The highly-dispersed iron element decorated Ni foam was prepared by simple immersion in a ferric nitrate solution at room temperature without using acid etching, and characterized by X-ray powder diffraction(XRD), sca...The highly-dispersed iron element decorated Ni foam was prepared by simple immersion in a ferric nitrate solution at room temperature without using acid etching, and characterized by X-ray powder diffraction(XRD), scanning electron microscopy(SEM), EDAX spectrum(EDAX mapping) and Raman spectroscopy. The EDAX spectrum illustrated that iron element was highly-dispersed over the entire surface of nickel foam, and the Raman spectroscopy revealed that both Ni-O and Fe-O bonds were formed on the surface of the as-prepared electrode. Moreover, the iron element decorated Ni foam electrode can be used as non-enzymatic glucose sensor and it exhibits not only an ultra-wide linear concentration range of 1-18 mmol/L with an outstanding sensitivity of 1.0388 m A·mmol/(L·cm2), but also an excellent ability of stability and selectivity. Therefore, this work presents a simple yet effective approach to successfully modify Ni foam as non-enzymatic glucose sensor.展开更多
The complete [ NiFe]-hydrogenase genes were cloned from cyanobacterium, Arthrospira platensis FACHB341, using PCR and in-vitro cloning. The total length is 2078bp, including hoxY 549bp, hoxH 1431bp and the spacer in b...The complete [ NiFe]-hydrogenase genes were cloned from cyanobacterium, Arthrospira platensis FACHB341, using PCR and in-vitro cloning. The total length is 2078bp, including hoxY 549bp, hoxH 1431bp and the spacer in between 98bp. There is a stem-loop structure, downstream of the hoxY gene, serving as a transcription terminator. The deduced amino acid sequences of HoxY aud HoxH consist of acidic amino acid of 15.8% and 12.0%; alkalitropic amino acid of 11.9% and 15.3%; hydrophobic amino acid of 46.3% and 41.0%, respectively. The similarities of hoxY and hoxH genes in Arthrospira platensis FACHB341 to their homologues in other cyanobacteria were compared respectively. The secondary structures and 3D models of small and large subunits of [ NiFe]-hydrogenase were predicted by using 3D-PSSM.展开更多
基金Project(2019zzts684)supported by the Fundamental Research Funds for the Central Universities,China。
文摘The highly-dispersed iron element decorated Ni foam was prepared by simple immersion in a ferric nitrate solution at room temperature without using acid etching, and characterized by X-ray powder diffraction(XRD), scanning electron microscopy(SEM), EDAX spectrum(EDAX mapping) and Raman spectroscopy. The EDAX spectrum illustrated that iron element was highly-dispersed over the entire surface of nickel foam, and the Raman spectroscopy revealed that both Ni-O and Fe-O bonds were formed on the surface of the as-prepared electrode. Moreover, the iron element decorated Ni foam electrode can be used as non-enzymatic glucose sensor and it exhibits not only an ultra-wide linear concentration range of 1-18 mmol/L with an outstanding sensitivity of 1.0388 m A·mmol/(L·cm2), but also an excellent ability of stability and selectivity. Therefore, this work presents a simple yet effective approach to successfully modify Ni foam as non-enzymatic glucose sensor.
文摘The complete [ NiFe]-hydrogenase genes were cloned from cyanobacterium, Arthrospira platensis FACHB341, using PCR and in-vitro cloning. The total length is 2078bp, including hoxY 549bp, hoxH 1431bp and the spacer in between 98bp. There is a stem-loop structure, downstream of the hoxY gene, serving as a transcription terminator. The deduced amino acid sequences of HoxY aud HoxH consist of acidic amino acid of 15.8% and 12.0%; alkalitropic amino acid of 11.9% and 15.3%; hydrophobic amino acid of 46.3% and 41.0%, respectively. The similarities of hoxY and hoxH genes in Arthrospira platensis FACHB341 to their homologues in other cyanobacteria were compared respectively. The secondary structures and 3D models of small and large subunits of [ NiFe]-hydrogenase were predicted by using 3D-PSSM.