铈氨基酸配合物对Oliogo DNA的切断

Cyclic voltammetry(CV)and ultravioletvisible spectroscopy(UV) were used to study cerium amino acid complexes.The amino acids are Phenylananine,Histidine,Serine,Threonine and Glutamicacid.These amino acids can coordinate with cerium ion(Ⅳ) to form complexes at near neutral condition(pH=7).At the same time,the denaturing ployacrylamidegel electrophoresissilver dye method was used to analyze the hydrolysis of 26mers oligodeoxyribonucleotide by cerium amino acid complexes around the neutral condition(pH=7).The results show that these cerium amino acid complexes can cleavage DNA at different degree.We also analyze the possible mechanism of the different hydrolysis effects.

铈氨基酸配合物的UV和CV方法

利用紫外光谱法和循环伏安法研究几种Ce^(4+)离子氨基酸配合物。结果表明几种氨基酸均能在pH为6的弱酸性条件下与Ce^(4+)形成配合物,不同的配体与Ce^(4+)形成的配合物的还原电位均比Ce^(4+)的更负。配体与Ce^(4+)的配比不同,形成配合物循环伏安曲线和紫外光谱有差异。