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基于长种子的二代测序序列找全比对算法
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作者 吴邪 刘欢 徐云 《计算机系统应用》 2022年第10期310-316,共7页
主流的二代测序序列找全比对算法采用种子扩展的方法,由于长种子索引存在空间开销大或检索时间长的问题,这类算法大多使用短种子而导致候选位置过多,增加了比对的时间成本.为此,提出一种基于长种子的找全比对算法,设计了一种空间开销低... 主流的二代测序序列找全比对算法采用种子扩展的方法,由于长种子索引存在空间开销大或检索时间长的问题,这类算法大多使用短种子而导致候选位置过多,增加了比对的时间成本.为此,提出一种基于长种子的找全比对算法,设计了一种空间开销低和检索时间适度的长种子哈希索引,其通过模运算限制哈希空间并使用布隆过滤器识别同一存储位置上的不同种子.长种子显著减少候选位置数量,从而降低验证阶段的时间开销.实验结果表明,在人类基因序列测序数据集上,该算法维持同等精度的同时比现有主流算法时间效率更高. 展开更多
关键词 二代测序 找全比对算法 种子扩展 布隆过滤器 长种子
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不同方法萃取长叶橙种子油品质的比较 被引量:1
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作者 孙谦 胡中海 +3 位作者 孙志高 盖智星 黄巧娟 郝静梅 《食品与发酵工业》 CAS CSCD 北大核心 2015年第8期229-233,共5页
柑橘种子含油量为38.9%~58.5%,经精炼后具有较高的营养价值和应用潜力。文中分析了超临界CO2流体萃取法、超声波辅助溶剂萃取法、榨油机冷榨和热榨所得长叶橙种子油脂的初始理化指标、自氧化能力、柠檬苦素类似物含量、脂肪酸组成等指标... 柑橘种子含油量为38.9%~58.5%,经精炼后具有较高的营养价值和应用潜力。文中分析了超临界CO2流体萃取法、超声波辅助溶剂萃取法、榨油机冷榨和热榨所得长叶橙种子油脂的初始理化指标、自氧化能力、柠檬苦素类似物含量、脂肪酸组成等指标。4种方法所得油脂都符合食用植物油卫生标准,但超临界CO2流体萃取所得油脂酸价明显高于其他方法(P〈0.05)。超临界CO2流体萃取的种子油中柠檬苦素类似物含量最高(1.89 mg/g)。采用的4种萃取方法所得油脂中其脂肪酸的主成分均为棕榈酸、硬脂酸、油酸和亚油酸,超临界CO2流体萃取的种子油中含有较高的二高-γ-亚麻酸。经贮藏能力测定,推算出冷榨油脂在20℃条件下可贮藏92.4d,超临界CO2流体萃取油脂贮藏时间最短为41.7 d。综合分析发现,现超临界CO2流体所得初始油脂品质最好,但需经降酸工艺提高其贮藏能力。 展开更多
关键词 叶橙种子 品质分析 超临界CO2流体 超声波辅助溶剂 冷榨 热榨
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Research on the Morphological Character and Germination Characteristics of Tamarix elongata Ledeb. Seeds 被引量:1
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作者 于军 姜喜 +2 位作者 白宝伟 焦培培 庞新安 《Agricultural Science & Technology》 CAS 2010年第11期50-51,58,共3页
[Objective] The aim was to study the germination behavior of Tamarix elongata Ledeb.under natural conditions.[Method] By using T.elongata as the experimental materials,the seed morphology and germination characteristi... [Objective] The aim was to study the germination behavior of Tamarix elongata Ledeb.under natural conditions.[Method] By using T.elongata as the experimental materials,the seed morphology and germination characteristics were observed and measured.[Result] ① T.elongata seed was 1 011.96 μm in length and 399.94 μm in width,and the thousand-seed weight was 38.66 mg;② the germination rate,germination energy,germination index and germination coefficient of T.elongata seeds were 95.56%,46.67%,20.25 and 56.86 respectively;③ there were no significant differences in morphology and germination characteristics among the three different geographical populations.[Conclusion] The present research will provide reference for the introduction and breeding of T.elongata as well as the protection of sand dune vegetation. 展开更多
关键词 Tamatix elongata Ledeb. SEED MORPHOLOGY GERMINATION
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Branching and growth of plantings in fifth year of a seedling seed orchard of Masson pine (Pinus massoniana Lamb.) 被引量:3
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作者 赖焕林 王章荣 江瑞荣 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第1期28-32,83,共5页
The features of branching and growth studied included height, diameter at breast height (DBH), total number of branches, annual height growth, annual branch elongation in the year of elongating, annual branch number f... The features of branching and growth studied included height, diameter at breast height (DBH), total number of branches, annual height growth, annual branch elongation in the year of elongating, annual branch number for four consecutive years, diameter of branches of different ages, and diameter of stem where branch-whorl originates. For features of total growth and overall branching, no significant differences were found between families, except for DBH. For annual features, no significant differences were found in annual stem height growth, annual branch elongation in the year of elongation and diameter of branches. In the last four years, differences in number of branches were not significant in the first two years but were significant in the last two year; differences in stem diameter where branch-whorls grow were significant for the four consecutive years. Trend of annual growth and branching features of families can be divided into three types as increasing type, stable type and fluctuating type. Most of families have an increasing type with respect of annual height growth and annual branch elongation, while most families belong to a fluctuating type with annual branch number. The results indicated that in the fifth year after planted in seedling seed orchard, differences between families were mostly insignificant. This result may have two main explanations: one is the growth rhyme in early ages of Masson pine, the other one is the complex paternal components to form the open-pollinated families. Family selection seemed to be not useful based on the result. It is suggested to select some of families in the nursery instead of to use all the families when establishing seedling seed orchards with open-pollinated families from plus-trees. 展开更多
关键词 Pinus massoniana Lamb. Branching GROWTH Seedling seed orchard
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Effects of Pb Stress on Seed Germination and Seedling Growth of Two Weed Species Seeds 被引量:3
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作者 刘俊华 姚志刚 《Agricultural Science & Technology》 CAS 2016年第12期2726-2731,共6页
Objective] The aim was to investigate the effects of Pb stress on seed germination and seedling growth of Chenopodium glaucum and Chenopodium serot-inum L. [Method] With C. glaucum and C. serotinum as the study object... Objective] The aim was to investigate the effects of Pb stress on seed germination and seedling growth of Chenopodium glaucum and Chenopodium serot-inum L. [Method] With C. glaucum and C. serotinum as the study objects, the ef-fects of different concentrations of Pb on their seed germination and seedling growth, as wel as on the leaf SOD, POD and CAT activity were studied primarily. [Result] The germination potential of both the two kinds of weed seeds increased first and then decreased with the increase of Pb concentration. The germination po-tential of C. serotinum and C. glaucum seeds reached the peak at Pb concentration of 25 and 50 mg/L, respectively. With the increase of Pb treatment concentration, the germination rate, germination index and vigor index of C. serotinum and C. glaucum seeds decreased, and their bud length and root length also reduced gradu-al y. ln overal , the leaf SOD, POD and CAT activity of C. glaucum and C. serot-inum_ seedlings increased first and then decreased with the increased treatment concentration of Pb. The leaf SOD and POD activity of C. glaucum and C. serot-inum seedlings reached the maximum at Pb concentration of 200 and 100 mg/L, respectively, and the leaf CAT activity reached the maximum at Pb concentration of 100 mg/L. [Conclusion] The two Chenopodium species had strong tolerance to Pb stress, and they could be used as alternative accumulator plants of soil Pb contami-nation. 展开更多
关键词 Pb stress WEED Seed germination Seedling growth
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Study on Vigor of Kenaf Seeds Stored with Different Packaging Methods for 31 Years 被引量:1
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作者 覃初贤 《Agricultural Science & Technology》 CAS 2016年第8期1933-1937,共5页
This study was conducted to investigate the effects of different packaging methods on seed germination and vigor under a long-term bank storage condition, selecting suitable packaging materials and methods, and compre... This study was conducted to investigate the effects of different packaging methods on seed germination and vigor under a long-term bank storage condition, selecting suitable packaging materials and methods, and comprehensively estimate changes in seed vigor of seeds stored with different packaging materials. Hibiscus cannabinus L. seeds were stored in aluminium box, seed box, glass bottle, POlyethylene film bag, sack and kraft paper bag as packaging materials in a, long-term bank and tested for their seeds germinability, electrical conductivity and field seedling rate after 31 years. The results showed that the kenaf seeds with an initial germination rate of 88% exhibited the germination rates over 79.7% after being stored in the 6 packaging materials for 31 years, the germination rates of seeds packaged with paper bag and sack and of those sealed in film bag and aluminium box showed significantly decreased germination rates, while the seeds sealed in seed boxes and glass bottles exhibited non-significant decreases in seed germination rate, and their germination rates were still above 85%. Seed box and glass bottle were the packaging materials most suitable for long-term safe storage of kenaf seeds with a safe storage period long than 31 years. 展开更多
关键词 Kenaf seed Long-term storage Packaging materials GERMINATION VIGOR
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Effects of Cadmium Stress on Seed Germination and Seedling Growth of Brassica napus L. 被引量:1
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作者 郑本川 李浩杰 +5 位作者 张锦芳 柴靓 蒲晓斌 蒋俊 崔成 蒋梁材 《Agricultural Science & Technology》 CAS 2017年第4期591-595,601,共6页
To investigate the effects of the heavy metal cadmium (Cd) on seed ger- mination and seedling growth of Brassica napus L., 25 B. napus lines with different genetic background were treated with four concentrations (... To investigate the effects of the heavy metal cadmium (Cd) on seed ger- mination and seedling growth of Brassica napus L., 25 B. napus lines with different genetic background were treated with four concentrations (0, 1, 5 and 10 mg/L) of Cd, and then, their germination potential, germination rate, seedling length, main root length, seedling fresh weight, and seedling dry weight were measured. The re- sults showed that the response of the B. napus lines to Cd stress was different. With the increase of Cd concentration, the relative germination potential and relative seedling rate increased first and then decreased, and reached the maximum levels when the Cd concentration was 5 rag/L. The inhibitory effects of Cd stress on seedling growth indices in a decreasing order were root length〉seedling length〉 seedling fresh weight〉seedling water content. There were extremely significant differ- ences in the relative root length, relative seedling length, relative seedling fresh weight and relative seedling water content among the B. napus lines treated by dif- ferent concentrations of Cd, while there were no significant differences in the rela- tive germination potential and relative seedling rate among the lines, except that the relative seedling rate was significantly different among the lines treated by 5 mg/L Cd. The results suggested that seedling growth was more sensitive than seed ger- mination to heavy metal stress. 展开更多
关键词 Brassica napus. L Seed germination Seedling growth CADMIUM
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Growth and Influencing Factors of Three Native Tree Species in Coastal Casuarina equisetifolia Plantation
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作者 姚宝琪 刘强 +1 位作者 蔡梓 王孝杉 《Agricultural Science & Technology》 CAS 2011年第2期228-232,共5页
[Objective] The aim was to study the growth and environment factors of three native tree species(Thespesia lampas,Calophyllum inophyllum and Hernandia sonora)in Hainan in coastal Casuarina equisetifolia plantation.[... [Objective] The aim was to study the growth and environment factors of three native tree species(Thespesia lampas,Calophyllum inophyllum and Hernandia sonora)in Hainan in coastal Casuarina equisetifolia plantation.[Method] The coastal sandy soil was treated by adding wood chips,bagasse and the control without adding anything in the plant pits.The growth of three native tree species planted for one year was analyzed.[Result] ① One year later,the survival rate showed an order of T.lampasH.sonoraC.inophyllum;the net increase of basal diameter showed an order of H.sonoraT.lampasC.inophyllum;while the net increase of plant height showed an order of T.lampasC.inophyllumH.sonora.The difference of survival rate between T.lampas and C.inophyllum was extremely significant,the difference of survival rate between H.sonora and C.inophyllum was significant,but the survival rate between T.lampas and H.sonora showed no significant difference.The difference on net growth of plant height among T.lampas,C.inophyllum and H.sonora was extremely significant,and the different on the net growth of basal diameter between T.lampas and C.inophyllum was significant.② Among different soil treatments,the survival rate of three native tree species in the treatment by adding wood chips,bagasse were higher than the treatment without adding anything,and the difference of survival rate of T.lampas between the treatment by adding wood chips,bagasse and the treatment without adding anything was extremely significant,but the treatment by adding wood chips and bagasse had not significant difference.③ The growth of three native tree species showed some correlation with environment factors(∣r∣0.3).The survival rate of T.lampas showed higher correlation with soil pH value and soil moisture,and the basal diameter and height of T.lampas showed some correlation with soil pH value and soil temperature.The survival rate of C.inophyllum showed higher correlation with soil pH value,soil temperature and light intensity,while the basal diameter and height of C.inophyllum showed some correlation with soil temperature.The survival rate of H.sonora showed higher correlation with soil salinity and soil temperature,while the basal diameter and height of H.sonora showed some correlation with soil temperature.The basal diameter and height of three native tree species had higher correlation(r0.5),which had reached a significant level.[Conclusion] In the one-year growth period in C.equisetifolia forest,the native tree species of T.lampas grew best and it was the most potential forest species mixed with C.equisetifolia. 展开更多
关键词 Native tree species GROWTH Environment factors
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Construction and Quality Analysis of Full-length cDNA Library of Phyllostachys heterocycla Germinating Seeds
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作者 胡陶 姚娜 +2 位作者 杨学文 彭镇华 李潞滨 《Agricultural Science & Technology》 CAS 2013年第1期1-5,25,共6页
[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to constru... [Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments (1.0-5.0 kb) is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants. 展开更多
关键词 Phyllostachys heterocycla Full-length cDNA library Germinating seeds Oligo-capping method
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Effect of trace elements on growth of Pinus tabulaeformis seedling 被引量:7
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作者 刘晓东 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第4期285-288,337,共4页
The stimulative effect of trace elements on seed germination and seedling growth of Pinus tabulaeformis was tested. The experiments were carried out on seed soak and topdressing with different trace elements and varie... The stimulative effect of trace elements on seed germination and seedling growth of Pinus tabulaeformis was tested. The experiments were carried out on seed soak and topdressing with different trace elements and varied concentrations at the nursery of Gardens Research Institute, Harbin, in 2000-2001. The experimental results showed that soaking seed with 1% and 0.2% concentrations of Mn element produced best result for seed germination, and the germination rate was increased by 9%~19% for the seeds treated with 1% concentration and 12%~14% for the seeds treated with 0.2% concentration compared with the control group. The seeds treated with boron element had lowest germination rate. For trace element topdressing, Mn and Mo elements presented good result for seedling growth and the treatment with low concentration was even better. The height or chlorophyll content of the seedlings with spray of low-concentration Mn and Mo element was much higher than that of untreated ones. In the contrast to the treating method of seed soak, topdressing (application of spraying on foliage) had evident effect on seedling growth. 展开更多
关键词 Pinus tabulaeformis Trace element Seed soaking TOPDRESSING Seed germination Seedling growth
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Effects of Liquid Seaweed Bio-organic Fertilizer on Seed Germination and Seedling Growth of Vegetables 被引量:1
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作者 刘培京 王飞 张树清 《Agricultural Science & Technology》 CAS 2014年第3期407-411,共5页
[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seawe... [Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seaweed bio-organic fertilizer were prepared for seed soaking and pot incubation of cucumber, tomato and chili, to ob- serve the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of vegetables. [Result] Compared with the control, germination rate of cucumber and tomato seeds applied with 600-fold liquid seaweed bio-organic fer- tilizer varied significantly; germination rate of chili seeds applied with 400-fold liquid seaweed bio-organic fertilizer varied significantly; germination energy and germination index of chili seeds applied with different dilution concentrations of liquid seaweed bio-organic fertilizer presented no significant differences. In addition, 200-fold and 400-fold liquid seaweed bio-organic fertilizer significantly improved the root length, plant height, plant fresh weight, plant dry weight, chlorophyll content and leaf area of cucumber, tomato and chili seedlings; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content and leaf area of cucumber seedlings varied significantly compared with the control, but no significant differences were observed in plant height, plant fresh weight and plant dry weight; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content, plant height, plant fresh weight and plant dry weight of chili and tomato varied sig- nificantly compared with the control, but no significant differences were observed in leaf area. [Conclusion] Soaking vegetable seeds with liquid seaweed bio-organic fer- tilizer can significantly improve seed generation rate and seedling growth. 展开更多
关键词 Liquid seaweed bio-organic fertilizer CUCUMBER Chili Tomato Seed germination Seedling growth
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Effects of Bamboo Vinegar on Seed Germination and Seedling Growth of Different Flue-cured Tobacco Varieties
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作者 许灵杰 杨双剑 +2 位作者 董民 史元元 杜相革 《Agricultural Science & Technology》 CAS 2015年第8期1660-1665,共6页
[Objective] The present experiment was conducted to study the effects of bamboo vinegar on seed germination and seedling growth of different flue-cured to- bacco varieties, so as to provide some references for its app... [Objective] The present experiment was conducted to study the effects of bamboo vinegar on seed germination and seedling growth of different flue-cured to- bacco varieties, so as to provide some references for its application in tobacco pro- duction. [Method] By using indoor culture dish method and greenhouse seedling- raising plate culture,four different concentrations of bamboo vinegar dilution (50, 100, 200 and 400 times)were designed and clean water was used as the control (CK). The germination indices of Yunyan 97, Guiyan 2, Jiucaiping 2 and Bina 1 were de- termined after seed germinating. The seedling growth indices of Yunyan 97 were investigated after 10 days of spaying bamboo vinegar on seedling leaves at the 3r~ time. [Result] Compared with CK, different bamboo vinegar dilutions could inhibit the seed germination and seedling growth of all flue-cured tobacco varieties. The seed germination rate and index, vigor index, whole length, root length, stem length and fresh weight of seedlings of all varieties presented decreasing trend to varying de- grees, moreover, most of them reached significant level. Compared with CK, spray- ing different concentrations of bamboo vinegar dilution at the seedling stage could improve the quality of seedlings. In all bamboo vinegar treatments, the plant height, the maximum leaf length and root volume under 100 times dilution treatment were the highest with 3.36 and 10.14 cm, and 0.92 ml, respectively. And the stem girth, dry weight and the maximum leaf width of seedlings under 400 times dilution treat- ment also showed the highest with 1.82 cm, 0.262 g and 5.74 cm, respectively. [Conclusion] Supplying different concentrations of bamboo vinegar solution on culture dish could inhibit the seed germination and seedling growth of flue-cured tobacco, while spraying 100-400 times bamboo vinegar solution to leaves could promote the seedling growth and improve the quality of Yunyan 97 to varying degrees. 展开更多
关键词 Bamboo vine clar Flue-cured tobacco Seed germination Seedling growth
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Effect of Different Treatments on the Germination and Seedling Growth of Firmiana simplex
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作者 翟书华 殷根深 +1 位作者 杨晓虹 刘开庆 《Agricultural Science & Technology》 CAS 2016年第6期1367-1370,共4页
With Firmiana simplex (L.) W. F. Wight as an experiment material, the effects of different concentrations of GA3 (Gibberellin A3), SA (Salicylic acid) and NAA (1-Naphthalene acetic acid) and stratification on ... With Firmiana simplex (L.) W. F. Wight as an experiment material, the effects of different concentrations of GA3 (Gibberellin A3), SA (Salicylic acid) and NAA (1-Naphthalene acetic acid) and stratification on the germination rate of seeds were studied firstly, and then the effects of GA3 and NAA on seedling growth were analyzed. The results showed that the germination rate could be improved by 0.075 g/L SA and stratification markedly. It was indicated by the experiment that the growth of the main root and main stem could be promoted by 0.050 g/L and 0.077 g/L NAA, respectively. However, the leaf length and leaf width decreased significantly in the GA3 and NAA treatments. 展开更多
关键词 Firmiana simplex Germination rate Seedling growth STRATIFICATION HORMONE
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Study on Cadmium Tolerance of Seed Germination and Seedlings Growth of Different Rice Varieties
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作者 孟桂元 李梦阳 +3 位作者 周静 唐婷 肖珑 曾淑英 《Agricultural Science & Technology》 CAS 2015年第11期2451-2455,2461,共6页
The research used bioassay method to explore seed tolerance of Cd in terms of seed germination and seedling growth. Specifically, 12 rice varieties of three types were investigated under Cd stress, covering germinatio... The research used bioassay method to explore seed tolerance of Cd in terms of seed germination and seedling growth. Specifically, 12 rice varieties of three types were investigated under Cd stress, covering germination rate, shooting index, vigor index, seedling and shoot lengths, and fresh weights. The results showed that as Cd concentration grew, seed germination and seedling growth kept increasingly inhibited; germination index, vigor index, root length, shoot length and fresh weights went down; inhibition effects on seed germination and seedling growth were conventional rice〉two-line hybrid rice〉three-line hybrid ,rice. Shanyou 9588, Tianyou 998, Chuanxiang 8, and Y Liangyou 1 performed ~rongly in Cd tolerance; C Liangyou 651, Nei 5 You 263, Xiangwanxian 16, Huanghuazhan, Xiangwanxian 13, and Zhunliangyou 527 performed poorly in Cd tolerance, and Cd tolerance of rest treatments were moderate. 展开更多
关键词 Variety types Rice seed GERMINATION Seedling growth Cadmium toterance
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Effects of Trichoderma harzianum YC_(459) and soil types on seed germination and seedling growth in rock slope restoration 被引量:2
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作者 MA Hoseop ETTAGBOR Hans Enukwa KIM Choonsig 《Journal of Mountain Science》 SCIE CSCD 2018年第4期730-737,共8页
We carried out experiments with various concentrations of Trichoderma harzianum YC459 in different soil types(forest soil, mixed soil,merchantable soil, and leaf mold soil) to evaluate its effect on seed germination a... We carried out experiments with various concentrations of Trichoderma harzianum YC459 in different soil types(forest soil, mixed soil,merchantable soil, and leaf mold soil) to evaluate its effect on seed germination and seedling establishment of four species(Festuca arundinacea Schreb., Dianthus barbatus var. asiaticus Nakai,Lespedeza cyrtobotrya Miq., and Parthenocissus tricuspidata Planch) for rock slope restoration. We also investigated the use of drilled slanted holes on the rock slopes for seedling establishment. The results showed that T. harzianum concentration had significant effects on seed germination, seedling growth, and seedling survival for all the species with different soil types. Seed germination and survival rates peaked at 5% T. harzianum concentration with leaf mold soil and decreased as T. harzianum concentration increased from 5% to 10%. Seedling survival rates of all four species were generally lowest at 0% T. harzianum concentration in all soil types.The height of F. arundinacea and L. cyrtobotrya peaked at 5% T. harzianum concentration whereas that of D. barbatus and P. tricuspidata peaked at 10%T. harzianum concentration. We concluded that 5% T. harzianum concentration with leaf mold soil is appropriate for seed germination and seedling survival rates of most species, thus enhancing seedling establishment. Practical application of the findings of this study will contribute in the vegetation restoration of steep rocks in mountain environments. 展开更多
关键词 Dianthus barbatus Drilled holes Festuca arundinacea Lespedeza cyrtobotrya Seedling establishment Seedling survival
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Anti-tumor activity of erlotinib in the BxPC-3 pancreatic cancer cell line 被引量:5
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作者 Ying-Ying Lu Da-Dao Jing +2 位作者 Ming Xu Kai Wu Xing-Peng Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第35期5403-5411,共9页
AIM:To investigate the effect and mechanism of action of erlotinib, an epidermal growth factor receptor (EGFR) small molecule tyrosine kinase inhibitor (TKI), in the human pancreatic cancer cell line BxPC-3 both ... AIM:To investigate the effect and mechanism of action of erlotinib, an epidermal growth factor receptor (EGFR) small molecule tyrosine kinase inhibitor (TKI), in the human pancreatic cancer cell line BxPC-3 both in vitro and in vivo.METHODS: In vitro, human pancreatic cancer cell line BxPC-3 was exposed to varying concentrations of ertotinib, and its effects on proliferation, cell cycle distribution, apoptosis and the expression of proand antiapoptotic factors such as bcl-2, bcl-xl, bax and bak, and the expression of vascular endothelial cell growth factor (VEGF) were measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, terminal deoxynucleotidyl transferase-mediated nick end labeling assay (TUNEL), and reverse transcriptionpolymerase chain reaction (RT-PCR). Potential effect of erlotinib on angiogenesis was examined by tube formation assay. Tumor growth suppression was observed in xenografted nude mice with pancreatic cancer in vivo. Immunohistochemical (IHC) staining for EGFR and factor VII-related antigen was undertaken to detect the microvessel density and VEGF expression in tumor tissue in xenograft nude mice.RESULTS: Erlotinib, as a single agent, repressed BxPC-3 cell growth in a dose-dependent manner, triggered G1 arrest and induced cell apoptosis, and suppressed capillary formation of endothelium in vitro. Expressions of VEGF were significantly down-regulated at a high concentration of 200 μmol/L, however, the expressions of bcl-2 and bcl-xl were decreased at 50 μmol/L. In vivo, Erlotinib-treated mice demonstrated a reduced tumor volume, weight and microvessel density as compared to the control. IHC staining showed decreased expression of EGFR and RT-PCR had lower VEGF expression in treated mice.CONCLUSION: The in vitro and in vivo findings provide evidence that BxPC-3 cells are inhibited with erlotinib treatment. Inhibition of EGFR may be a promising adjuvant chemotherapy strategy in pancreatic cancer treatment. 展开更多
关键词 Pancreatic cancer ERLOTINIB Epidermal growth factor receptor Human xenograft model ANGIOGENESIS
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Effects of the anti-tumor composition ofthe acetoacetate extract of vitex negundoseed on the growth of human cervical carcinomaxenografts in nude mice 被引量:2
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作者 Yanlin Cai Zhongdong Chen +3 位作者 Aiqiong Tang Bin Jiang Zhaohua Fan Jun Bai 《The Chinese-German Journal of Clinical Oncology》 CAS 2014年第3期105-109,共5页
Objective: The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed (EVn-50) on the growth of human cervical carcinoma HeLa cells xenograft... Objective: The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed (EVn-50) on the growth of human cervical carcinoma HeLa cells xenografts in nude mica and its possible molecular mechanism. Methods: Models of human cervical cancer HeLa cells xenografts transplanted subcuta- neously in nude mice were established and randomly divided into 7 groups (each group including 5 nude mice): saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xe- nograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels ofLDH, ALT, Cr and WBC counts were examined and compared. The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blot- ting. Results: EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant effect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conclusion: EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its pro- motion on xenografts cells apoptosis through down-regulation of Bcl-2 expressionPand activation of P53 expression. 展开更多
关键词 cervical cancer EVn-50 XENOGRAFTS apoptosis
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Allelopathic Potentials of Some Crop Residues on the Germination and Growth of Bidens pilosa. L. 被引量:3
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作者 A. J. Modupe K. Joshua T. P. Olugbenga 《Journal of Agricultural Science and Technology》 2010年第1期21-24,40,共5页
The allelopathic potentials of the aqueous extracts of rice husk and sorghum stem were examined on the germination and growth ofBidens pilosa. L. The extracts inhibited the germination ofB. pilosa seeds and the growth... The allelopathic potentials of the aqueous extracts of rice husk and sorghum stem were examined on the germination and growth ofBidens pilosa. L. The extracts inhibited the germination ofB. pilosa seeds and the growth lengths of its radicle and plumule. In both extracts, the severity of the inhibition increased with the increase in the concentrations of the extracts, indicating that the effects of the extracts were concentration dependent. The results obtained also showed that the degree of retardation appeared to be more pronounced in the rice husks extract-treated seeds than those of sorghum extract-treated seeds. However there were no significant differences (at 5% level) in the comparison of the degree of inhibitions of radicle and plumule growths at the varying extract concentrations as well as those of the control in both extracts treated seeds. 展开更多
关键词 ALLELOPATHY ALLELOCHEMICALS Bidens pilosa SORGHUM rice husk weed infestation.
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Phenological Observation of Man-made Phoebe bournei Forests 被引量:1
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作者 陈建毅 《Agricultural Science & Technology》 CAS 2014年第4期706-708,共3页
[Objective] The aim was to research annual shoot increments, blooming and fruit-bearing times, and sprouting ratio of man-made Phoebe boumei.[Methed] Phenological observations of Phoebe boumei blooming were conducted ... [Objective] The aim was to research annual shoot increments, blooming and fruit-bearing times, and sprouting ratio of man-made Phoebe boumei.[Methed] Phenological observations of Phoebe boumei blooming were conducted as per method of phytocoenology. [Result] Phoebe bournei shot three times per year, and autumn shoots proved the largest, followed by summer shoots and spring sprouts. Sprouts shaped from late April to early May, and bloomed from late April to middle May; young fruits grew from middle May to late May and matured from middle De- cember to late December. Thousand-seed weight of fruits reached 487-503 g, and averaged 496 g; thousand-seed weight of seed was 265-275 g and averaged 269 g; sprouting rate of seed in room was in 84.8%-88.5% and averaged 86.5%; sprouting rate of seeds in fields was in 68.4%-72.6%, and averaged 70.4%. [Conclusion] The research provides references for man-made Phoebe bournei forests. 展开更多
关键词 Phoebe boumei Shoot increment Blooming and bearing fruits Seeds
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Seed Germination and Seedling Growth of Idesia polycarpa 被引量:2
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作者 Xiaomei TAN Jianmei MAO +1 位作者 Xiaofeng AN Lihua LOU 《Agricultural Science & Technology》 CAS 2017年第1期98-100,106,共4页
[Objective] This study was conducted to characterize the seed germination and seedling growth of Idesia polycarpa. [Method] After 3-5 h of soaking in 5% baking soda solution, the waxy outer layer of the seeds was rubb... [Objective] This study was conducted to characterize the seed germination and seedling growth of Idesia polycarpa. [Method] After 3-5 h of soaking in 5% baking soda solution, the waxy outer layer of the seeds was rubbed off. Then, the seeds were mixed with fine sand and evenly sown on seedbeds. Germinated seeds were counted to calculate germination rate. Seedlings at two-or three-leef stage were transplanted, and their phenophases were observed and recorded. Height and ground diameter of the seedlings were monitored in the following three years. [Result] The seed germination rate was increased to 20% by soaking in 5% baking soda solution. The yearly seedling height growth of L polycarpa increased with the increase in seedling age. The monthly seedling height growth from August to October was much higher than in other months in the first year, and the growth in seedling height in the three months accounted for 79% of the total increase in that year. The seedling height grew rapidly from May to July in the following two years. In detail, the increase in seedling height from May to July accounted for 87.94% of the total increase in the second year, and 74.16% of the total increase in the third year. The monthly seedling height growth peaked in May in the two years. The yearly growth in ground diameter of I. polycarpa seedlings also increased with the increase in seedling age. The monthly growth in ground diameter from July to September was much higher than in other months in the first year, and the increase in ground diameter in the three months accounted for 80% of the total increase in that year. In the second and third years, the monthly growth in ground diameter from July to October was much higher than in other months. The increase in ground diameter in the two months accounted for 76.89% of the total increase in the second year, and 61.23% of the total increase in the third year. The monthly growth in ground diameter peaked in August in the two years. [Conclusion] The results will provide a theoretical basis for the development and popularization of L polycarpa in Chongqing and surrounding provinces and cities. 展开更多
关键词 Idesia-polycarpa Seed germination Growth pattern PHENOPHASE
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