目的探讨妊娠中期妊娠期糖尿病(GDM)孕妇血清铁(SI)、血清铁蛋白(SF)、血糖等水平,分析SI、SF与GDM发生的相关性。方法 232例行常规产前检查的妊娠24~28周孕妇,其中健康孕妇为对照组, GDM孕妇为观察组,各116例。对两组SI、SF、血红蛋白(...目的探讨妊娠中期妊娠期糖尿病(GDM)孕妇血清铁(SI)、血清铁蛋白(SF)、血糖等水平,分析SI、SF与GDM发生的相关性。方法 232例行常规产前检查的妊娠24~28周孕妇,其中健康孕妇为对照组, GDM孕妇为观察组,各116例。对两组SI、SF、血红蛋白(Hb)、血糖等指标进行检测,并分析其相关性。结果观察组中家族高血压史87.9%,家族糖尿病史78.4%,均高于对照组的64.7%、75.9%,差异有统计学意义(P<0.05)。观察组体质量指数(BMI)为(31.78±1.46)kg/m^(2),高于对照组的(21.22±1.38)kg/m^(2),差异有统计学意义(P<0.05)。观察组SI、SF、空腹血糖(FPG)、餐后1 h血糖(1 h PG)、餐后2 h血糖(2 h PG)、甘油三酯(TG)水平均高于对照组,差异有统计学意义(P<0.05)。两组总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平比较,差异无统计学意义(P>0.05)。SI、SF与FPG、1 h PG、2 h PG均呈正相关(P<0.05)。单因素Logistic回归分析显示,SI、SF是GDM发病的危险因素(P<0.05)。结论 GDM孕妇SI、SF水平相比正常孕妇明显更高,并且随着其SI、SF水平的升高, GDM发病风险越来越大,可见妊娠中期孕妇体内铁存储水平升高和GDM发病存在密切的相关性,推断SI、SF为GDM发生的危险因素。展开更多
AIM： To investigate effects of iron on oxidative stress, heme oxygenase-1 （HMOX1） and hepatitis C viral （HCV） expression in human hepatoma ceils stably expressing HCV proteins. METHODS： Effects of iron on oxidat...AIM： To investigate effects of iron on oxidative stress, heme oxygenase-1 （HMOX1） and hepatitis C viral （HCV） expression in human hepatoma ceils stably expressing HCV proteins. METHODS： Effects of iron on oxidative stress, HMOX1, and HCV expression were assessed in CON1 cells. Measurements included mRNA by quantitative reverse transcription-polymerase chain reaction, and protein levels by Western blots. RESULTS： Iron, in the form of ferric nitrilotriacetate,increased oxidative stress and upegulated HMOX1 gene expression. Iron did not affect mRNA or protein levels of Bach1, a repressor of HMOXl. Silencing the up-regulation of HMOXl nuclear factor-erythroid 2-related factor 2 （Nrf2） by Nrf2-siRNA decreased FeNTA-mediated up-regulation of HMOXl mRNA levels. These iron effects were completely blocked by deferoxamine （DFO）. Iron also significantly decreased levels of HCV core mRNA and protein by 80%-90%, nonstructural 5A mRNA by 90% and protein by about 50% in the Con1 full length HCV replicon cells, whereas DFO increased them. CONCLUSION： Excess iron up-regulates HMOXl and down-regulates HCV gene expression in hepatoma cells. This probably mitigates liver injury caused by combined iron overload and HCV infection.展开更多
African indigenous vegetables have many nutritional and health benefits that have not been well researched and fully exploited. The objective of this study was to determine iron and protein contents of seven priority ...African indigenous vegetables have many nutritional and health benefits that have not been well researched and fully exploited. The objective of this study was to determine iron and protein contents of seven priority African indigenous vegetables found in Eastern Africa. The vegetables were planted at two sites, Maseno University, Maseno in western Kenya and Jomo Kenyatta University of Agriculture and Technology （JKUAT）, Juja in Central Kenya between 2006 and 2008. These vegetables were organically grown and edible parts of each of the vegetable harvested during vegetative growth stages just before onset of flowering and analysed for iron and protein contents. Nightshade and cowpea had high levels of both iron and protein. Pumpkin leaves and amaranths had high iron content while spiderplant and slenderleaf had high protein levels. Both iron and protein levels differed significantly between the seven vegetables at both sites. Nightshade and cowpea contained iron and protein levels that would provide 100% of the recommended daily allowance （RDA） iron and 50% of recommended daily allowance protein for optimal human growth and health. These results help to demonstrate the nutritional value of African indigenous vegetables and their potential use in nutrition intervention programs.展开更多
文摘目的探讨妊娠中期妊娠期糖尿病(GDM)孕妇血清铁(SI)、血清铁蛋白(SF)、血糖等水平,分析SI、SF与GDM发生的相关性。方法 232例行常规产前检查的妊娠24~28周孕妇,其中健康孕妇为对照组, GDM孕妇为观察组,各116例。对两组SI、SF、血红蛋白(Hb)、血糖等指标进行检测,并分析其相关性。结果观察组中家族高血压史87.9%,家族糖尿病史78.4%,均高于对照组的64.7%、75.9%,差异有统计学意义(P<0.05)。观察组体质量指数(BMI)为(31.78±1.46)kg/m^(2),高于对照组的(21.22±1.38)kg/m^(2),差异有统计学意义(P<0.05)。观察组SI、SF、空腹血糖(FPG)、餐后1 h血糖(1 h PG)、餐后2 h血糖(2 h PG)、甘油三酯(TG)水平均高于对照组,差异有统计学意义(P<0.05)。两组总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平比较,差异无统计学意义(P>0.05)。SI、SF与FPG、1 h PG、2 h PG均呈正相关(P<0.05)。单因素Logistic回归分析显示,SI、SF是GDM发病的危险因素(P<0.05)。结论 GDM孕妇SI、SF水平相比正常孕妇明显更高,并且随着其SI、SF水平的升高, GDM发病风险越来越大,可见妊娠中期孕妇体内铁存储水平升高和GDM发病存在密切的相关性,推断SI、SF为GDM发生的危险因素。
基金Supported by Grant(DK RO1 38825) and contracts(DK NO129236 and UO1 DK 06193)from the National Institutes of Health(NIDDK)
文摘AIM： To investigate effects of iron on oxidative stress, heme oxygenase-1 （HMOX1） and hepatitis C viral （HCV） expression in human hepatoma ceils stably expressing HCV proteins. METHODS： Effects of iron on oxidative stress, HMOX1, and HCV expression were assessed in CON1 cells. Measurements included mRNA by quantitative reverse transcription-polymerase chain reaction, and protein levels by Western blots. RESULTS： Iron, in the form of ferric nitrilotriacetate,increased oxidative stress and upegulated HMOX1 gene expression. Iron did not affect mRNA or protein levels of Bach1, a repressor of HMOXl. Silencing the up-regulation of HMOXl nuclear factor-erythroid 2-related factor 2 （Nrf2） by Nrf2-siRNA decreased FeNTA-mediated up-regulation of HMOXl mRNA levels. These iron effects were completely blocked by deferoxamine （DFO）. Iron also significantly decreased levels of HCV core mRNA and protein by 80%-90%, nonstructural 5A mRNA by 90% and protein by about 50% in the Con1 full length HCV replicon cells, whereas DFO increased them. CONCLUSION： Excess iron up-regulates HMOXl and down-regulates HCV gene expression in hepatoma cells. This probably mitigates liver injury caused by combined iron overload and HCV infection.
文摘African indigenous vegetables have many nutritional and health benefits that have not been well researched and fully exploited. The objective of this study was to determine iron and protein contents of seven priority African indigenous vegetables found in Eastern Africa. The vegetables were planted at two sites, Maseno University, Maseno in western Kenya and Jomo Kenyatta University of Agriculture and Technology （JKUAT）, Juja in Central Kenya between 2006 and 2008. These vegetables were organically grown and edible parts of each of the vegetable harvested during vegetative growth stages just before onset of flowering and analysed for iron and protein contents. Nightshade and cowpea had high levels of both iron and protein. Pumpkin leaves and amaranths had high iron content while spiderplant and slenderleaf had high protein levels. Both iron and protein levels differed significantly between the seven vegetables at both sites. Nightshade and cowpea contained iron and protein levels that would provide 100% of the recommended daily allowance （RDA） iron and 50% of recommended daily allowance protein for optimal human growth and health. These results help to demonstrate the nutritional value of African indigenous vegetables and their potential use in nutrition intervention programs.
