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铝对大鼠神经干细胞向神经元细胞分化的影响 被引量:4
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作者 胡琼 王取南 +1 位作者 李嘉嘉 王岩 《安徽医科大学学报》 CAS 北大核心 2005年第3期210-213,共4页
目的探讨铝对大鼠神经干细胞向神经元样细胞分化的影响。方法取孕12~12.5dSD大鼠胚胎的大脑皮层细胞体外培养,建立神经干细胞模型。用Nestin免疫细胞化学法鉴定神经干细胞,在细胞对数增长期加入不同浓度的AlCl3(400、200、100μmol/L)... 目的探讨铝对大鼠神经干细胞向神经元样细胞分化的影响。方法取孕12~12.5dSD大鼠胚胎的大脑皮层细胞体外培养,建立神经干细胞模型。用Nestin免疫细胞化学法鉴定神经干细胞,在细胞对数增长期加入不同浓度的AlCl3(400、200、100μmol/L),接触24h后,用DMEM(含1%B27)培养基培养7d,免疫细胞化学染色比较各组MAP2阳性细胞率。用生物图像处理系统(包括NikonE800u显微镜,Spot2冷彩色数码摄像系统,MetaMorph图像分析软件)分析神经元树突分枝的数量和MAP2阳性细胞光密度(OD)值。结果与对照组相比,100μmol/LAlCl3组神经干细胞分化为MAP2阳性细胞率(%)、神经元树突分枝和MAP2阳性细胞光密度值均没有明显差异(P=0.082,P=0.117,P=0.086),但200μmol/L和400μmol/LAlCl3组与对照组相比均有明显降低(P<0.01),并且有良好的剂量-反应关系。结论AlCl3不仅能够抑制大脑神经干细胞向神经元样细胞分化,还减少了神经元分枝和延缓了其成熟。这可能在铝导致的神经发育毒性作用中起重要的作用。 展开更多
关键词 铝/毒性 大鼠 干细胞/细胞学 神经元/细胞学 细胞分化/药物作用
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血红素加氧酶-1在铝过负荷致神经元退行性变中的作用 被引量:2
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作者 袁野 郭建增 +1 位作者 杨俊卿 周岐新 《第四军医大学学报》 北大核心 2008年第7期592-595,共4页
目的:探讨血红素加氧酶-1(HO-1)在铝过负荷致神经元退行性变中变化和意义.方法:雄性NIH小鼠75只,体质量24~26g,随机分为对照组,氯化铝低剂量组(3.75mCd)和高剂量组(7.50mg/d),每组25只.各组分别采取脑室内注射人工脑... 目的:探讨血红素加氧酶-1(HO-1)在铝过负荷致神经元退行性变中变化和意义.方法:雄性NIH小鼠75只,体质量24~26g,随机分为对照组,氯化铝低剂量组(3.75mCd)和高剂量组(7.50mg/d),每组25只.各组分别采取脑室内注射人工脑脊液、1.25g/L氯化铝溶液或2.50g/L氯化铝溶液3μL/只,1次/d,连续5d,建立神经元退行性变模型;以血红素加氧酶(HO)活性和HO-1蛋白表达与丙二醛水平,金属离子含量及海马病理形态学变化关系评价HO-1在铝过负荷致神经元退行性变中的作用.结果:与对照组相比,各氯化铝组均可见海马CA1区细胞层变薄伴明显核固缩.HO-1蛋白:氯化铝低剂量组0.24±0.01,高剂量组0.33±0.01;铁离子含量:低剂量组(31.34±2.85)mg/g,高剂量组(54.01±2.31)mg/g,均较对照组HO-1蛋白0.10±0.01,铁离子含量(22.54±4.48)mg/g显著升高,差异具有统计学意义(P〈0.05).氯化铝高、低剂量组HO活性,丙二醛和铝粒子含量亦均较对照组显著升高,差异具有统计学意义(P〈0.05).结论:HO-1表达上调导致血红素来源铁升高与铝过负荷致神经元退行性变的发生有关. 展开更多
关键词 血红素加氧酶-1 过负荷 神经元退行性变 铝/毒性
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Aluminium tolerance in barley (Hordeum vulgare L.): physiological mechanisms, genetics and screening methods 被引量:7
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作者 WANG Jun-ping RAMAN Harsh +2 位作者 ZHANG Guo-ping MENDHAM Neville ZHOU Mei-xue 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2006年第10期769-787,共19页
Aluminium (Al) toxicity is one of the major limiting factors for barley production on acid soils. It inhibits root cell division and elongation, thus reducing water and nutrient uptake, consequently resulting in poor ... Aluminium (Al) toxicity is one of the major limiting factors for barley production on acid soils. It inhibits root cell division and elongation, thus reducing water and nutrient uptake, consequently resulting in poor plant growth and yield. Plants tolerate Al either through external resistance mechanisms, by which Al is excluded from plant tissues or internal tolerance mechanisms, conferring the ability of plants to tolerate Al ion in the plant symplasm where Al that has permeated the plas- malemma is sequestered or converted into an innocuous form. Barley is considered to be most sensitive to Al toxicity among cereal species. Al tolerance in barley has been assessed by several methods, such as nutrient solution culture, soil bioassay and field screening. Genetic and molecular mapping research has shown that Al tolerance in barley is controlled by a single locus which is located on chromosome 4H. Molecular markers linked with Al tolerance loci have been identified and validated in a range of diverse populations. This paper reviews the (1) screening methods for evaluating Al tolerance, (2) genetics and (3) mechanisms underlying Al tolerance in barley. 展开更多
关键词 BARLEY Al toxicity A1 tolerance
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Chemical Species of Aluminum Ions in Acid Soils *1 被引量:7
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作者 XURENKOU JIGUOLIANG 《Pedosphere》 SCIE CAS CSCD 1998年第2期127-133,共7页
Soil samples collected from several acid soils in Guangdong, Fujian, Zhejiang and Anhui provinces of the southern China were employed to characterize the chemical species of aluminum ions in the soils. The proportio... Soil samples collected from several acid soils in Guangdong, Fujian, Zhejiang and Anhui provinces of the southern China were employed to characterize the chemical species of aluminum ions in the soils. The proportion of monomeric inorganic Al to total Al in soil solution was in the range of 19% to 70%, that of monomeric organic Al (Al OM) to total Al ranged from 7.7% to 69%, and that of the acid soluble Al to total Al was generally smaller and was lower than 20% in most of the acid soils studied. The Al OM concentration in soil solution was positively correlated with the content of dissolved organic carbon (DOC) and also affected by the concentration of Al 3+ . The complexes of aluminum with fluoride (Al F) were the predominant forms of inorganic Al, and the proportion of Al F complexes to total inorganic Al increased with pH. Under strongly acid condition, Al 3+ was also a major form of inorganic Al, and the proportion of Al 3+ to total inorganic Al decreased with increasing pH. The proportions of Al OH and Al SO 4 complexes to total inorganic Al were small and were not larger than 10% in the most acid soils. The concentration of inorganic Al in solution depended largely on pH and the concentration of total F in soil solution. The concentrations of Al OM, Al 3+ ,Al F and Al OH complexes in topsoil were higher than those in subsoil and decreased with the increase in soil depth. The chemical species of aluminum ions were influenced by pH. The concentrations of Al OM, Al 3+ , Al F complexes and Al OH complexes decreased with the increase in pH. 展开更多
关键词 acid soil aluminum ion chemical species
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Effect of Combined Heavy Metal Pollution on Nitrogen Mineralization Potential,Urease and Phosphatase Activities in a Typic Udic Ferrisol 被引量:13
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作者 ZHENG CHUNRONG TU CONG and CHEN HUAIMAN(Laboratory of Material Cycling in Pedosphere, Institute of Soil Science, the Chinese Academy of Sciences,Nanjing 210008 (China))(Received April 20, 1999 revised June 2, 1999) 《Pedosphere》 SCIE CAS CSCD 1999年第3期251-258,共8页
Individual and combined effects of Cu, Pb, Zn and Cd on N mineralization, urease and phosphatase were examined in a Typic Udic Ferrisol in laboratory by employing an uniform design and a single factor design.Soil poll... Individual and combined effects of Cu, Pb, Zn and Cd on N mineralization, urease and phosphatase were examined in a Typic Udic Ferrisol in laboratory by employing an uniform design and a single factor design.Soil pollution caused by heavy metals inhibited N mineralization (No value) and urease and phosphatase activities. The combined pollution of metals alleviated their toxicity to N mineralization to some extent,whereas aggravated the toxicity to urease and phosphatase. Phosphorous application could mitigate the toxic effect of heavy metals on phosphatase activities, while alleviating effect of N application on the toxicity of heavy metals to urease was inconsistent. However, the mitigating effect of the fertilizers was limited in heavily polluted soils. 展开更多
关键词 heavy metal pollution N mineralization PHOSPHATASE UREASE
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Effect of Electrolyte on the Dissolution of Aluminum from Acid Soils and the Distribution of Aluminum Forms inSoil Solution
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作者 XURENKOU JIGUOLIANG 《Pedosphere》 SCIE CAS CSCD 1997年第4期331-338,共8页
KCl, CaCl2, NH4Cl, NaCl, K2SO4 and KF solutions were used for studying the effects of canons and anions on the dissolution of aluminum and the distribution of aluminum forms respectively. Power of exchanging and relea... KCl, CaCl2, NH4Cl, NaCl, K2SO4 and KF solutions were used for studying the effects of canons and anions on the dissolution of aluminum and the distribution of aluminum forms respectively. Power of exchanging and releasing aluminum of four kinds of canons was in the decreasing order Ca2+ >K+ >NH+4 >Na+.The dissolution of aluminum increased with the canon concentration. The adsorption affinity of various soils for aluminum was different. The aluminum in the soil with a stronger adsorption affinity was difficult to be exchanged and released by canons. The Al-F complexes were main species of inorganic aluminum at a low concentration of canons, while Al3+ became major species of inorganic aluminum at a high concentration of canons. The results on the effect of anions indicated that the concentrations of total aluminum, three kinds of inorganic aluminum (Al3+, Al-F and Al-OH complexes) and organic aluminum complexes (Al-OM)when SO2-4 was added into soil suspension were lower than those when Cl- was added. The dissolution of aluminum from soils and the distribution of aluminum forms in solution were edicted by the adsorption of Fon the soil. For soils with strong affinty for F- , the concentrations of the three inorganic aluminum species in soil solution after addition of F- were lower than those after addition of Cl-; but for soils with weak affinity for F- , the concentrations of Al3+ and Al-OM were lower and the concentrations of Al-F complexes and total inorganic aluminum after addition of F- were higher than those after addition of Cl-. The increase of F- concentration in soil solution accelerated the dissolution of aluminum from soils. 展开更多
关键词 acid soil aluminum ionic species dissolution of aluminum ELECTROLYTE
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Statistics of Acute Aluminium Phosphide Poisoning in Fez, Morocco
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作者 Boukatta Brahim El Bouazzaoui Abderrahim +3 位作者 Houari Nawfal Achour Sanae Sbai Hicham Kanjaa Nabil 《Journal of Life Sciences》 2013年第11期1159-1164,共6页
Aluminium phosphide is a rodenticide widely used in agriculture to protect grains and foods. Poisoning with this product represents a real problem in developing countries, as an interested young people and it causes h... Aluminium phosphide is a rodenticide widely used in agriculture to protect grains and foods. Poisoning with this product represents a real problem in developing countries, as an interested young people and it causes high mortality. Aims of this study are to analysis epidemiological aspects, clinics, therapeutic, outcome of this acute poisoning and determine the factors of mortality. It is a retrospective study, concerned 47 patients intoxicated by the Aluminium phosphide between January 2009 and December 2012. It represents 23.4% of all poisonings. The mean age was 24.5 years with a female predominance (63.8%). The poisoning was in a suicidal goal in 95.7% of cases. The mean ingested quantity was 3 grams. The initial clinical picture was dominated by vomiting, abdominal pain, shock and disorders of consciousness in 57.4%, 48.9%, 31.9% and 21.3%, respectively. Electrocardiogram abnormalities were observed in 38.3% of cases. Increase of Troponin was observed in 27.7% of cases. 36.2% of patients were intubated and ventilated and 40.4% have been placed under vasoactive drugs. The mortality rate was 38.3%. The prognostic factors were: delay of management, potassium disorders, elevated of Troponin, hypotension, tachycardia, need for mechanical ventilation, vasoactive drugs and shock. To improve the prognosis, the management should be rapid and the governement should focus more on prevention, so the aluminium phosphide will not be avaible to general population. 展开更多
关键词 Acute poisoning aluminium phosphide MORTALITY PREVENTION
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Potential of Industrial Byproducts in Ameliorating Acidity and Aluminum Toxicity of Soils Under Tea Plantation 被引量:42
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作者 LI Jiu-Yu WANG Ning +1 位作者 XU Ren-Kou D. TIWARI 《Pedosphere》 SCIE CAS CSCD 2010年第5期645-654,共10页
It is imperative to choose some low cost, available and effective ameliorants to correct soil acidity in southern China for sustainable agriculture. The present investigation dealt with the possible role of industrial... It is imperative to choose some low cost, available and effective ameliorants to correct soil acidity in southern China for sustainable agriculture. The present investigation dealt with the possible role of industrial byproducts, i.e., coal fly ash (CFA), alkaline slag (AS), red mud (RM) and phosphogypsum (PG) in correcting acidity and aluminum (Al) toxicity of soils under tea plantation using an indoor incubation experiment. Results indicated that CFA, AS and RM increased soil pH, while PG decreased the pHs of an Ultisol and an Alfisol. The increment of soil pH followed the order of RM 〉 AS 〉 CFA. All the industrial byproducts invariably decreased exchangeable Al and hence increased exchangeable Ca, Mg, K and Na and effective cation exchange capacity. RM, AS and lime decreased total soluble Al, exchangeable Al and organically bound Al. Formation and retention of hydroxyl-Al polymers were the principal mechanism through which Al phytotoxicity was alleviated by application of these amendments. In addition, the heavy metal contents in the four industrial byproducts constituted a limited environmental hazard in a short time at the rates normally used in agriculture. Therefore, the short-term use of the byproducts, especially AS and RM, as amendments for soil acidity and AI toxicity in acid soils may be a potential alternative to the traditional use of mined gypsum and lime. 展开更多
关键词 acid soil alkaline slag AMELIORATION coal fly ash red mud
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Mechanisms and regulation of aluminum-induced secretion of organic acid anions from plant roots 被引量:11
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作者 Jian-li YANG Wei FAN Shao-jian ZHENG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2019年第6期513-527,共15页
Aluminum(Al)is the most abundant metal element in the earth’s crust.On acid soils,at pH 5.5 or lower,part of insoluble Al-containing minerals become solubilized into soil solution,with resultant highly toxic effects ... Aluminum(Al)is the most abundant metal element in the earth’s crust.On acid soils,at pH 5.5 or lower,part of insoluble Al-containing minerals become solubilized into soil solution,with resultant highly toxic effects on plant growth and development.Nevertheless,some plants have developed Al-tolerance mechanisms that enable them to counteract this Al toxicity.One such well-documented mechanism is the Al-induced secretion of organic acid anions,including citrate,malate,and oxalate,from plant roots.Once secreted,these anions chelate external Al ions,thus protecting the secreting plant from Al toxicity.Genes encoding the citrate and malate transporters responsible for secretion have been identified and characterized,and accumulating evidence indicates that regulation of the expression of these transporter genes is critical for plant Al tolerance.In this review,we outline the recent history of research into plant Al-tolerance mechanisms,with special emphasis on the physiology of Al-induced secretion of organic acid anions from plant roots.In particular,we summarize the identification of genes encoding organic acid transporters and review current understanding of genes regulating organic acid secretion.We also discuss the possible signaling pathways regulating the expression of organic acid transporter genes. 展开更多
关键词 Acid soil Aluminum(Al)toxicity Expression regulation Organic acid anion TRANSPORTER
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