[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expr...[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. [Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. [ Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. Plant expression vector was screened out through connection, transformation and enzyme digestion identification. [ Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains.展开更多
Protein phosphorylation is one of the most common post-translational modification processes that play an essential role in regulating protein functionality.The Helicoverpa armigera single nucleopolyhedrovirus (HearNPV...Protein phosphorylation is one of the most common post-translational modification processes that play an essential role in regulating protein functionality.The Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) orf2-encoded nucleocapsid protein HA2 participates in orchestration of virus-induced actin polymerization through its WCA domain,in which phosphorylation status are supposed to be critical in respect to actin polymerization.In the present study,two putative phosphorylation sites (232Thr and 250Ser) and a highly conserved Serine (245Ser) on the WCA domain of HA2 were mutated,and their phenotypes were characterized by reintroducing the mutated HA2 into the HearNPV genome.Viral infectivity assays demonstrated that only the recombinant HearNPV bearing HA2 mutation at 245Ser can produce infectious virions,both 232Thr and 250Ser mutations were lethal to the virus.However,actin polymerization assay demonstrated that all the three viruses bearing HA2 mutations were still capable of initiating actin polymerization in the host nucleus,which indicated the putative phosphorylation sites on HA2 may contribute to HearNPV replication through another unidentified pathway.展开更多
Experimental results are presented for superfluid (He II) flow through porous plug liquid-vapor phase separators. Tests have been performed on seven porous plugs with different thicknesses or different permeabilities....Experimental results are presented for superfluid (He II) flow through porous plug liquid-vapor phase separators. Tests have been performed on seven porous plugs with different thicknesses or different permeabilities. The temperature was measured from 1.5K to 1.9K. Two flow regions were observed in small and large pressure and temperature differences regions respectively. The experimental data are compared with theoretical predictions. The performance and applicability of the basic theory are discussed. Hysteresis of the flow rate is also observed and discussed.展开更多
A simple and efficient system for Suzuki cross-coupling reactions was developed using a ligandless catalyst of Pd nanoclusters generated in situ from Pd(acac)2. The cross-coupling reactions proceeded under mild reacti...A simple and efficient system for Suzuki cross-coupling reactions was developed using a ligandless catalyst of Pd nanoclusters generated in situ from Pd(acac)2. The cross-coupling reactions proceeded under mild reaction conditions with a high reaction rate(5 min) to give various biaryls in high yields. The system also exhibited catalytic potential for Heck reaction between aryl bromides and styrene.展开更多
基金Supported by the National Natural Science Foundation Program(30160010)~~
文摘[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. [Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. [ Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. Plant expression vector was screened out through connection, transformation and enzyme digestion identification. [ Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains.
基金National Nature Science Foundations of China (31030027,30770085 and 30800044)
文摘Protein phosphorylation is one of the most common post-translational modification processes that play an essential role in regulating protein functionality.The Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) orf2-encoded nucleocapsid protein HA2 participates in orchestration of virus-induced actin polymerization through its WCA domain,in which phosphorylation status are supposed to be critical in respect to actin polymerization.In the present study,two putative phosphorylation sites (232Thr and 250Ser) and a highly conserved Serine (245Ser) on the WCA domain of HA2 were mutated,and their phenotypes were characterized by reintroducing the mutated HA2 into the HearNPV genome.Viral infectivity assays demonstrated that only the recombinant HearNPV bearing HA2 mutation at 245Ser can produce infectious virions,both 232Thr and 250Ser mutations were lethal to the virus.However,actin polymerization assay demonstrated that all the three viruses bearing HA2 mutations were still capable of initiating actin polymerization in the host nucleus,which indicated the putative phosphorylation sites on HA2 may contribute to HearNPV replication through another unidentified pathway.
文摘Experimental results are presented for superfluid (He II) flow through porous plug liquid-vapor phase separators. Tests have been performed on seven porous plugs with different thicknesses or different permeabilities. The temperature was measured from 1.5K to 1.9K. Two flow regions were observed in small and large pressure and temperature differences regions respectively. The experimental data are compared with theoretical predictions. The performance and applicability of the basic theory are discussed. Hysteresis of the flow rate is also observed and discussed.
基金financial support from the National Natural Science Foundation of China (21003092)the Key Project of Chinese Ministry of Education (211064)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘A simple and efficient system for Suzuki cross-coupling reactions was developed using a ligandless catalyst of Pd nanoclusters generated in situ from Pd(acac)2. The cross-coupling reactions proceeded under mild reaction conditions with a high reaction rate(5 min) to give various biaryls in high yields. The system also exhibited catalytic potential for Heck reaction between aryl bromides and styrene.
