The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli

The Clock gene,a key molecule in circadian systems,is widely distributed in the animal kingdom. We isolated a 936-bp partial c DNA sequence of the C lock gene( Pva- clock) from the darkbarbel catfish P elteobagrus vachelli that exhibited high identity with C lock genes of other species of fish and animals(65%–88%). The putative domains included a basic helix-loop-helix(b HLH) domain and two period-ARNT-single-minded(PAS) domains,which were also similar to those in other species of fish and animals. P va- Clock was primarily expressed in the brain,and was detected in all of the peripheral tissues sampled. Additionally,the pattern of P va- Clock expression over a 24-h period exhibited a circadian rhythm in the brain,liver and intestine,with the acrophase at zeitgeber time 21:35,23:00,and 23:23,respectively. Our results provide insight into the function of the molecular C lock of P. vachelli.

The influence of hepatitis B virus X protein on the clock genes in liver cells and its significance

Objective： The aim of this study was to investigate the influence of hepatitis B virus X protein （HBx） on the clock genes in LO2 cells and its significance. Methods： A cell line LO2-HBx, Stably transfected with HBx gene, was established. The levels of mRNA and protein expression of CLOCK and BMAL1 were detected by real-time PCR and western blot. Resuits： The expression of CLOCK mRNA and protein were increased in cell line LO2-HBx （P 〈 0.05）, while the expression of BMAL1 mRNA and protein were decreased in cell line LO2-HBx （P 〈 0.05）. Conclusion： The expressions of core clock gene CLOCK and BMAL1 have been changed by HBx, which breaks down the previous circadian rhythm of liver cells. This maybe one of the reasons leads to the formation of liver cancer.

Teleportation of N-qubit W State without Bell-State Measurement via Selective Resonant Interaction in Cavity QED

We present a scheme in which the N-atom W state is teleported by employing the selective interactionof a cavity field with a driven three-level atom in the A configuration and detecting a single atom in one of the groundstates.The long-lived W state is teleported from atom A to atom B when the atoms B and A are sent through acavity successively and atom A is then detected.The advantage is that the present one does not involve the Bell-statemeasurement and is robust against the atomic spontaneous emission.

Circatidal rhythms of locomotion in the American horseshoe crab Limulus polyphemus: Underlying mechanisms and cues that influence them

While eye sensitivity in the American horseshoe crab Limulus polyphemus has long been known to be under the control of an endogenous circadian clock, only recently has horseshoe crab locomotion been shown to be controlled by a separate clock system. In the laboratory, this system drives clear activity rhythms throughout much of the year, not just during the mating season when horseshoe crabs express clear tidal rhythms in the field. Water temperature is a key factor influencing the expression of these rhythms： at 17~C tidal rhythms are expressed by most animals, while at I l^C expression of circatidal rhythms is rarely seen, and at 4~C rhythms are suppressed. Neither long （16：8 Light：Dark） nor short （8：16） photoperiods modify this behavior at any of these temperatures. Synchronization of these circatidal rhythms can be most readily effeeted by water pressure cycles both in situ and in the lab, while temperature and current cycles play lesser, but possibly contributory, roles. Interestingly, Light：Dark cycles appear to have synchronizing as well as ＂masking＂ effects in some individuals. Evidence that each of two daily bouts of activity are independent suggests that the Limulus circatidal rhythm of locomotion is driven by two （circalunidian） clocks, each with a period of 24.8h. While the anatomical locations of either the circadian clock, that drives fluctuations in visual sensitivity, or the circatidal clock, that controls tidal rhythms of locomotion, are currently unknown, preliminary molecular analyses have shown that a 71 kD protein that reacts with antibodies directed against the Drosophila PERIOD （PER） protein is found in both the pro- tocerebrum and the subesophageal ganglion

Numerical Study of a Novel Procedure for Installing the Tower and Rotor Nacelle Assembly of Offshore Wind Turbines Based on the Inverted Pendulum Principle

Current installation costs of offshore wind turbines(OWTs) are high and profit margins in the offshore wind energy sector are low, it is thus necessary to develop installation methods that are more efficient and practical. This paper presents a numerical study(based on a global response analysis of marine operations) of a novel procedure for installing the tower and Rotor Nacelle Assemblies(RNAs) on bottom-fixed foundations of OWTs. The installation procedure is based on the inverted pendulum principle. A cargo barge is used to transport the OWT assembly in a horizontal position to the site, and a medium-size Heavy Lift Vessel(HLV) is then employed to lift and up-end the OWT assembly using a special upending frame. The main advantage of this novel procedure is that the need for a huge HLV(in terms of lifting height and capacity) is eliminated. This novel method requires that the cargo barge is in the leeward side of the HLV(which can be positioned with the best heading) during the entire installation. This is to benefit from shielding effects of the HLV on the motions of the cargo barge, so the foundations need to be installed with a specific heading based on wave direction statistics of the site and a typical installation season. Following a systematic approach based on numerical simulations of actual operations, potential critical installation activities, corresponding critical events, and limiting(response) parameters are identified. In addition, operational limits for some of the limiting parameters are established in terms of allowable limits of sea states. Following a preliminary assessment of these operational limits, the duration of the entire operation, the equipment used, and weather-and water depth-sensitivity, this novel procedure is demonstrated to be viable.

鸟中有凤，鱼中有鲲

楚襄王问宋玉说：“先生大概有不检点的行为吧？为什么士人百姓对你非议得如此厉害呢？”宋玉回答说：“是的，的确如此，确实有这样的事。希望大王宽恕我的罪过，让我把话说完。”

Effects of the interplanetary magnetic field clock angle on the shape of bow shock

Using the global magnetohydrodynamics(MHD) simulation model, we investigated the effects of the interplanetary magnetic field(IMF) clock angle on the shape of bow shock, including its rotational asymmetry and subsolar point. For general northward IMF( z component Bz > 0), the rotational symmetry of the bow shock is broken by the effects of fast magnetosonic Mach number(Mms), and the cross-sectional line of the bow shock is an ellipse with the semi-major axis along the direction perpendicular to the IMF. The ratio or D-value between semi-major and semi-minor axis can be used to illustrate the extent of asymmetry of the bow shock. On the basis of the multiple parameters fitting, we obtain the changing relationship of both semi-axes with the clock angle and the distance away from the Earth. For general southward IMF(Bz < 0), the cross sectional line of the bow shock is highly asymmetrical under the multiple effects of magnetopause and Mms. The effects of IMF clock angle on subsolar point depend mainly on the changing subsolar point of magnetopause as an obstacle. The distance of subsolar point of bow shock from the Earth increases with the increasing IMF clock angle for Bz > 0, and decreases with the increasing IMF clock angle for Bz < 0.