The molecular weight of bovine brain CaM, intracellular CaM and extracellularCaM of cauliflower have been determined for the first time by Matrix-Assisted Larer Desorp tion Ionization Time of Flight Mass Spectrometry(...The molecular weight of bovine brain CaM, intracellular CaM and extracellularCaM of cauliflower have been determined for the first time by Matrix-Assisted Larer Desorp tion Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS). The molecular weightof bovine brain CaM, 16 699. 9 Da determined by MALDI-TOF-MS, is closely ideatical with16 700 Da, calculated from its primary sequence, showing the high accuracy of MALDITOF-MS and the conf1rmation of previously determined sequence. Furthermore, the molecular weight of intracellular CaM and extracellular CaM determined by MAlDI-TOF-MS, 17025 Da and 15 582 Da, are in agreement with 17 500 Da and 15 000 Da determined by SDSPAGE-This agreement shows the high accuracy of MALDI-TOF-MS. In addition, themolecular weight of CaMs with this technique can be determined rapidly(less than 10 min)and sensitively.展开更多
文摘The molecular weight of bovine brain CaM, intracellular CaM and extracellularCaM of cauliflower have been determined for the first time by Matrix-Assisted Larer Desorp tion Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS). The molecular weightof bovine brain CaM, 16 699. 9 Da determined by MALDI-TOF-MS, is closely ideatical with16 700 Da, calculated from its primary sequence, showing the high accuracy of MALDITOF-MS and the conf1rmation of previously determined sequence. Furthermore, the molecular weight of intracellular CaM and extracellular CaM determined by MAlDI-TOF-MS, 17025 Da and 15 582 Da, are in agreement with 17 500 Da and 15 000 Da determined by SDSPAGE-This agreement shows the high accuracy of MALDI-TOF-MS. In addition, themolecular weight of CaMs with this technique can be determined rapidly(less than 10 min)and sensitively.
