目的探讨磁共振非对称回波的最小二乘估算法迭代水脂分离(iterative decomposition of water and fat with echo asymmetry and least squares estimation quantification,IDEAL-IQ)序列在甲胎蛋白(alpha fetoprotein,AFP)阴性肝细胞癌(...目的探讨磁共振非对称回波的最小二乘估算法迭代水脂分离(iterative decomposition of water and fat with echo asymmetry and least squares estimation quantification,IDEAL-IQ)序列在甲胎蛋白(alpha fetoprotein,AFP)阴性肝细胞癌(hepatocellular carcinoma,HCC)和肝脏局灶性结节性增生(focal nodular hyperplasia,FNH)诊断和鉴别诊断中的价值。材料与方法回顾性分析AFP阴性HCC 28例和FNH 15例患者资料,所有患者术前均行MRI肝脏常规扫描及IDEAL-IQ序列扫描,在后处理工作站测量病灶及背景肝组织弛豫率值(R2;)、脂肪分数(fat fraction,FF)值,统计学分析比较FF值、R2;值之间的差异,采用受试者工作特征(receiver operating characteristic,ROC)曲线分析比较相关参数在两组病变中的诊断效能。结果HCC组和FNH组FF;值、R2^(*)_(肝脏)/R2^(*)_(病灶)分别为8.28%±5.76%、2.98±1.08和2.56%±1.25%、1.90±0.50,两者之间差异具有统计学意义(P<0.05);两组病灶R2^(*)_(病灶)值、背景肝组织FF_(肝脏)值分别为(21.15±5.80)Hz、4.02%±2.54%和(22.79±6.07)Hz、4.23%±3.32%,差异无统计学意义(P>0.05)。FF_(病灶)值及R2^(*)_(肝脏)/R2^(*)_(病灶)曲线下面积(area under the curve,AUC)分别为0.923(P<0.001)、0.883(P=0.56)。结论FF;值及R2^(*)_(肝脏)/R2^(*)_(病灶)对于两者均具有鉴别诊断价值,FF;值AUC大于R2^(*)_(肝脏)/R2^(*)_(病灶)。展开更多
The presence of actin in eukaryotic nuclei and chromosomes, and especially in higher plant nuclei and chromosomes, has not been well established. We detected actin in meristematic cells of Allium cepa with indirect im...The presence of actin in eukaryotic nuclei and chromosomes, and especially in higher plant nuclei and chromosomes, has not been well established. We detected actin in meristematic cells of Allium cepa with indirect immunofluorescence technique and observed bright fluorescence in the intact nuclei and chromosomes, indicating that actin is present in the nuclei and chromosomes of the higher plant. We labeled sections of the meristematic cells of A. cepa with immunogold technique, gold particles were found over the whole nuclei and a number of gold particles were concentrated in condensed chromatin and nucleoli, confirming the results of the immunofluoresence observations. We treated the nuclei and chromosomes of A.cepa with DNase I and 2M NaCl and obtained DNA- and histone-depleted nuclei and chromosomes. Indirect immunofluorescence tests showed that the DNA- and histonedepleted nuclei and chromosomes reacted positively with the anti-actin antibodies. These results demonstrate that actin exists not only in intact nuclei and chromosomes,but also in DNA- and histone-depleted nuclei and chrmosomes of the plant. In addition, our immuno-fluorescence tests indicate that tropomyosin is present in the nuclei and chromosomes of A. cepa.展开更多
文摘目的探讨磁共振非对称回波的最小二乘估算法迭代水脂分离(iterative decomposition of water and fat with echo asymmetry and least squares estimation quantification,IDEAL-IQ)序列在甲胎蛋白(alpha fetoprotein,AFP)阴性肝细胞癌(hepatocellular carcinoma,HCC)和肝脏局灶性结节性增生(focal nodular hyperplasia,FNH)诊断和鉴别诊断中的价值。材料与方法回顾性分析AFP阴性HCC 28例和FNH 15例患者资料,所有患者术前均行MRI肝脏常规扫描及IDEAL-IQ序列扫描,在后处理工作站测量病灶及背景肝组织弛豫率值(R2;)、脂肪分数(fat fraction,FF)值,统计学分析比较FF值、R2;值之间的差异,采用受试者工作特征(receiver operating characteristic,ROC)曲线分析比较相关参数在两组病变中的诊断效能。结果HCC组和FNH组FF;值、R2^(*)_(肝脏)/R2^(*)_(病灶)分别为8.28%±5.76%、2.98±1.08和2.56%±1.25%、1.90±0.50,两者之间差异具有统计学意义(P<0.05);两组病灶R2^(*)_(病灶)值、背景肝组织FF_(肝脏)值分别为(21.15±5.80)Hz、4.02%±2.54%和(22.79±6.07)Hz、4.23%±3.32%,差异无统计学意义(P>0.05)。FF_(病灶)值及R2^(*)_(肝脏)/R2^(*)_(病灶)曲线下面积(area under the curve,AUC)分别为0.923(P<0.001)、0.883(P=0.56)。结论FF;值及R2^(*)_(肝脏)/R2^(*)_(病灶)对于两者均具有鉴别诊断价值,FF;值AUC大于R2^(*)_(肝脏)/R2^(*)_(病灶)。
文摘The presence of actin in eukaryotic nuclei and chromosomes, and especially in higher plant nuclei and chromosomes, has not been well established. We detected actin in meristematic cells of Allium cepa with indirect immunofluorescence technique and observed bright fluorescence in the intact nuclei and chromosomes, indicating that actin is present in the nuclei and chromosomes of the higher plant. We labeled sections of the meristematic cells of A. cepa with immunogold technique, gold particles were found over the whole nuclei and a number of gold particles were concentrated in condensed chromatin and nucleoli, confirming the results of the immunofluoresence observations. We treated the nuclei and chromosomes of A.cepa with DNase I and 2M NaCl and obtained DNA- and histone-depleted nuclei and chromosomes. Indirect immunofluorescence tests showed that the DNA- and histonedepleted nuclei and chromosomes reacted positively with the anti-actin antibodies. These results demonstrate that actin exists not only in intact nuclei and chromosomes,but also in DNA- and histone-depleted nuclei and chrmosomes of the plant. In addition, our immuno-fluorescence tests indicate that tropomyosin is present in the nuclei and chromosomes of A. cepa.
