We have synthesized a novel formulation of nanoparticulate（NP） alum adjuvant specifically in the cationic water-in-oil microemulsions of water/benzalkonium bromide（BB）, octyl alcohol/cyclohexane at 30 ℃, which wa...We have synthesized a novel formulation of nanoparticulate（NP） alum adjuvant specifically in the cationic water-in-oil microemulsions of water/benzalkonium bromide（BB）, octyl alcohol/cyclohexane at 30 ℃, which was characterized to be crystalline Al（OH）3 with average particle diameter about 70 nm by the analysis of X-ray diffraction and to have a spherical shape structure by TEM photography analysis. Then adsorption degree was assayed. The OD492 of the NP is about 10 times higher than the bulk（BK） alum adjuvant. Compared to bulk alum adjuvant, we studied the immune effect of the NP alum adjuvant with HBsAg vaccine, which was injected intraperitoneally into Guinea pig, and serum antibody titers of the first and second week after immunization were higher in NP group than BK group by ELISA（P<0\^01, P<0\^05）; Groin immuno-injection intraperitoneally into Balb/c mice, was performed once or there times, prolife responses of spleen cells were determined by （）3H thymidine incorporation; non-specific phagocytosis of macrophage of abdominal cavity was measured by lack of color Malachite Green and IL-2 was assayed by activation mouse spleen cells. It is indicated that all cellular immunity bioassay results were higher in NP group than in BK group, except non-specific phagocytosis of macrophage of abdominal cavity by there times immunity. However, there was no significant differerces. Thus, nanoparticulate alum adjuvant can be employed as a more effective adjuvant for HBsAg vaccine than bulk alum adjuvant in the earlier period of post-immunity.展开更多
铝盐用于疫苗佐剂已有80多年,是目前FDA(Food and drug administration,FDA)批准的唯一疫苗佐剂。多种研究证明铝佐剂能够显著地降低抗原用量,提高免疫应答,并且工艺简单,成本低廉,但是其弊端也渐渐暴露。目前,疫苗的发展越来越受到人...铝盐用于疫苗佐剂已有80多年,是目前FDA(Food and drug administration,FDA)批准的唯一疫苗佐剂。多种研究证明铝佐剂能够显著地降低抗原用量,提高免疫应答,并且工艺简单,成本低廉,但是其弊端也渐渐暴露。目前,疫苗的发展越来越受到人们重视,为了更有效地使铝佐剂在疫苗中发挥辅佐作用,本文综述了铝佐剂的特性、机理及其影响因素。展开更多
文摘We have synthesized a novel formulation of nanoparticulate（NP） alum adjuvant specifically in the cationic water-in-oil microemulsions of water/benzalkonium bromide（BB）, octyl alcohol/cyclohexane at 30 ℃, which was characterized to be crystalline Al（OH）3 with average particle diameter about 70 nm by the analysis of X-ray diffraction and to have a spherical shape structure by TEM photography analysis. Then adsorption degree was assayed. The OD492 of the NP is about 10 times higher than the bulk（BK） alum adjuvant. Compared to bulk alum adjuvant, we studied the immune effect of the NP alum adjuvant with HBsAg vaccine, which was injected intraperitoneally into Guinea pig, and serum antibody titers of the first and second week after immunization were higher in NP group than BK group by ELISA（P<0\^01, P<0\^05）; Groin immuno-injection intraperitoneally into Balb/c mice, was performed once or there times, prolife responses of spleen cells were determined by （）3H thymidine incorporation; non-specific phagocytosis of macrophage of abdominal cavity was measured by lack of color Malachite Green and IL-2 was assayed by activation mouse spleen cells. It is indicated that all cellular immunity bioassay results were higher in NP group than in BK group, except non-specific phagocytosis of macrophage of abdominal cavity by there times immunity. However, there was no significant differerces. Thus, nanoparticulate alum adjuvant can be employed as a more effective adjuvant for HBsAg vaccine than bulk alum adjuvant in the earlier period of post-immunity.
文摘铝盐用于疫苗佐剂已有80多年,是目前FDA(Food and drug administration,FDA)批准的唯一疫苗佐剂。多种研究证明铝佐剂能够显著地降低抗原用量,提高免疫应答,并且工艺简单,成本低廉,但是其弊端也渐渐暴露。目前,疫苗的发展越来越受到人们重视,为了更有效地使铝佐剂在疫苗中发挥辅佐作用,本文综述了铝佐剂的特性、机理及其影响因素。
