Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira that infects humans and a wide range of animals. By combining computational prediction and high-accuracy tandem m...Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira that infects humans and a wide range of animals. By combining computational prediction and high-accuracy tandem mass spectra, we revised the genome annotation of Leptospira interrogans serovar Lai, a free-living pathogenic spirochete responsible for leptospirosis, providing substantial peptide evidence for novel genes and new gene boundaries. Subsequently, we presented a high-coverage proteome analysis of protein expression and multiple posttranslational modifications （PTMs）. Approximately 64.3% of the predicted L. interrogans proteins were cataloged by detecting 2 540 proteins. Meanwhile, a profile of multiple PTMs was concurrently established, containing in total 32 phosphorylated, 46 acetylated and 155 methylated proteins. The PTM systems in the serovar Lai show unique features. Unique eukaryotic-like features of L. interrogans protein modifications were demonstrated in both phosphorylation and arginine methylation. This systematic analysis provides not only comprehensive information of high-coverage protein expression and multiple modifications in prokaryotes but also a view suggesting that the evolutionarily primitive L. interrogans shares significant similarities in protein modification systems with eukaryotes.展开更多
In Indonesia, most transmission of leptospirosis occurs through the rat under conditions of high rainfall or flooding. Sampang is endemic leptospirosis, during March-May 2013, there were 55 patients with 8 of them die...In Indonesia, most transmission of leptospirosis occurs through the rat under conditions of high rainfall or flooding. Sampang is endemic leptospirosis, during March-May 2013, there were 55 patients with 8 of them died. The purpose of this study was to analyze risk factors for outbreaks leptospirosis by climatic factors. Study is cross-sectional design of variable causes or risks that occur due to the object of research are measured simultaneously. The results showed that the distribution of events is based on epidemiological characteristics of leptospirosis in districts Sampang both rainy and dry season. Analysis of climatic factors show support to the continuation of leptospirosis whose temperatures ranged from 29.35 ~C-30.62 ~C, humidity range between 63.4%-80.5%. Rainfall ranges from 183-190 ram. Incidence of leptospirosis in Sampang shows the distribution of leptospirosis cases from 18 subdistricts, 14 subdistriets are at risk of the occurrence of leptospirosis. Results obtained rat trapping species caught in the neighborhood residential home patients were mice （Rattus tanezumi and Rattus novergicus） and the identification of the leptospira in rat urine sewers rat positive standing of the potential risk of leptospirosis in the community. The conclusions are climatic conditions, rainfall remarkable effecting on the incidence of leptospirosis.展开更多
文摘Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira that infects humans and a wide range of animals. By combining computational prediction and high-accuracy tandem mass spectra, we revised the genome annotation of Leptospira interrogans serovar Lai, a free-living pathogenic spirochete responsible for leptospirosis, providing substantial peptide evidence for novel genes and new gene boundaries. Subsequently, we presented a high-coverage proteome analysis of protein expression and multiple posttranslational modifications （PTMs）. Approximately 64.3% of the predicted L. interrogans proteins were cataloged by detecting 2 540 proteins. Meanwhile, a profile of multiple PTMs was concurrently established, containing in total 32 phosphorylated, 46 acetylated and 155 methylated proteins. The PTM systems in the serovar Lai show unique features. Unique eukaryotic-like features of L. interrogans protein modifications were demonstrated in both phosphorylation and arginine methylation. This systematic analysis provides not only comprehensive information of high-coverage protein expression and multiple modifications in prokaryotes but also a view suggesting that the evolutionarily primitive L. interrogans shares significant similarities in protein modification systems with eukaryotes.
文摘In Indonesia, most transmission of leptospirosis occurs through the rat under conditions of high rainfall or flooding. Sampang is endemic leptospirosis, during March-May 2013, there were 55 patients with 8 of them died. The purpose of this study was to analyze risk factors for outbreaks leptospirosis by climatic factors. Study is cross-sectional design of variable causes or risks that occur due to the object of research are measured simultaneously. The results showed that the distribution of events is based on epidemiological characteristics of leptospirosis in districts Sampang both rainy and dry season. Analysis of climatic factors show support to the continuation of leptospirosis whose temperatures ranged from 29.35 ~C-30.62 ~C, humidity range between 63.4%-80.5%. Rainfall ranges from 183-190 ram. Incidence of leptospirosis in Sampang shows the distribution of leptospirosis cases from 18 subdistricts, 14 subdistriets are at risk of the occurrence of leptospirosis. Results obtained rat trapping species caught in the neighborhood residential home patients were mice （Rattus tanezumi and Rattus novergicus） and the identification of the leptospira in rat urine sewers rat positive standing of the potential risk of leptospirosis in the community. The conclusions are climatic conditions, rainfall remarkable effecting on the incidence of leptospirosis.
