研究了氧化石墨烯(Graphene Oxide,GO)修饰的色散拐点长周期光纤光栅(Dispersion-Turning-Point Long Period Fiber Grating,DTP-LPFG)传感器,分析了其光谱特性及对外部折射率的灵敏度特性。利用准连续KrF激光器在载氢单模石英光纤上制...研究了氧化石墨烯(Graphene Oxide,GO)修饰的色散拐点长周期光纤光栅(Dispersion-Turning-Point Long Period Fiber Grating,DTP-LPFG)传感器,分析了其光谱特性及对外部折射率的灵敏度特性。利用准连续KrF激光器在载氢单模石英光纤上制作周期约为136μm的DTP-LPFG,采用氢键结合的方式将GO涂覆于光栅表面,构成基于GO修饰的DTPLPFG传感器。实验结果表明:随着GO在DTP-LPFG表面的沉积,其双谐振峰的左峰发生蓝移,右峰发生红移,双峰间距增大;在1.33~1.38折射率范围内,该传感器在涂覆GO后,谐振双峰间距变化量的折射率灵敏度约为831.89nm/RIU,较未修饰GO的DTP-LPFG提高了1.05倍。展开更多
Site_specific mutagenesis has been widely used in molecular biology and biochemistry. The authors have developed a simple and easy method for site_specific mutagenesis of any genes on plasmids using long distance inve...Site_specific mutagenesis has been widely used in molecular biology and biochemistry. The authors have developed a simple and easy method for site_specific mutagenesis of any genes on plasmids using long distance inverse PCR in the presence of Pfu_DNA polymerase. The efficiency of this method is higher than 90% and the entire procedure can be performed just in one tube. No subcloning is needed. This method is especially useful for obtaining mutant genes on large plasmids such as Ti plasmids used for plant transformation.展开更多
文摘研究了氧化石墨烯(Graphene Oxide,GO)修饰的色散拐点长周期光纤光栅(Dispersion-Turning-Point Long Period Fiber Grating,DTP-LPFG)传感器,分析了其光谱特性及对外部折射率的灵敏度特性。利用准连续KrF激光器在载氢单模石英光纤上制作周期约为136μm的DTP-LPFG,采用氢键结合的方式将GO涂覆于光栅表面,构成基于GO修饰的DTPLPFG传感器。实验结果表明:随着GO在DTP-LPFG表面的沉积,其双谐振峰的左峰发生蓝移,右峰发生红移,双峰间距增大;在1.33~1.38折射率范围内,该传感器在涂覆GO后,谐振双峰间距变化量的折射率灵敏度约为831.89nm/RIU,较未修饰GO的DTP-LPFG提高了1.05倍。
文摘Site_specific mutagenesis has been widely used in molecular biology and biochemistry. The authors have developed a simple and easy method for site_specific mutagenesis of any genes on plasmids using long distance inverse PCR in the presence of Pfu_DNA polymerase. The efficiency of this method is higher than 90% and the entire procedure can be performed just in one tube. No subcloning is needed. This method is especially useful for obtaining mutant genes on large plasmids such as Ti plasmids used for plant transformation.