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苦豆子、铁棒锤生物碱对菜青虫的毒力测定 被引量:13
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作者 刘锦霞 张锐 +1 位作者 晁开 刘长仲 《甘肃科学学报》 2003年第2期38-41,共4页
 苦豆子、铁棒锤生物碱对菜青虫毒力测定的结果表明,苦豆子、铁棒锤生物碱对菜青虫均有较好的胃毒作用,用致死中量表示苦豆子生物碱LD50=30.07μg/g,铁棒锤生物碱LD50=38.83μg/g.室内触杀作用试验和田间防效试验表明,苦豆子生物碱有...  苦豆子、铁棒锤生物碱对菜青虫毒力测定的结果表明,苦豆子、铁棒锤生物碱对菜青虫均有较好的胃毒作用,用致死中量表示苦豆子生物碱LD50=30.07μg/g,铁棒锤生物碱LD50=38.83μg/g.室内触杀作用试验和田间防效试验表明,苦豆子生物碱有效剂量为2000μg/mL、600μg/mL和铁棒锤生物碱有效剂量为2000μg/mL时,对菜青虫有很好的防治效果,在施药后72h,校正防治效果可达80%~90%,且仍呈逐渐增大趋势(活虫数逐渐减少趋势). 展开更多
关键词 苦豆子生物碱 铁棒锤生物碱 菜青虫 毒力 防效
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正交试验优化铁棒锤生物碱提取工艺 被引量:6
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作者 杜弢 陈红刚 +1 位作者 林丽 魏学明 《中国实验方剂学杂志》 CAS 北大核心 2011年第3期20-22,共3页
目的:优选铁棒锤生物碱的最佳提取工艺。方法:选择溶剂、提取次数、洗涤次数为考察因素,以总生物碱的含量为考察指标,采用正交试验法优选铁棒锤生物碱的最佳提取工艺。结果:铁棒锤生物碱的最佳提取工艺为浸提次数3次,提取溶剂为乙醚-三... 目的:优选铁棒锤生物碱的最佳提取工艺。方法:选择溶剂、提取次数、洗涤次数为考察因素,以总生物碱的含量为考察指标,采用正交试验法优选铁棒锤生物碱的最佳提取工艺。结果:铁棒锤生物碱的最佳提取工艺为浸提次数3次,提取溶剂为乙醚-三氯甲烷(3∶1),洗涤1次。结论:优选得到的提取工艺稳定性好、得率高、简便可行。 展开更多
关键词 正交试验 铁棒锤生物碱 提取工艺 酸碱滴定法
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Abnormal Secondary Growth and Histochemical Localization of Alkaloids in Root System of Aconitum flavum Hand.-Mazz.
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作者 祁建钊 郭继元 徐青 《Agricultural Science & Technology》 CAS 2011年第9期1313-1317,共5页
[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistr... [Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a "U"-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a "U" shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root. 展开更多
关键词 Aconitum flavum Hand. - Mazz. Root system Abnormal secondary growth ALKALOIDS Histochemical localization
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