The ruthenium multi-substituted polyoxotungstate,K_7[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O(S1),was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anionsβ-Na_(9)HSi W_...The ruthenium multi-substituted polyoxotungstate,K_7[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O(S1),was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anionsβ-Na_(9)HSi W_(9)O_(34)·12H_(2)O(S2)and Ru Cl_(3)·n H_(2)O(S3).Compound S1 was charac‐terized by elemental analysis,energy-dispersive X-ray spectroscopy(EDS),thermogravimetric analysis(TG),infrared spectroscopy(IR),uliraviolet visible absorption spectroscopy(UV/Vis)and X-ray photoelectron spectroscopy(XPS).The cytotoxicitycy of S1 was tested in C33A(human cervical cancer),DLD-1(human colon cancer),Hep G2(human liver cancer)and human normal embryonic lung fibroblasts cell(MRC-5).And the viability of these treated cells was evaluated by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro‐mide)assay.To explore the mode of cell death induced by S1,morphological study of DNA damage and apoptosis assays were conducted.These analyses revealed that S1 exerted its cytotoxic effect in a dose-dependent manner,primarily triggering apoptotic cell death.Cell cycle analysis by flow cytometry indicated that compound S1 caused cell cycle arrest and accumulated cells in S phase.展开更多
基金Supported by the National Natural Science Foundation of China (21701120)the Science and Technology Innovation Project of Colleges and Universities in Shanxi Province (2020L0334)the Innovation and Entrepreneurship Training Program for College Students in Shanxi Province(20240778)。
文摘The ruthenium multi-substituted polyoxotungstate,K_7[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O(S1),was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anionsβ-Na_(9)HSi W_(9)O_(34)·12H_(2)O(S2)and Ru Cl_(3)·n H_(2)O(S3).Compound S1 was charac‐terized by elemental analysis,energy-dispersive X-ray spectroscopy(EDS),thermogravimetric analysis(TG),infrared spectroscopy(IR),uliraviolet visible absorption spectroscopy(UV/Vis)and X-ray photoelectron spectroscopy(XPS).The cytotoxicitycy of S1 was tested in C33A(human cervical cancer),DLD-1(human colon cancer),Hep G2(human liver cancer)and human normal embryonic lung fibroblasts cell(MRC-5).And the viability of these treated cells was evaluated by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro‐mide)assay.To explore the mode of cell death induced by S1,morphological study of DNA damage and apoptosis assays were conducted.These analyses revealed that S1 exerted its cytotoxic effect in a dose-dependent manner,primarily triggering apoptotic cell death.Cell cycle analysis by flow cytometry indicated that compound S1 caused cell cycle arrest and accumulated cells in S phase.
