高能冲击波对肾脏钙离子腺苷三磷酸酶活性的影响及川芎嗪的保护作用

目的观察高能冲击波对肾脏钙离子腺苷三磷酸酶（Ca^2＋ -ATPase）活性的影响及川芎嗪的保护作用，探讨高能冲击波肾损伤机制。方法30只健康家兔制成单肾动物模型，按完全随机设计法分为对照组10只、高能冲击波组10只和川芎嗪组10只，对照组、高能冲击波组分别静脉注射等量生理盐水，高能冲击波冲击肾脏前3天，川芎嗪组静脉注射川芎嗪；冲击肾脏24h后，光学法检测肾细胞膜和线粒体膜Ca^2＋ -ATPase活性；采用原位缺口末端标记法和流式细胞术检测凋亡细胞；采用生化分析测定内生肌酐清除率。结果与对照组比较，高能冲击波组肾细胞膜和线粒体膜Ca^2＋ -ATPase活性均降低（P〈0．05，P〈0．01）、细胞凋亡率增加（P〈0．01）、内生肌酐清除率降低（P〈0．01）；川芎嗪组肾细胞膜Ca^2＋ -ATPase活性和内生肌酐清除率变化不明显（P〉0．05），线粒体膜Ca^2＋ -ATPase活性降低（P〈0．05）、细胞凋亡率增加（P〈0．05），但幅度明显小于高能冲击波组。结论高能冲击波冲击肾脏后肾脏Ca^2＋ -ATPase 活性降低是发生肾细胞凋亡导致肾功能损伤的重要机制，川芎嗪有改善肾功能的作用，与其阻制肾脏Ca^2＋ -ATPase 活性降低、抗细胞凋亡有关。

异丙酚和硫喷妥钠对离体大鼠心脏功能和细胞质膜Ca^(2+)-ATPase活性的影响

目的 观察不同浓度异丙酚或硫喷妥钠对离体灌流大鼠心脏收缩力和心肌细胞质膜Ca2 + ATPase活性的影响。方法 ( 1)以含不同浓度 ( 10 μmol/L～ 10 0 0 μmol/L)异丙酚或硫喷妥钠的克 汉二氏重碳酸盐缓冲液 (KHB)灌流离体大鼠心脏 ,测定心率 (HR)、左室收缩压 (LVSP)、左室收缩压上升最大速率 ( +dp/dtmax)和左室舒张压下降最大速率 ( -dp/dtmax)。 ( 2 )参照文献制备大鼠心肌细胞质膜 ,观察不同浓度异丙酚或硫喷妥钠对质膜Ca2 + ATPase活性的影响。结果 随着药液浓度的升高 ,异丙酚或硫喷妥钠使LVSP、+dp/dtmax、-dp/dtmax逐渐降低 ,在等摩尔浓度的抑制作用异丙酚 >硫喷妥钠 ,高浓度的异丙酚或硫喷妥钠还可以减慢心率。异丙酚对质膜Ca2 + ATPase活性的影响呈双相 ,低浓度 (≤ 2 0 μmol/L)使酶的活性升高 ,而≥ 5 0 μmol/L的异丙酚使质膜Ca2 + ATPase活性下降 ,与基础值比较有统计学意义 (P <0 0 5 )。硫喷妥钠只有在浓度≥ 10 0mmol/L时对Ca2 + AT Pase活性有抑制作用。结论 异丙酚或硫喷妥钠呈浓度依赖地抑制心肌收缩和舒张功能 ,异丙酚呈双相地影响心肌质膜Ca2 + ATPase的活性 。

一种菊酯类复合剂对黑胸散白蚁体内CarEs和Ca-ATPase活性的影响

以黑胸散白蚁ReticulitermeschinensisSnyder为试验材料 ,研究了一种白蚁防治复合剂中的主要成分对白蚁体内羧酸酯酶 (CarEs)和钙 腺苷三磷酸酶 (Ca ATPase)的影响。结果表明 :氯菊酯在终浓度为 1 6 6× 10 - 4mol·L- 1 以下时 ,对羧酸酯酶无明显抑制作用 ;八氯二丙醚和壬基酚聚氧乙烯醚在此浓度下都对羧酸酯酶表现出明显抑制作用 ,其IC50 分别为 7 114 8× 10 - 5mol·L- 1 和 7 3373× 10 - 4mol·L- 1 ;氯菊酯对Ca ATPase表现出较强抑制作用 ,IC50 为 5 11× 10 - 7mol·L- 1 。认为Ca AT Pase是黑胸散白蚁体内拟除虫菊酯类杀虫剂作用的主要靶标之一。

Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca^2＋ transport proteins and the effect of taurine on myocardial protection in rabbits

To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca 2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX , 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function , concentration of calcium in cardiomyocytes (Myo[Ca 2+ ] \%i\%), activity of SR Ca 2+ ATPase(SERCA2a), level of SERCA2a mRNA and Ca 2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca 2+ ATPase and level of SERCA2a mRNA decreased , while Myo[Ca 2+ ] \%i\% increased in DOX treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca 2+ ] \%i\% and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.

烧伤大鼠延迟性复苏对心肌组织Na+,K+-ATPase、Ca2+-ATPase活性的影响

目的：研究烧伤休克延迟复苏对心肌组织Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋－ＡＴＰａｓｅ活性及含水量的影响。方法：雄性ＳＤ大鼠造成３０％体表面积的Ⅲ度烧伤，分为烧伤不复苏（Ｂ）组、早期复苏（ＢＥ）组和延迟复苏（ＢＤ）组，伤后８ｈ取左心室肌组织，测定含水量，并匀浆后用比色法测定Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋－ＡＴＰａｓｅ活性。结果：Ｂ组心肌组织Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋－ＡＴＰａｓｅ活性均明显低于正常对照（Ｃ）组（Ｐ＜０．０１）；ＢＤ组２种酶活性均非常显著低于ＢＥ组（Ｐ＜０．０１），并明显低于Ｂ组（Ｐ＜０．０５）；Ｂ组心肌组织含水量非常明显低于ＢＥ组和ＢＤ组，ＢＤ组比ＢＥ组有增高趋势，但无显著差异。结论：烧伤休克延迟复苏严重损害心肌组织Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋－ＡＴＰａｓｅ活性。

实验性动脉粥样硬化兔血小板胞浆游离钙和钙泵的变化

实验观察了食饵性兔动脉粥样硬化形成过程中血小板胞浆游离钙［Ｃａ（２＋）］ｉ、血清钙、红细胞膜钙泵即Ｃａ（２＋）＋Ｍｇ（２＋）－ＡＴＰａｓｅ的活性，主动脉收缩性的变化，与对照组比较结果表明：（１）高脂兔血小板［Ｃａ（２＋）］ｉ水平增高，红细胞膜Ｃａ（２＋）－Ｍｇ（２＋）－ＡＴＰａｓｅ活性及主动脉收缩性降低；（２）主动脉组织光镜可见高脂兔主动脉中层有大量钙沉积；（３）扫描电镜可见血小板粘附于内皮细胞表面。

白藜芦醇对心肌缺血再灌注过程中线粒体的保护

目的:探讨白藜芦醇对心肌缺血再灌注损伤过程中Ca2+-ATPase及HSP70表达的影响。方法:健康雄性SD大鼠40只,采用随机数字量表法分为4组(假手术组、缺血再灌注组、缺血预适应组、白藜芦醇组),采用结扎冠状动脉前降支制备心肌缺血再灌注损伤模型,于结扎前15 min及再灌注前1 min经舌下静脉注射白藜芦醇10 mg·kg-1。提取心肌线粒体,紫外可见光分光光度计测定线粒体中SDH、Na+-K+-ATPase、Ca2+-ATPase活性;荧光分光光度计测定线粒体中游离钙、m PTP开放度及跨膜电位;RT-PCR及Western blot检测心肌组织HSP70、Ca2+-ATPase m RNA及蛋白质的表达。结果:白藜芦醇与缺血再灌注组及缺血预适应组相比,线粒体中SDH、Na+-K+-ATPase、Ca2+-ATPase的活性明显提高(P<0.05或P<0.01);线粒体内钙离子浓度明显下降(P<0.05或P<0.01)、m PTP开放程度减小(P<0.01)、线粒体膜电位增高(P<0.01);Ca2+-ATPase、HSP70在m RNA或蛋白水平上的表达均有所增加(P<0.05或P<0.01)。结论:白藜芦醇可能通过增加心肌组织中Ca2+-ATPase、HSP70 mRNA和蛋白的表达,提高线粒体Ca2+-ATPase的活性,产生对大鼠心肌缺血再灌注损伤时线粒体的保护作用。

Effect of Acupuncture on NO, NOS and Ca^(2+)-Mg^(2+)-ATPase Activity in Brain Tissue of Subacute Aging Rats Induced by D-galactose

Objective： To explore the mechanism of acupuncture in treating brain aging. Methods： Forty healthy Wistar rats were randomly divided into 4 groups with 10 rats in each group. The rats in the control group didn＇t received modeling and treatment. The rats in the model group, the Governor Vessel acupoints group （GVA group） and the body acupoints group （BA group） were induced as subacute aging rat model. The rats in the model group didn＇t take treatment. On the modeling day, the rats in the GVA group received acupuncture at Baihui （GV 20） and Dazhui （GV 14） while the rats in the BA group received acupuncture at Guanyuan （CV 3） and Zusanli （ST 36）. The contents of NO and NOS, as well as the activity of Ca^2＋-Mg^2＋-ATPase in the brain tissue of rats in each group were observed. Results： After modeling, the contents of NO and NOS of brain tissue increased significantly and the activity of Ca2^＋-Mg^2＋-ATPase decreased markedly. Acupuncture treatment could inhibit the increases of NO and NOS contents, improve the decrease of the activity of Ca^2＋-Mg^2＋-ATPase. The results of the GVA group were better than those of BA group （both P〈0.05）. Conclusion： Acupuncture improved the activity of Ca^2＋-Mg^2＋-ATPase and stabilized the NOS concentration. As a result, the NO content was balanced and the ohvsiological function of NO was fully induced for anti-a^in~, esoeciallv in the GVA group.