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Omeprazole decreases magnesium transport across Caco-2 monolayers 被引量:4
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作者 Narongrit Thongon Nateetip Krishnamra 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第12期1574-1583,共10页
AIM:To elucidate the effect and underlying mechanisms of omeprazole action on Mg 2+ transport across the intestinal epithelium.METHODS:Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14... AIM:To elucidate the effect and underlying mechanisms of omeprazole action on Mg 2+ transport across the intestinal epithelium.METHODS:Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14 or 21 d before being inserted into a modified Ussing chamber apparatus to investigate the bi-directional Mg 2+ transport and electrical parameters.Paracellular permeability of the monolayer was also observed by the dilution potential technique and a cation permeability study.An Arrhenius plot was performed to elucidate the activation energy of passive Mg 2+ transport across the Caco-2 monolayers.RESULTS:Both apical to basolateral and basolateral to apical passive Mg 2+ fluxes of omeprazole-treated epithelium were decreased in a dose-and time-dependent manner.Omeprazole also decreased the paracellular cation selectivity and changed the paracellular selective permeability profile of Caco-2 epithelium to Li +,Na +,K +,Rb +,and Cs + from seriesⅦto seriesⅥof the Eisenman sequence.The Arrhenius plot revealed the higher activation energy for passive Mg 2+ transport in omeprazoletreated epithelium than that of control epithelium,indicating that omeprazole affected the paracellular channel of Caco-2 epithelium in such a way that Mg 2+ movement was impeded.CONCLUSION:Omeprazole decreased paracellular cation permeability and increased the activation energy for passive Mg 2+ transport of Caco-2 monolayers that led to the suppression of passive Mg 2+ absorption. 展开更多
关键词 MAGNESIUM PARACELLULAR Proton pump inhibitor Transepithelial Tight junction
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脑梗死患者血浆内皮素水平及钠钾泵钙泵活性改变 被引量:5
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作者 何国厚 王万铭 +1 位作者 刘久波 严世荣 《卒中与神经疾病》 2000年第2期120-121,共2页
目的 :探讨脑梗死患者血浆内皮素水平变化及红细胞膜钠钾泵、钙泵活性改变及临床意义。方法 :应用非平衡法测定了 4 1例脑梗死患者及 2 6例对照组的血浆内皮素水平和红细胞膜 Na+-K+ATPase、Ca2 +-Mg2 +ATPase活性测定。结果 :脑梗死组... 目的 :探讨脑梗死患者血浆内皮素水平变化及红细胞膜钠钾泵、钙泵活性改变及临床意义。方法 :应用非平衡法测定了 4 1例脑梗死患者及 2 6例对照组的血浆内皮素水平和红细胞膜 Na+-K+ATPase、Ca2 +-Mg2 +ATPase活性测定。结果 :脑梗死组血浆内皮素水平明显高于对照组 ,红细胞膜 Na+-K+ATPase和 Ca2 +-Mg2 +ATPase活性明显低于对照组 ,而且血浆内皮素的水平增高与 Na+-K+ATPase和 Ca2 +-Mg2 +ATPase活性呈负相关 ,与 SBP、DBP呈正相关。结论 :内皮素通过收缩血管 ,升高血压 ,影响红细胞膜离子泵活性导致脑血流下降 。 展开更多
关键词 脑梗死 内皮素 三磷酸腺苷酶类 镁泵
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长期口服卡托普利对小鼠脑组织自由基及相关酶的影响 被引量:2
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作者 谢湘林 马春颖 +3 位作者 周鸣 叶秋芳 赵莉 刘宏雁 《中国药房》 CAS CSCD 北大核心 2011年第1期27-28,共2页
目的:探讨长期口服卡托普利对小鼠脑组织自由基及相关酶的影响,为其临床应用提供实验依据。方法:取30只小鼠,随机均分为正常对照组(水)和卡托普利组(卡托普利,11.08~14.92mg·kg-1·d-1),按饮水给药方式连续给药14周,测定脑组... 目的:探讨长期口服卡托普利对小鼠脑组织自由基及相关酶的影响,为其临床应用提供实验依据。方法:取30只小鼠,随机均分为正常对照组(水)和卡托普利组(卡托普利,11.08~14.92mg·kg-1·d-1),按饮水给药方式连续给药14周,测定脑组织中自由基与相关酶(丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、钠-钾泵三磷酸腺苷酶(Na+-K+-ATP酶)、钙-镁泵三磷酸腺苷酶(Ca2+-Mg2+-ATP酶))水平。结果:与正常对照组比较,卡托普利组小鼠脑组织中MDA水平降低(P<0.05);SOD、GSH-Px水平均无明显变化;Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶活性有降低的趋势,但无统计学意义。结论:长期口服卡托普利可减少脑组织自由基含量,增强机体抗氧化能力,但脑组织神经细胞膜功能有受损的可能。 展开更多
关键词 卡托普利 自由基 小鼠 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 钠-钾三磷酸腺苷酶 钙-镁泵三磷酸腺苷酶
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