Objective To observe effects of acupuncture prestimulus on heat shock protein HSP 70,HSP 90 and apoptotic rate of hippocampal neuron in model rats with Alzheimer＇s disease（AD） in order to investigate the functional...Objective To observe effects of acupuncture prestimulus on heat shock protein HSP 70,HSP 90 and apoptotic rate of hippocampal neuron in model rats with Alzheimer＇s disease（AD） in order to investigate the functional mechanism in preventing and treating AD by acupuncture.Methods SD rats were randomly divided into control,sham operation,model and acupuncture prestimulus groups,with 10 rats in each group.In the pre-acupuncture group,electroacupuncture and moxibus on were given at ＂Bǎihuì＂（百会 GV 20） and ＂Shènshū＂（肾俞 BL 23） on both sides for three weeks,then Aβ1-42 was injected into hippocampus on both sides to prepare the model rats with AD. After AD＇s model was established,EA and moxibustion were given again for one week. Immunohistochemical analysis was carried out to determine the mean op cal density expression of HSP 70 and HSP 90 in CA 1 area of hippocampus in the rats,and flow cytometry was used to determine the apopto c rates of hippocampal neuron cells. Results A er acupuncture pres mulus for 4 weeks,the mean op cal density expression of HSP 70 and HSP 90 in CA 1 area of hippocampus in the rats significantly increased in comparison to that in the model group（P0.01）,and the apopto c rate of the cells significantly decreased in comparison to that in the model group（P0.01）. Conclusion Acupuncture pres mulus can inhibit cell apoptosis by inducing the expression of HSP 70 and HSP 90 in brain,and thus protect neurons.展开更多
基金Supported by National Natural Science Founda on of China:81173323,30901925Natural Science Founda on of Hubei Province:2007 ABA 084
文摘Objective To observe effects of acupuncture prestimulus on heat shock protein HSP 70,HSP 90 and apoptotic rate of hippocampal neuron in model rats with Alzheimer＇s disease（AD） in order to investigate the functional mechanism in preventing and treating AD by acupuncture.Methods SD rats were randomly divided into control,sham operation,model and acupuncture prestimulus groups,with 10 rats in each group.In the pre-acupuncture group,electroacupuncture and moxibus on were given at ＂Bǎihuì＂（百会 GV 20） and ＂Shènshū＂（肾俞 BL 23） on both sides for three weeks,then Aβ1-42 was injected into hippocampus on both sides to prepare the model rats with AD. After AD＇s model was established,EA and moxibustion were given again for one week. Immunohistochemical analysis was carried out to determine the mean op cal density expression of HSP 70 and HSP 90 in CA 1 area of hippocampus in the rats,and flow cytometry was used to determine the apopto c rates of hippocampal neuron cells. Results A er acupuncture pres mulus for 4 weeks,the mean op cal density expression of HSP 70 and HSP 90 in CA 1 area of hippocampus in the rats significantly increased in comparison to that in the model group（P0.01）,and the apopto c rate of the cells significantly decreased in comparison to that in the model group（P0.01）. Conclusion Acupuncture pres mulus can inhibit cell apoptosis by inducing the expression of HSP 70 and HSP 90 in brain,and thus protect neurons.
