RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication, DNA repair, and RNA processing. However, the function of DEAD-box proteins in aquaculture specie...RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication, DNA repair, and RNA processing. However, the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level. We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins, Fc-vasa and Fc-PL10a, from the testes of Chinese shrimp, Fenneropenaeus chinensis. The two predicted amino acid sequences contain all the conserved motifs characterized by the DEAD-box family and several RGG repeats in the N-terminal regions. Homology and phylogenetic analyses indicate that they belong to the vasa and PLIO subfamilies. The three-dimensional structures of the two proteins were predicted with a homology modeling approach. Both core proteins consist of two tandem RecA-like domains similar to those of the DEAD-box RNA helicase. Using reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR we found that Fc-vasa was expressed specifically in the adult gonads. Transcription decreased in the ovary but increased in the testis during gonadal development. Fc-PL10a expression was widely distributed in the tissues we examined. Using in situ hybridization, we demonstrated that the Fc-vasa transcript is localized to the cytoplasm of the spermatogonia and oocytes. Thus, our results suggest that Fc-wasa plays an important role in germ-line development, and has utility as a germ cell lineage marker which will help to generate new insight into the origin and differentiation of germ cells as well as the regulation of reproduction in F. chinensis.展开更多
Quantifying the functional relationships relating river discharge and weathering products places key constraints on the negative feedback between the silicate weathering and climate. In this study we analyze the conce...Quantifying the functional relationships relating river discharge and weathering products places key constraints on the negative feedback between the silicate weathering and climate. In this study we analyze the concentration–discharge relationships of weathering products from global rivers using previously compiled time-series datasets for concentrations and discharge from global rivers. To analyze the nature of the covariation between specific discharge and concentrations, we use both a power law equation and a recently developed solute production equation. The solute production equation allows us to quantify weathering efficiency, or the resistance to dilution at high runoff, via the Damkohler coefficient. These results are also compared to those derived using average concentration–discharge pairs.Both the power law exponent and the Damkohler coefficient increase and asymptote as catchments exhibit increasingly chemostatic behavior, resulting in an inverse relationship between the two parameters. We also show that using thedistribution of average concentration–discharge pairs from global rivers, rather than fitting concentration–discharge relationships for each individual river, underestimates global median weathering efficiency by up to a factor of ~10. This study demonstrates the utility of long time-series sampling of global rivers to elucidate controlling processes needed to quantify patterns in global silicate weathering rates.展开更多
Based on the digital seismic waveform data observed from regional and reservoir seismic networks,the seismicity and source parameters in the Zipingpu reservoir area from January 1,2000 to May 11,2008 before the Wenchu...Based on the digital seismic waveform data observed from regional and reservoir seismic networks,the seismicity and source parameters in the Zipingpu reservoir area from January 1,2000 to May 11,2008 before the Wenchuan MS8.0 earthquake are studied. The analysis shows a continuous activity of small earthquakes before the Wenchuan MS8. 0 earthquake. The level of seismic activity was from ML2.0 to ML3.0 in recent years. The impoundment of Zipingpu reservoir started in September 2005 and the obvious increment of seismic activities occurred in February 2008,but according to the analysis of the longer time series,the frequency and intensity of seismic activities do not exceed its range,which means it is still a fluctuation of regional seismic activities or tectonic activity. At the same time,we also studied the spatio-temporal distribution and the change of apparent stress difference in the source region before the great earthquake,and the results show that the apparent stress values of small earthquakes are generally higher than fitted values of apparent stress in the reservoir area in the nearly three months before the Wenchuan MS8.0 earthquake. It can be seen from the spatial distribution of apparent stress difference,that the epicenter of the earthquake is located at a low stress distribution area,and the relatively high apparent stress difference is at the east of the epicenter. Apparent stress increment occurred in local areas.展开更多
The paper makes a comparative review and evaluation of different theories on impacts of the distribution of labor and capital income on economic growth as well as criticism of the mainstream theories and suggests usin...The paper makes a comparative review and evaluation of different theories on impacts of the distribution of labor and capital income on economic growth as well as criticism of the mainstream theories and suggests using Bhaduri-Marglin model as the theoretical basis for relevant research. By formulating the econometric model and analyzing time series data from 1978 to 2012, the paper makes an empirical research into the impact of labor share on economic growth in China. It has discovered that the positive effect of labor share on consumption demand growth in China outweighs its negative effect on investment and net export growth, concluding that growth in China is wage-led at present. That may mean that (1) increasing labor share promotes growth and the goals of equality and efficiency supports each other," (2) the demand and optimizing structure" lies in regulation tools. key to "stabilizing growth, expanding domestic establishing a set of pro-labor institution and展开更多
The symbolic representation of time series has attracted much research interest recently. The high dimensionality typical of the data is challenging, especially as the time series becomes longer. The wide distribution...The symbolic representation of time series has attracted much research interest recently. The high dimensionality typical of the data is challenging, especially as the time series becomes longer. The wide distribution of sensors collecting more and more data exacerbates the problem. Representing a time series effectively is an essential task for decision-making activities such as classification, prediction, and knowledge discovery. In this paper, we propose a new symbolic representation method for long time series based on trend features, called trend feature symbolic approximation (TFSA). The method uses a two-step mechanism to segment long time series rapidly. Unlike some previous symbolic methods, it focuses on retaining most of the trend features and patterns of the original series. A time series is represented by trend symbols, which are also suitable for use in knowledge discovery, such as association rules mining. TFSA provides the lower bounding guarantee. Experimental results show that, compared with some previous methods, it not only has better segmentation efficiency and classification accuracy, but also is applicable for use in knowledge discovery from time series.展开更多
The recently introduced multivariate multiscale sample entropy(MMSE)well evaluates the long correlations in multiple channels,so that it can reveal the complexity of multivariate biological signals.The existing MMSE a...The recently introduced multivariate multiscale sample entropy(MMSE)well evaluates the long correlations in multiple channels,so that it can reveal the complexity of multivariate biological signals.The existing MMSE algorithm deals with short time series statically whereas long time series are common for real-time computation in practical use.As a solution,we novelly proposed our dynamic MMSE(DMMSE)as an extension of MMSE.This helps us gain greater insight into the complexity of each section of time series,producing multifaceted and more robust estimates than the standard MMSE.The simulation results illustrated the feasibility and well performance in the brain death diagnosis.展开更多
基金Supported by the Specialized Research Fund for the Doctoral Program of Higher Education,China (No. 200804230015)the National High Technology Research and Development Program of China (863 Program) (No. 2006AA10A401)
文摘RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication, DNA repair, and RNA processing. However, the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level. We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins, Fc-vasa and Fc-PL10a, from the testes of Chinese shrimp, Fenneropenaeus chinensis. The two predicted amino acid sequences contain all the conserved motifs characterized by the DEAD-box family and several RGG repeats in the N-terminal regions. Homology and phylogenetic analyses indicate that they belong to the vasa and PLIO subfamilies. The three-dimensional structures of the two proteins were predicted with a homology modeling approach. Both core proteins consist of two tandem RecA-like domains similar to those of the DEAD-box RNA helicase. Using reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR we found that Fc-vasa was expressed specifically in the adult gonads. Transcription decreased in the ovary but increased in the testis during gonadal development. Fc-PL10a expression was widely distributed in the tissues we examined. Using in situ hybridization, we demonstrated that the Fc-vasa transcript is localized to the cytoplasm of the spermatogonia and oocytes. Thus, our results suggest that Fc-wasa plays an important role in germ-line development, and has utility as a germ cell lineage marker which will help to generate new insight into the origin and differentiation of germ cells as well as the regulation of reproduction in F. chinensis.
基金supported by a Stanford EDGE-STEM Fellowshipinitiated under NSF EAR-1254156 to Kate Maher and was also supported by the California Alliance Research Exchange NSF HRD-1306595 to C.Page Chamberlain
文摘Quantifying the functional relationships relating river discharge and weathering products places key constraints on the negative feedback between the silicate weathering and climate. In this study we analyze the concentration–discharge relationships of weathering products from global rivers using previously compiled time-series datasets for concentrations and discharge from global rivers. To analyze the nature of the covariation between specific discharge and concentrations, we use both a power law equation and a recently developed solute production equation. The solute production equation allows us to quantify weathering efficiency, or the resistance to dilution at high runoff, via the Damkohler coefficient. These results are also compared to those derived using average concentration–discharge pairs.Both the power law exponent and the Damkohler coefficient increase and asymptote as catchments exhibit increasingly chemostatic behavior, resulting in an inverse relationship between the two parameters. We also show that using thedistribution of average concentration–discharge pairs from global rivers, rather than fitting concentration–discharge relationships for each individual river, underestimates global median weathering efficiency by up to a factor of ~10. This study demonstrates the utility of long time-series sampling of global rivers to elucidate controlling processes needed to quantify patterns in global silicate weathering rates.
基金sponsored by the research project on reservoir earthquake monitoring and prediction technique (2008BAC38B03-02),China
文摘Based on the digital seismic waveform data observed from regional and reservoir seismic networks,the seismicity and source parameters in the Zipingpu reservoir area from January 1,2000 to May 11,2008 before the Wenchuan MS8.0 earthquake are studied. The analysis shows a continuous activity of small earthquakes before the Wenchuan MS8. 0 earthquake. The level of seismic activity was from ML2.0 to ML3.0 in recent years. The impoundment of Zipingpu reservoir started in September 2005 and the obvious increment of seismic activities occurred in February 2008,but according to the analysis of the longer time series,the frequency and intensity of seismic activities do not exceed its range,which means it is still a fluctuation of regional seismic activities or tectonic activity. At the same time,we also studied the spatio-temporal distribution and the change of apparent stress difference in the source region before the great earthquake,and the results show that the apparent stress values of small earthquakes are generally higher than fitted values of apparent stress in the reservoir area in the nearly three months before the Wenchuan MS8.0 earthquake. It can be seen from the spatial distribution of apparent stress difference,that the epicenter of the earthquake is located at a low stress distribution area,and the relatively high apparent stress difference is at the east of the epicenter. Apparent stress increment occurred in local areas.
文摘The paper makes a comparative review and evaluation of different theories on impacts of the distribution of labor and capital income on economic growth as well as criticism of the mainstream theories and suggests using Bhaduri-Marglin model as the theoretical basis for relevant research. By formulating the econometric model and analyzing time series data from 1978 to 2012, the paper makes an empirical research into the impact of labor share on economic growth in China. It has discovered that the positive effect of labor share on consumption demand growth in China outweighs its negative effect on investment and net export growth, concluding that growth in China is wage-led at present. That may mean that (1) increasing labor share promotes growth and the goals of equality and efficiency supports each other," (2) the demand and optimizing structure" lies in regulation tools. key to "stabilizing growth, expanding domestic establishing a set of pro-labor institution and
基金supported by the National High-Tech R&D Program(863)of China(Nos.2012AA012600,2011AA010702,2012AA01A401,and 2012AA01A402)the National Natural Science Foundation of China(No.60933005)the National Science and Technology of China(No.2012BAH38B04)
文摘The symbolic representation of time series has attracted much research interest recently. The high dimensionality typical of the data is challenging, especially as the time series becomes longer. The wide distribution of sensors collecting more and more data exacerbates the problem. Representing a time series effectively is an essential task for decision-making activities such as classification, prediction, and knowledge discovery. In this paper, we propose a new symbolic representation method for long time series based on trend features, called trend feature symbolic approximation (TFSA). The method uses a two-step mechanism to segment long time series rapidly. Unlike some previous symbolic methods, it focuses on retaining most of the trend features and patterns of the original series. A time series is represented by trend symbols, which are also suitable for use in knowledge discovery, such as association rules mining. TFSA provides the lower bounding guarantee. Experimental results show that, compared with some previous methods, it not only has better segmentation efficiency and classification accuracy, but also is applicable for use in knowledge discovery from time series.
基金supported by KAKENHI(Grant Nos.21360179,22560425)(JAPAN)supported by the Key Project of National Science Foundation of China(Grant Nos.11232005)The Ministry of Education Doctoral Foundation(Grant Nos.20120074110020)
文摘The recently introduced multivariate multiscale sample entropy(MMSE)well evaluates the long correlations in multiple channels,so that it can reveal the complexity of multivariate biological signals.The existing MMSE algorithm deals with short time series statically whereas long time series are common for real-time computation in practical use.As a solution,we novelly proposed our dynamic MMSE(DMMSE)as an extension of MMSE.This helps us gain greater insight into the complexity of each section of time series,producing multifaceted and more robust estimates than the standard MMSE.The simulation results illustrated the feasibility and well performance in the brain death diagnosis.