The role of second messenger pathways, cyclic AMP, calcium,and protein and protein kinase C(PKC) in the transcriptional regulation of c-fos proto-oncogene expression in rat Sertoli cells was investigated, c-fos expre...The role of second messenger pathways, cyclic AMP, calcium,and protein and protein kinase C(PKC) in the transcriptional regulation of c-fos proto-oncogene expression in rat Sertoli cells was investigated, c-fos expression wasmonitored by Northern blot analysis.Although the action of FSH on Sertolicells is considered to be mediated by cAMP, dibutyryl cAMP(db cAMP), apotent membrane permeable analog of cAMP,induced much less c-fos mRNA expression than FSH(<50%), suggesting that additional cAMPindependent mechanisms may mediate the effect of FSH on c-fos. Specificintracellular inhibitors of PKC decreased c-fos induction in response to FSHby more than 50%. Ionomycin, which increases intracellular free calciumconcentration, induced c-fos expression significantly. These datademonstrate that Sertoli cell c-fos mRNA expression in under multifactorial regulation by cAMP, calcium, and PKC.展开更多
文摘The role of second messenger pathways, cyclic AMP, calcium,and protein and protein kinase C(PKC) in the transcriptional regulation of c-fos proto-oncogene expression in rat Sertoli cells was investigated, c-fos expression wasmonitored by Northern blot analysis.Although the action of FSH on Sertolicells is considered to be mediated by cAMP, dibutyryl cAMP(db cAMP), apotent membrane permeable analog of cAMP,induced much less c-fos mRNA expression than FSH(<50%), suggesting that additional cAMPindependent mechanisms may mediate the effect of FSH on c-fos. Specificintracellular inhibitors of PKC decreased c-fos induction in response to FSHby more than 50%. Ionomycin, which increases intracellular free calciumconcentration, induced c-fos expression significantly. These datademonstrate that Sertoli cell c-fos mRNA expression in under multifactorial regulation by cAMP, calcium, and PKC.
