In laboratory conditions,effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively.Data series like larval ...In laboratory conditions,effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively.Data series like larval length and density,metamorphic time,and survival rate of the larvae were recorded.Statistics showed that for A.japonicus,survival rate(from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature(P<0.05).At different temperatures SGR was statistically significant as well(P<0.05) from day 1,and maximal SGR was found on day 9 at 24℃(159.26±3.28).This study clearly indicated that at low temperature(<24℃),metamorphic rate was remarkably higher than at higher temperature(>26℃).Hatching rate was significantly different between 0.2-5 ind./ml groups and 20-50 ind./ml groups.Rearing larvae at the higher density had the smaller maximal-length,whereas needed longer time to complete metamorphosis.This study suggested that 21℃ and 0.4 ind./ml can be used as the most suitable rearing temperature and stocking density for large-scale artificial breeding of A.japonicus's larvae.展开更多
During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic ...During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic （18S rDNA and 16S rDNA sequences） analyses revealed a close affinity with Eutreptiella gymnastica, a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20C, 160 gmol photons/（mE.s） of white light, and a salinity of 10-31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 gmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment.展开更多
In the study cyanobacterial strains were isolated from different sites of Hizoop rivers, Koya-Iraq and identified according to their morphological characters by using microscope, two genera which were in filamentous f...In the study cyanobacterial strains were isolated from different sites of Hizoop rivers, Koya-Iraq and identified according to their morphological characters by using microscope, two genera which were in filamentous form identified as Chroococcus sp. and Lyngbya sp.. After identification of genera their optimum growth condition studied by using the effect of temperature and pH to their dry weight. In the result, the optimum temperature and pH for both filamentous cyanobacteria were 25 ℃ and pH 7.5. Both cyanobacterial strains were extracted with ethanol, methanol and diethyl ether at various concentrations （0.2 g/mL, 0.1 g/mL, 0.005 g/mL） which exhibited the antibacterial activities against Staphylococcus aureus, E. coli and B. subtilus. Inhibition activities of the two cyanobacterial extracts were more effective at high concentration against the tested pathogens at the low concentration, especially those ofLyngbya sp. The higher inhibition zone showed with extract by ethanol.展开更多
In weak acidic medium the alkaline cationic Safranine T interacts with acidic biological rnacromolecule heparin intensively causing the change of the molecular conformation, the maximum absorption wavelength and the a...In weak acidic medium the alkaline cationic Safranine T interacts with acidic biological rnacromolecule heparin intensively causing the change of the molecular conformation, the maximum absorption wavelength and the absorption value. The optimum experimental condition was given and a new method to determine the heparin was established. The linear range was 0.05-2.0 mg/L and the correlation coefficient was 0.9974. The method has been applied to analyze the samples and the results are satisfactory. Furthermore, the interaction mechanism was indicated.展开更多
Waterlogging is a major abiotic stress limiting barley (Hordeum vulgare L.) yield and its stability in areas with excessive rainfall.Identification of genomic regions influencing the response of yield and its componen...Waterlogging is a major abiotic stress limiting barley (Hordeum vulgare L.) yield and its stability in areas with excessive rainfall.Identification of genomic regions influencing the response of yield and its components to waterlogging stress will enhance our understanding of the genetics of waterlogging tolerance and the development of more tolerant barley cultivars.Quantitative trait loci (QTLs) for grain yield and its components were identified using 156 doubled haploid (DH) lines derived from a cross between the cultivars Yerong (waterlogging-tolerant) and Franklin (waterlogging-sensitive) grown under different conditions (waterlogged and well drained).A total of 31 QTLs were identified for the measured characters from two experiments with two growth environments.The phenotypic variation explained by individual QTLs ranged from 4.74% to 55.34%.Several major QTLs determining kernel weight (KW),grains per spike (GS),spikes per plant (SP),spike length (SL) and grain yield (GY) were detected on the same region of chromosome 2H,indicating close linkage or pleiotropy of the gene(s) controlling these traits.Some different QTLs were identified under waterlogging conditions,and thus different markers may have to be used in selecting cultivars suitable for high rainfall areas.展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2006AA10A411)Shandong Agriculture Seed Stock Projects
文摘In laboratory conditions,effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively.Data series like larval length and density,metamorphic time,and survival rate of the larvae were recorded.Statistics showed that for A.japonicus,survival rate(from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature(P<0.05).At different temperatures SGR was statistically significant as well(P<0.05) from day 1,and maximal SGR was found on day 9 at 24℃(159.26±3.28).This study clearly indicated that at low temperature(<24℃),metamorphic rate was remarkably higher than at higher temperature(>26℃).Hatching rate was significantly different between 0.2-5 ind./ml groups and 20-50 ind./ml groups.Rearing larvae at the higher density had the smaller maximal-length,whereas needed longer time to complete metamorphosis.This study suggested that 21℃ and 0.4 ind./ml can be used as the most suitable rearing temperature and stocking density for large-scale artificial breeding of A.japonicus's larvae.
基金Supported by the National Special Research Fund for Non-Profit Sector(Agriculture) (No. 200903030)the National Key Basic Program of Science & Technology-Platforms of Aquaculture Stock Resourcesa Project from Chinese Academy of Sciences
文摘During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic （18S rDNA and 16S rDNA sequences） analyses revealed a close affinity with Eutreptiella gymnastica, a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20C, 160 gmol photons/（mE.s） of white light, and a salinity of 10-31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 gmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment.
文摘In the study cyanobacterial strains were isolated from different sites of Hizoop rivers, Koya-Iraq and identified according to their morphological characters by using microscope, two genera which were in filamentous form identified as Chroococcus sp. and Lyngbya sp.. After identification of genera their optimum growth condition studied by using the effect of temperature and pH to their dry weight. In the result, the optimum temperature and pH for both filamentous cyanobacteria were 25 ℃ and pH 7.5. Both cyanobacterial strains were extracted with ethanol, methanol and diethyl ether at various concentrations （0.2 g/mL, 0.1 g/mL, 0.005 g/mL） which exhibited the antibacterial activities against Staphylococcus aureus, E. coli and B. subtilus. Inhibition activities of the two cyanobacterial extracts were more effective at high concentration against the tested pathogens at the low concentration, especially those ofLyngbya sp. The higher inhibition zone showed with extract by ethanol.
文摘In weak acidic medium the alkaline cationic Safranine T interacts with acidic biological rnacromolecule heparin intensively causing the change of the molecular conformation, the maximum absorption wavelength and the absorption value. The optimum experimental condition was given and a new method to determine the heparin was established. The linear range was 0.05-2.0 mg/L and the correlation coefficient was 0.9974. The method has been applied to analyze the samples and the results are satisfactory. Furthermore, the interaction mechanism was indicated.
基金Project supported by the Hi-Tech Research and Development Pro-gram (863) of China (No.2006AA10Z1C3)the Ministry of Education and the State Administration of Foreign Experts Affairs (111 Project) of China (No.B06014)the Zhejiang Provincial Department of Education Project (No.20070214),China
文摘Waterlogging is a major abiotic stress limiting barley (Hordeum vulgare L.) yield and its stability in areas with excessive rainfall.Identification of genomic regions influencing the response of yield and its components to waterlogging stress will enhance our understanding of the genetics of waterlogging tolerance and the development of more tolerant barley cultivars.Quantitative trait loci (QTLs) for grain yield and its components were identified using 156 doubled haploid (DH) lines derived from a cross between the cultivars Yerong (waterlogging-tolerant) and Franklin (waterlogging-sensitive) grown under different conditions (waterlogged and well drained).A total of 31 QTLs were identified for the measured characters from two experiments with two growth environments.The phenotypic variation explained by individual QTLs ranged from 4.74% to 55.34%.Several major QTLs determining kernel weight (KW),grains per spike (GS),spikes per plant (SP),spike length (SL) and grain yield (GY) were detected on the same region of chromosome 2H,indicating close linkage or pleiotropy of the gene(s) controlling these traits.Some different QTLs were identified under waterlogging conditions,and thus different markers may have to be used in selecting cultivars suitable for high rainfall areas.
