A method for determination of lycopene concentration in dog plasma wasestablished. Methods RP-HPLC was used; the mobile phase consisted of methanol-acetonitrile-methylenechloride (40:30:30, V/V) , the wavelength of de...A method for determination of lycopene concentration in dog plasma wasestablished. Methods RP-HPLC was used; the mobile phase consisted of methanol-acetonitrile-methylenechloride (40:30:30, V/V) , the wavelength of detection was 472 nm, the column temperature wasambient temperature, and the flow rate was 1.0 mL·min^(-1). Results The standard curve was linearin the range from 0.012 4 to 0.496 μg·mL^(-1) with r=0.9992. The average extraction recovery was97.6% +-4.2%. The intra-day and inter-day RSD were 1.52% -4.95% and 2.31% -7.38%, respectively.Conclusion This method is sensitive, rapid, reproducible, and of good selectivity for the analysisof lycopene in dog plasma.展开更多
Three kinds of methods for processing the data of the multi-wavelength pyrometer are presented in this paper and are named curve auto-search method, curve auto-regression method and neural network method. Tbe experime...Three kinds of methods for processing the data of the multi-wavelength pyrometer are presented in this paper and are named curve auto-search method, curve auto-regression method and neural network method. Tbe experimental results indicate that the calculated temperature and the spectral emissivity compared with the true target temperature and spectral emissivity have significant deviation using the curve auto-search and the curve auto-regression methods. However, the calculated temperature and the spectral emissivity with higher accuracy can be obtained using the neural network method.展开更多
文摘A method for determination of lycopene concentration in dog plasma wasestablished. Methods RP-HPLC was used; the mobile phase consisted of methanol-acetonitrile-methylenechloride (40:30:30, V/V) , the wavelength of detection was 472 nm, the column temperature wasambient temperature, and the flow rate was 1.0 mL·min^(-1). Results The standard curve was linearin the range from 0.012 4 to 0.496 μg·mL^(-1) with r=0.9992. The average extraction recovery was97.6% +-4.2%. The intra-day and inter-day RSD were 1.52% -4.95% and 2.31% -7.38%, respectively.Conclusion This method is sensitive, rapid, reproducible, and of good selectivity for the analysisof lycopene in dog plasma.
基金Sponsored by the National Natural Science Foundation of China(Grant No. 60377037)the Scientific Research Foundation of Harbin Institute of Technol-ogy (Grant No. HIT. 2002. 18)the Spaceflight Support Foundation.
文摘Three kinds of methods for processing the data of the multi-wavelength pyrometer are presented in this paper and are named curve auto-search method, curve auto-regression method and neural network method. Tbe experimental results indicate that the calculated temperature and the spectral emissivity compared with the true target temperature and spectral emissivity have significant deviation using the curve auto-search and the curve auto-regression methods. However, the calculated temperature and the spectral emissivity with higher accuracy can be obtained using the neural network method.
