目的观察单纯型糖尿病性视网膜病变(DR)的光谱视网膜电图(ERG)改变特点。方法采用自然瞳孔和相同刺激光强度,在暗适应和明适应两种条件下,DR 组和对照组分别依次给予蓝光、绿光和红光刺激。皮肤电极记录,t 检验分析。结果 DR 组红光 ERG...目的观察单纯型糖尿病性视网膜病变(DR)的光谱视网膜电图(ERG)改变特点。方法采用自然瞳孔和相同刺激光强度,在暗适应和明适应两种条件下,DR 组和对照组分别依次给予蓝光、绿光和红光刺激。皮肤电极记录,t 检验分析。结果 DR 组红光 ERG 暗适应 a 波振幅P=0.05,b 波振幅暗适应和明适应 P<0.01;绿光 ERG 暗适应 a 波振幅 P<0.01,b 波振幅暗适应和明适应 P<0.01,明适应 b 波峰潜时 P<0.01,暗适应 b 波峰潜时 P<0.05;蓝光 ERG 暗适应 a 波振幅 P<0.05,b 波振幅 P<0.05,结论光谱 ERG 可以作为不同时期 DR 的不同波长锥细胞损害情况的评价指标。展开更多
[Objective] This study was conducted to investigate the effects of Echi-nacea purpurea polysaccharides （EPS） on proliferation of rat intestinal epithelial cel IEC-6. [Method] The proliferation rate of IEC-6 cel s cu...[Objective] This study was conducted to investigate the effects of Echi-nacea purpurea polysaccharides （EPS） on proliferation of rat intestinal epithelial cel IEC-6. [Method] The proliferation rate of IEC-6 cel s cultured in EPS at different concentrations and for different time was measured by MTT assay and analyzed by statistic methods. [Result] The proliferation rate of IEC-6 cel s cultured in EPS at al the concentrations and for different time was improved by different extents in com-parison with the control. In detail, 50 and 200 μg/ml EPS greatly improved the IEC-6 cel proliferation after 24 h of culture; then, the cel proliferation rate in the two treatments increased from 24 to 48 h, and declined from 48 to 72 h. The cel pro-liferation was also significantly improved by culturing in 100 μg/ml EPS for 72 h and in 500 μg/ml EPS for 48 h. After 48 h of culture, the proliferation rate of IEC-6 cel increased in a EPS dose-dependent manner. [Conclusion] EPS can promote IEC-6 cel proliferation, and thus improve the intestinal mucosal absorption and immune function of rat.展开更多
文摘1临床资料患者,男性,53岁。因双眼夜间视力差15~16年,畏强光8~9个月,于2014年6月11日来南昌大学第一附属医院眼科就诊。有糖尿病史10年,一直给予胰岛素控制,自诉血糖控制平稳。眼科检查：右眼视力0.12,左眼视力0.12,双眼矫正视力无提高。双眼位正,结膜无充血,角膜清亮,前房中深,房水清,瞳孔圆,大小约3.5 mm,
文摘目的观察单纯型糖尿病性视网膜病变(DR)的光谱视网膜电图(ERG)改变特点。方法采用自然瞳孔和相同刺激光强度,在暗适应和明适应两种条件下,DR 组和对照组分别依次给予蓝光、绿光和红光刺激。皮肤电极记录,t 检验分析。结果 DR 组红光 ERG 暗适应 a 波振幅P=0.05,b 波振幅暗适应和明适应 P<0.01;绿光 ERG 暗适应 a 波振幅 P<0.01,b 波振幅暗适应和明适应 P<0.01,明适应 b 波峰潜时 P<0.01,暗适应 b 波峰潜时 P<0.05;蓝光 ERG 暗适应 a 波振幅 P<0.05,b 波振幅 P<0.05,结论光谱 ERG 可以作为不同时期 DR 的不同波长锥细胞损害情况的评价指标。
基金Supported by National Natural Science Foundation of China(31472230)Natural Science Foundation of Hebei Province(C2014407068)+1 种基金Fund of Department of Science and Technology of Hebei Province(14966610D,12220408D)Fund from Hebei Provincial Department of Education for Hundreds of Outstanding Innovative Talents(II)(ZH2011244,Q2012037)~~
文摘[Objective] This study was conducted to investigate the effects of Echi-nacea purpurea polysaccharides （EPS） on proliferation of rat intestinal epithelial cel IEC-6. [Method] The proliferation rate of IEC-6 cel s cultured in EPS at different concentrations and for different time was measured by MTT assay and analyzed by statistic methods. [Result] The proliferation rate of IEC-6 cel s cultured in EPS at al the concentrations and for different time was improved by different extents in com-parison with the control. In detail, 50 and 200 μg/ml EPS greatly improved the IEC-6 cel proliferation after 24 h of culture; then, the cel proliferation rate in the two treatments increased from 24 to 48 h, and declined from 48 to 72 h. The cel pro-liferation was also significantly improved by culturing in 100 μg/ml EPS for 72 h and in 500 μg/ml EPS for 48 h. After 48 h of culture, the proliferation rate of IEC-6 cel increased in a EPS dose-dependent manner. [Conclusion] EPS can promote IEC-6 cel proliferation, and thus improve the intestinal mucosal absorption and immune function of rat.
