海洋真菌Fusarium sp.次级代谢产物的研究

采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、开放ODS柱色谱以及HPLC等方法从海洋真菌Fusariumsp.的菌丝体中分离得到5个化合物,通过波谱数据及理化性质分别鉴定为3β,15β-二羟基-(22E,24R)-麦角甾-5,8(14),22-三烯-7-酮(1)、3β,5α,9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(2)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(3)、5α,8α-过氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇(4)和丁二酸(5)。其中化合物1和2首次从该属真菌中分离得到。

海洋来源真菌Fusarium sp.次级代谢产物的研究(Ⅱ)

采用多种色谱技术对海洋来源真菌Fusariumsp.的发酵物进行化学成分分离,通过波谱及理化性质分析确定了化合物的结构。从菌丝体的丙酮提取物中共分离得到10个化合物,分别为1,3,5-三羟基-7-甲基蒽醌(1)、6-羟基芦荟大黄素(2)、3-O-β-D-吡喃葡萄糖基-豆甾-5,24(28)Z-二烯(3)、尿嘧啶核苷(4)、次黄嘌呤核苷(5)、吡咯并哌嗪-2,5-二酮(6)、3-异丙基吡咯并哌嗪-2,5-二酮(7)、尿嘧啶(8)、10S-羟基十八碳酸(9)、甘露醇(10)。其中化合物1、3和9均为首次从该属真菌中分离得到。

Analysis of endophytic fungi in roots of Santalum album Linn.and its host plant Kuhnia rosmarinifolia Vent

Santalum album Linn. is an evergreen and hemi-parasitic tree, the heartwood-sandalwood of which was used during a long history in traditional Chinese medicine. Kuhnia rosmarinifolia Vent. is a good host for 1- or 2-year-old growing S. album. The interaction between S. album and K. rosmarinifolia is still little known. Many studies have been carried out on a number of plants for identification and diversity of endophytes. In this study, in total 25 taxa of endophytic fungi were isolated from the roots of S. album and the roots of K. rosmarinifolia. The most frequently isolated genera were Penicillium sp. 1 and Fusarium sp. 1 in the roots of S. album and K. rosmadnifolia, respectively. S. album is a root parasite of K. rosmarinifolia. The interesting result is that they apparently do not share the same endophytic fungi isolates. This study for the first time explored the content of endophytic fungi from S. album and K. rosmarinifolia, which provides important information for further studies.