A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidylox...A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C 2H 5) 3, 2 mmol/L HAc and 0 2 mmol/L Cu(Ac) 2 as the mobile phase with a flow rate 1 0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL amino acids on the CBSP was found to be between 1 11 and 1 51. The elution order of D isomer before L isomer on the CBSP was observed for all the DL amino acids resolved except DL Val. For DL Pro, DL Val and DL Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L Pro or L hydroxyl proline with a five\|membered ring structure. [WT5HZ]展开更多
The macrocyclic dioxopolyamine compounds, 1,4,7,10-tetraazacyclotridecane-11,13-diones were used as stationary phases with the aid of 3-(2-cyclooxypropoxyl)propyl-trimethoxy silane as a bridge in open-tubular capillar...The macrocyclic dioxopolyamine compounds, 1,4,7,10-tetraazacyclotridecane-11,13-diones were used as stationary phases with the aid of 3-(2-cyclooxypropoxyl)propyl-trimethoxy silane as a bridge in open-tubular capillary electrochromatography(OTCEC). The capillaries were etched by aqueous sodium before being coated. These modified capillaries had shown improved separations of neurotransmitters, isomeric dihydrobenzenes, isomeric nitrophenols and isomeric aminophenols in comparison with untreated capillaries.展开更多
A calix[8]arene-bonded gel silica stationary phase(DCABS) was prepared by using coupling reagent(KH560). Its structure was characterized by FTIR, elemental analysis and thermal analysis. The chromatographic performanc...A calix[8]arene-bonded gel silica stationary phase(DCABS) was prepared by using coupling reagent(KH560). Its structure was characterized by FTIR, elemental analysis and thermal analysis. The chromatographic performance of DCABS was studied by using different solutes as probes and stationary phases as reference. The results show that the new material has an excellent reversed-phase chromatographic property. The new bonded phase can provide various sites for analytes, such as the hydrophobic interaction, hydrogen-bonding, π-π, electrostatic interactions. Meanwhile, it was observed that the chromatographic performance of DCABS is partially different from CABS because of elimination of the butyl groups.展开更多
文摘A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C 2H 5) 3, 2 mmol/L HAc and 0 2 mmol/L Cu(Ac) 2 as the mobile phase with a flow rate 1 0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL amino acids on the CBSP was found to be between 1 11 and 1 51. The elution order of D isomer before L isomer on the CBSP was observed for all the DL amino acids resolved except DL Val. For DL Pro, DL Val and DL Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L Pro or L hydroxyl proline with a five\|membered ring structure. [WT5HZ]
文摘The macrocyclic dioxopolyamine compounds, 1,4,7,10-tetraazacyclotridecane-11,13-diones were used as stationary phases with the aid of 3-(2-cyclooxypropoxyl)propyl-trimethoxy silane as a bridge in open-tubular capillary electrochromatography(OTCEC). The capillaries were etched by aqueous sodium before being coated. These modified capillaries had shown improved separations of neurotransmitters, isomeric dihydrobenzenes, isomeric nitrophenols and isomeric aminophenols in comparison with untreated capillaries.
文摘A calix[8]arene-bonded gel silica stationary phase(DCABS) was prepared by using coupling reagent(KH560). Its structure was characterized by FTIR, elemental analysis and thermal analysis. The chromatographic performance of DCABS was studied by using different solutes as probes and stationary phases as reference. The results show that the new material has an excellent reversed-phase chromatographic property. The new bonded phase can provide various sites for analytes, such as the hydrophobic interaction, hydrogen-bonding, π-π, electrostatic interactions. Meanwhile, it was observed that the chromatographic performance of DCABS is partially different from CABS because of elimination of the butyl groups.
