms of Dual Effects of Acupuncture on Functional Intestinal Disease(973 Program,No.2011CB505200)the National Natural Science Foundation of China Research Grants:Investigations on Effect of Acupuncture In Functional Bowel Disorders Via CRF Signaling Pathway(No.81202763)
文摘OBJECTIVE:To observe the effects of acupuncture on motility of jejunum and distal colon at different acupoints on normal Kunming mice.METHODS:The jejunal and colorectal motor activity of forty Kunming mice were recorded by the intra-intestinal capsule.The capsules contained with distilled water were placed into the cavities of jejunum and distal colon.The changes of bowel were compared with the background activity recorded before any stimulation.The chosen acupoints were as follows:Zusanli(ST 36) on the lower legs,Tianshu(ST 25) on the mid-lower abdomen.RESULTS:Manual acupuncture(MA) at Zusanli(ST36) significantly promoted the motility of jejunum and distal colon(manifested as increased change rate of average amplitude and increased change rate of mean area under the contractile curve) of normal Kunming mice(P < 0.01).MA atTianshu(ST25) significantly inhibited the motility of jejunum(manifested as decreased change rate of average amplitude,decreased change rate of mean area under the contractile curve,and the reduced frequency) of normal Kunming mice(P < 0.01),while significantly increased the distal colonic motility(manifested as increased amplitude and increased mean area under the contractile curve) of normal Kunming mice(P < 0.01).CONCLUSION:MA at specific acupoints[Zusanli(ST 36),Tianshu(ST 25)]has different effects on the motility of jejunum and distal colon,which might involve the segmental innervations of the related nerve.
文摘Summary: Fouty-five cases of temporomandibular joint dysfunction syndrome were diagnosed by Xray, excluding the organic diseases. The acupoints Xiaguan (ST 7), Jiache (ST 6), Yifeng (TE 17) and Hegu (LI 4) on the affected side were punctured first, after removal of needles, 5-7 layers of cotton cloth was placed on the above acupoints, then moxibustion with moxa-roll made of Chinese medicine was performed. Finally Dian-finger pressing, An-pressing and Rou stationary circular pressing with finger belly of the thumb were applied to the local region around the mandible joint for 3-5 min. After 1-2 courses of treatments, 45 cases all got effect.