This study aimed to investigate the effects of copper and manganese on hemocyte apoptosis and the antagonism of iron and zinc in Oreochromis niloticus The heavy metal contents in fish blood and feed were determined by...This study aimed to investigate the effects of copper and manganese on hemocyte apoptosis and the antagonism of iron and zinc in Oreochromis niloticus The heavy metal contents in fish blood and feed were determined by atomic absorption spectrophotometry, and the hemocyte apoptosis was determined by flow cytometry. A total of 360 tilapias were selected, and they were divided randomly and evenly into 12 groups. In the challenge groups, the tilapias were fed with con stant-level copper sulfate（0, 200 mg/kg） and manganese sulfate（0, 120 mg/kg）; in the antagonism groups, the tilapias were fed with constant-level zinc sulfate（20320 mg/kg） and iron sulfate（150, 350 mg/kg）. After 20-week aquaculture, the hemocyte apoptosis rates in the copper and manganese groups were significantly increased; with the increased addition levels of iron and zinc, the hemocyte apopto sis rates in the iron and zinc groups were significantly reduced, but they were stil higher than that in the control group. In conclusion, excessive copper and manganese can cause apoptosis in hemocytes of O. niloticus. However, the toxic effects of copper and manganese can be antagonized by iron and zinc.展开更多
基金Supported by Scientific Operating Expenses of Hainan Province(11-201410-0009)~~
文摘This study aimed to investigate the effects of copper and manganese on hemocyte apoptosis and the antagonism of iron and zinc in Oreochromis niloticus The heavy metal contents in fish blood and feed were determined by atomic absorption spectrophotometry, and the hemocyte apoptosis was determined by flow cytometry. A total of 360 tilapias were selected, and they were divided randomly and evenly into 12 groups. In the challenge groups, the tilapias were fed with con stant-level copper sulfate（0, 200 mg/kg） and manganese sulfate（0, 120 mg/kg）; in the antagonism groups, the tilapias were fed with constant-level zinc sulfate（20320 mg/kg） and iron sulfate（150, 350 mg/kg）. After 20-week aquaculture, the hemocyte apoptosis rates in the copper and manganese groups were significantly increased; with the increased addition levels of iron and zinc, the hemocyte apopto sis rates in the iron and zinc groups were significantly reduced, but they were stil higher than that in the control group. In conclusion, excessive copper and manganese can cause apoptosis in hemocytes of O. niloticus. However, the toxic effects of copper and manganese can be antagonized by iron and zinc.
