长管体垂直侵彻半无限均质靶板的数值模拟

利用Johnson Cook材料模型与Grunissen状态方程 ,对长管体侵彻半无限均质靶板进行了数值模拟 ,并与实验进行了比较 .结果表明 ,数值模拟结果与实验结果具有较好的一致性 。

长管体斜侵彻有限厚均质靶板简化模型

进行了长管体斜侵彻有限厚靶板的试验．建立了长管体斜侵彻有限厚均质靶板的简化模型,描述了侵彻的物理图像,对长管体的受力和运动情况进行了分析．弹坑的剖面形状与长杆体斜侵彻有限厚靶板极为相似,而在正面及背面弹坑形状出现了明显的两个‘犄角”．计算结果表明,在火炮初速范围内,长管体的剩余速度随撞击速度的增加近似为线性递增．试验与计算的极限穿透速度吻合较好,说明所建立的模型能够描述侵彻过程．

长管体斜侵彻半无限均质靶简化模型

长管体斜侵彻半无限靶的研究具有重要意义。描述了长管体在速度范围在1000m/s^1500m/s时斜侵彻半无限均质靶板的物理图像,对长管体在侵彻各个阶段的受力和运动情况进行了分析,发现侵彻时管体中央的“靶芯”对侵彻过程影响很大。在一定假设条件下,基于变质量系统动量守恒定律建立了不同侵彻阶段长管体侵彻的质心运动方程,基于动量矩定理建立了不同侵彻阶段绕质心的摆动方程,并进行了数值计算。进行了长管体侵彻半无限均质靶板的试验,试验与计算结果表明二者的侵彻行程吻合较好,说明所建立的模型能够描述长管体对半无限均质靶板的侵彻过程,对空心弹和异形穿甲弹的研究和设计具有一定参考价值。

长管体斜侵彻有限厚均质靶板数值模拟

长管体侵彻靶板的研究具有重要意义。文中结合长管体斜侵彻有限厚靶板的试验,利用三维非线性动力学有限元程序LS-DYNA3D,建立了大倾角下长管体斜侵彻有限厚靶板的有限元分析模型,描述了侵彻全过程的物理和力学现象,并与试验进行了对比,数值结果与试验结果吻合较好,对进一步研究伸出式侵彻体的侵彻能力有一定的参考价值。

有攻角长管体垂直侵彻半无限厚靶的简化模型

描述了带攻角长管体垂直侵彻半无限厚靶的物理图像,分析了“靶芯”力的大小和方向,建立了侵彻简化模型;给出了在不同的初始攻角条件下,管体在侵彻过程中攻角和穿深的变化规律.相同速度下,随着初始攻角的增大侵彻深度变小,基本呈线性关系.计算结果与实验结果吻合较好.

NaI(Tl)对6.13MeV-γ射线的长管体源的探测效率的测量

导出了核动力反应堆一回路16N的泄漏率与γ射线探测器的计数率、高压蒸气流速、16N的衰变常量及其发射γ射线的概率和效率的关系式,并得出效率与高压蒸气流速和16N的衰变常量等无关的结论.用发射6.13MeV-γ射线的PuC点源测量了NaI(Tl)探测器对长管体源的相对效率的空间分布和参考点的绝对效率,据此计算出长管体源的效率,效率的测量值与MC法的计算值在7%之内符合.分析了6.13和7.12MeVγ射线的效率差异对16N泄漏率和Compton小角散射的次级γ射线对收敛长度的影响.

Real-time Quantitative PCR Analysis of Vascular Endothelial Growth Factor Receptor-2(VEGFR-2) Expression at Zebrafish Different Developmental Stages

[Objective]To investigate the expression of zebrafish vascular endothelial growth factor-2（VEGFR-2） at different developmental stages.[Method]Total RNAs were extracted from 12,24,48,72 and 96 hpf stage zebrafish embryos and larvae.Real-time quantitative RT-PCR was performed to examine the expression of VEGFR-2.The data were analyzed by 2^-△△Ct method.[Result]The expression level of VEGFR-2 gene increased gradually from 12 to 72 hpf,and subsequently decreased at 96 hpf.The expression level was lowest at 12 hpf,highest at 72 hpf,and had significant differences when compared with that of other developmental stages.[Conclusion]The expression level of VEGFR-2 increases gradually before blood vessel maturation and decreases as blood vessels mature.

Effects of Ghrelin Antisense Inhibition on VEGF and Its Receptor Flt-1 mRNA Expression

[ Objective] This study was to investigate the effect of VEGF and its receptor Fit-1 mRNA expression in Mongolia sheep umbilical vein endothelial cells by ghrelin antisense inhibition. [ Method] Experiments were divided into 4 groups： group Ⅰ （blank control group） ; group Ⅱ （liposome group） ; group Ⅲ （SCON group： 20 μmol/L sense oligonucleotide） ; group Ⅳ （ASCON： 20 μmol/L antisense oligonucleotide）. VEGF and its receptor Fit-1 mRNA expression changes were detected by using real-time fluorescence quantitative detection after 24, 36 and 48 h. [ Result] The expression of VEGF mRNA in group Ⅰ, group Ⅱ were insignificantly different at higher expression levels, and did not change significantly with the time; the expression of VEGF mRNA in group Ⅲ assumed a slight decrease, but there were no significant differences between group I and group Ⅱ （P 〉0.05）, the expression of VEGF mRNA in group Ⅳ（antisense oligonucleotide group ） decreased significantly （P 〈 0.05） ; the expression of VEGF receptor FLT-1 mRNA was similar to that of VEGF. [ Conclusion] Antisense inhibition ghrelin has a downward effect to the expression of VEGF and its receptor Fit-1 the mRNA.

Effects of endostatin on expression of vascular endothelial growth factor and its receptors and neovascularization in colonic carcinoma implanted in nude mice

AIM:To investigate the antiangiogenic effects of endostatin on colonic carcinoma cell line implanted in nude mice and its mechanism. METHODS:Nude mice underwent subcutaneous injection with LS-174t colonic carcinoma cell line to generate carcinoma and were randomly separated into two groups.Mice received injection of vehicle or endostatin every day for two weeks. After the tumor was harvested,the tumor volumes were determined,and the expressions of CD34,VEGF and FIk-1 were examined by immunohistochemical method. RESULTS:Tumor volume was significantly inhibited in the endostatin group(84.17%)and tumor weight was significantly inhibited in the endostatin group(0.197±0.049) compared to the control group(1.198±0.105)(F=22.56, P=0.001),microvessel density(MVD)was significantly decreased in the treated group(31.857±3.515)compared to the control group(100.143±4.290)(F=151.62,P<0.001). Furthermore,the expression of FIk-1 was significantly inhibited in the treated group(34.29%) ompared to the control group(8.57%)(X^2=13.745,P=0.001).However no significant decrease was observed in the expression of vascular endothelial growth factor(VEGF)between these two groups(X^2=0.119,P=0.730). CONCLUSION:Endostatin can inhibit tumor growth and angiogenesis by blocking Vegf/FIk-1 pathway.This experiment provides the theory basis for developing a new anti-carcinoma drug through studying the properties of anti-angiogenesis inhibitors.

Signaling pathway/molecular targets and new targeted agents under development in hepatocellular carcinoma

Advances in molecular cell biology over the last de- cade have clarified the mechanisms involved in can- cer growth, invasion, and metastasis, and enabled the development of molecular-targeted agents. To date, sorafenib is the only molecular-targeted agent whose survival benefit has been demonstrated in two global phase 111 randomized controlled trials, and has been approved worldwide. Phase 111 clinical trials of other molecular targeted agents comparing them with sorafenib as first-line treatment agents are ongoing. Those agents target the vascular endothelial growth factor, platelet-derived growth factor receptors, as well as target the epidermal growth factor receptor, insulin- like growth factor receptor and mammalian target of rapamycin, in addition to other molecules targeting other components of the signal transduction pathways. In addition, the combination of sorafenib with standard treatment, such as resection, ablation, transarterial em- bolization, and hepatic arterial infusion chemotherapy are ongoing. This review outlines the main pathways involved in the development and progression of hepato- cellular carcinoma and the new agents that target these pathways. Finally, the current statuses of clinical trials of new agents or combination therapy with sorafenib and standard treatment will also be discussed.

Targeted medical therapy of biliary tract cancer:Recent advances and future perspectives

The limited efficacy of cytotoxic therapy for advanced biliary tract and gallbladder cancers emphasizes the need for novel and more effective medical treatment options. A better understanding of the specific biological features of these neoplasms led to the development of new targeted therapies, which take the abundant expression of several growth factors and cognate tyrosine kinase receptors into account. This review will briefly summarize the status and future perspectives of antiangiogenic and growth factor receptor-based pharmacological approaches for the treatment of biliary tract and gallbladder cancers. In view of multiple novel targeted approaches, the rationale for innovative therapies, such as combinations of growth factor (receptor)-targeting agents with cytotoxic drugs or with other novel anticancer drugs will be highlighted.

Effects of Gas Flow Field on Clogging Phenomenon in Close-Coupled Vortical Loop Slit Gas Atomization

In order to study the basic characteristics of gas flow field in the atomizing chamber near the nozzle outlet of the vortical loop slit atomizer and its influence mechanism on clogging phenomenon,the computational fluid dynamics(CFD)software Fluent is used to conduct a numerical simulation of the gas flow field in the atomizing chamber near the nozzle outlet of this atomizer under different annular slit widths,different atomization gas pressures and different protrusion lengths of the melt delivery tube. The results show that under atomization gas pressure p=4.5 MPa,the greater the annular slit width D,the lower the static temperature near the central hole outlet at the front end of the melt delivery tube,and the smaller the aspirating pressure at the front end of the melt delivery tube. These features can effectively prevent the occurrence of the clogging phenomenon of metallic melt. Under an annular slit width of D=1.2 mm,when the atomization gas pressure satisfies 1 MPa ≤ p ≤ 2 MPa and increases gradually,the aspirating pressure at the front end of the melt delivery tube will decline rapidly. This can prevent the clogging phenomenon of metallic melt. However,when the atomization gas pressure p >2 MPa,the greater the atomization gas pressure,the lower the static temperature near the central hole outlet at the front end of the melt delivery tube,and the greater the aspirating pressure at the front end of the melt delivery tube. Hence,the effect of preventing the solidification-induced clogging phenomenon of metallic melt is restricted. When atomization gas pressure is p =4.5 MPa and annular slit width is D=1.2 mm,the greater the protrusion length H of the melt delivery tube,and the smaller the aspirating pressure at its front end. The static temperature near the central hole that can be observed in its front end is approximate to effectively prevent the occurrence of clogging phenomenon of metallic melt. However,because of the small aspirating pressure,the metallic melt flows into the atomizing chamber from the central hole at the front end of the melt delivery tube at an increasing speed and the gas-melt ratio in the mass flow rate is reduced,which is not conducive to the improvement of atomization performance.

Cyclooxygenase-2 and epithelial growth factor receptor up-regulation during progression of Barrett's esophagus to adenocarcinoma

AIM： To investigate the expression of cyclooxygenase-2 （COX-2） and epithelial growth factor receptor （EGFR） throughout the progression of Barrett＇s esophagus （BE）. METHODS： COX-2 and EGFR protein expressions were detected by using immunohistochemical method. A detailed cytomorphological changes were determined. Areas of COX-2 and EGFR expression were quantified by using computer Imaging System. RESULTS： The expressions of both COX-2 and EGFR increased along with the progression from BE to esophagus adenocarcinoma （EAC）. A positive correlation was found between COX-2 expression and EGFR expression. CONCLUSION： COX-2 and EGFR may be cooperative in the stepwise progression from BE to EAC, thereby leading to carcinogenesis.

Gene transfer of somatostatin receptor type 2 by intratumoral injection inhibits established pancreatic carcinoma xenografts

AIM: To investigate the therapeutic effect of somatostatin receptor type 2 (SSTR2) gene transfection on pancreatic carcinoma xenografts in vivo in experimental cancers. METHODS: Human pancreatic cancer cell line Panc-1 was inoculated subcutaneously into the back of nude mice. When tumor nodules were grown as large as about 5 mmx5 mm days after inoculation, the mice were randomly divided into 3 groups (6 mice in each group). Group Ⅰ served as untreated control group. Group Ⅱ received an intratumoral injection of a combination of human cytomegalovirus promoter-6C (pCMV-6C) and lipofectamine 2000. Group Ⅲ received an intratumoral injection of a combination of pCMV-6C-SSTR2 and lipofectamine 2000. The rate of tumor growth was compared among these three groups. The expression of SSTR2 in these tumors was detected by immunohistochemistry and Western-blot. Apoptosis index (AI) in these tumors was examined by using TUNEL in situ. RESULTS: Intratumoral injection of a combination of pCMV-6C-SSTR2 and lipofectamine 2000 resulted in the expression of SSTR2 protein. The tumor size and weight in group Ⅲ (0.318±0.098 cm3, and 0.523±0.090 g, respectively) were significantly lower than those in group I (2.058±0.176 cms, and 1.412±0.146 g, respectively) and group Ⅱ (2.025±0.163 cm3, and 1.365±0.116 g, respectively) (P<0.05) The AI in group Ⅲ (1.47±0.13%) was significantly higher than that in groupⅠ(0.56±0.09%) and group Ⅱ (0.57±0.11%) (P<0.05). But there were no significant differences between groups Ⅰ and Ⅱ. CONCLUSION: Our data demonstrate that re-expression of SSTR2 gene has antitumor effects on experimental pancreatic cancer. Restoration of SSTR2 gene expression through gene transfer in vivo might be a potential gene therapy strategy for human pancreatic cancer.

Expression of thymidine kinase mediated by a novel non-viral delivery system under the control of vascular endothelial growth factor receptor 2 promoter selectively kills human umbilical vein endothelial cells

AIM: To investigate the killing efficiency of a recombinant plasmid containing a thymidine kinase (TK) domain insert driven by the vascular endothelial growth factor receptor 2 (VEGFR2) promoter (KDR) on vascular endothelial cells.METHODS: The KDR-TK fragment was extracted from pBluescript Ⅱ KDR-TK plasmid by enzymatic digestion with Xho I and Sal I. The enhanced green fluorescence protein (EGFP) carrier was extracted from pEGFP by the same procedure. The KDR-TK was inserted into the pEGFP carrier to construct pEGFP-KDR-TK. Using ultrasound irradiation and microbubble, pEGFP-KDR-TK was transferred into human umbilical vein endothelial cells (HUVECs). The transient infection rate was estimated by green fluorescent protein (GFP) expression. Transfected HUVECs, non-transfected HUVECs, and HepG2 cells were cultured in the presence of different concentrations of ganciclovir (GCV), and the killing efficacy of HSV-TK/GCV was analyzed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: The recombinant pEGFP-KDR-TK was successfully constructed by inserting the KDR-TK fragment into the pEGFP carrier. Transfected HUVECs showed cytoplasmic green fluorescence, and the transient transfection rate was about 20.3%. Pools of G418-resistant cells exhibited a higher sensitivity to theprodrug/GCV compared to non-transfected HUVECs or non-transfected HepG2 cells, respectively. CONCLUSION: KDR promoter and the suicide gene/prodrug system mediated by diagnostic ultrasound combined with microbubble can significantly kill HUVECs. Such therapy may present a novel and attractive approach to target gene therapy on tumor vessels.

Inhibition of tumor angiogenesis by TTF1 from extract of herbal medicine

AIM:To study the inhibition of tumor angiogenesis by 5,2,4'-trihydroxy-6,7,5'-trimethoxyflavone(TTF1) isolated from an extract of herbal medicine Sorbaria sorbifolia.METHODS:Angiogenic activity was assayed using the chick embryo chorioallantoic membrane(CAM) method.Microvessel density(MVD) was determined by staining tissue sections immunohistochemically for CD34 using the Weidner capillary counting method.The mRNA and protein levels of vascular endothelial growth factor(VEGF),vascular endothelialgrowth factor receptor 2(VEGFR2,Flk-1/KDR),basic fibroblast growth factor(bFGF),cyclo-oxygenase(COX)-2 and hypoxia-inducible factor(HIF)-1α were detected by quantitative real-time polymerase chain reaction and Western blotting analysis.RESULTS:The TTF1 inhibition rates for CAM were 30.8%,38.2% and 47.5% with treatment concentrations of 25,50 and 100 μg/embryo × 5 d,respectively.The inhibitory rates for tumor size were 43.8%,49.4% and 59.6% at TTF1 treatment concentrations of 5,10,and 20 μmol/kg,respectively.The average MVD was 14.2,11.2 and 8.5 at treatment concentrations of 5 μmol/kg,10 μmol/kg and 20 μmol/kg TTF1,respectively.The mRNA and protein levels of VEGF,KDR,bFGF,COX-2 and HIF-1α in mice treated with TTF1 were significantly decreased.CONCLUSION:TTF1 can inhibit tumor angiogenesis,and the mechanism may be associated with the down-regulation of VEGF,KDR,bFGF,HIF-1α and COX-2.

Measuring and comparing in-situ CO2 and CO profiles with satellite observations and model data

Accurate measurements of the vertical distribution of carbon dioxide(CO2)in the atmosphere are critical to validate the vertical columns recorded by satellite and ground-based spectrometers and to estimate sources and sinks of CO2.This work describes an in-situ air-sampler system(Aircore).Profiles of CO2 and carbon monoxide(CO)were retrieved from two soundings of this system in Xilin Hot on 13 and 14 June 2018,and compared with observations from the Tropospheric Monitoring Instrument(TROPOMI),Orbiting Carbon Observatory-2(OCO-2),and Copernicus Atmosphere Monitoring Service(CAMS).The column-averaged mixing ratio of CO(XCO)monitored by TROPOMI agreed well with that calculated from the in-situ CO pro le,with a small mean diffierence of 5±1.89 ppb.The column-averaged mixing ratio of CO2(XCO2)monitored by OCO-2 also agreed well with that calculated from the in-situ CO2 profile,with a slight bias of 0.4±0.6 ppm.The OCO-2 a posteriori profile had an obvious bias compared with the in-situ CO2 pro le on 13 June,with an underestimation between 600 hPa and 250 hPa and an overestimation below 600 hPa.CAMS also had the same bias compared with the in-situ CO profile;however,CAMS agreed well with the in-situ CO profile on 14 June in the troposphere because a weak cold vortex passed Xilin Hot on 13 June and disappeared on 14 June.In the stratosphere,there were significant biases in the vertical degradation.The striking agreement between the column-averaged mixing ratios and the diffierences in the vertical distribution suggests new exciting applications for the profile product.

Relationship between LYVE-1, VEGFR-3 and CD44 gene expressions and lymphatic metastasis in gastric cancer

AIM: To investigate the expression levels of lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), vascular endothelial growth factor receptor-3 (VEGFR-3) and CD44 genes and the relationship between their lev- els and clinicopathological parameters in gastric cancer.METHODS: Tissue samples were obtained from 33 patients (8 females) with gastric cancer. mRNA levels of LYVE-1, VEGFR-3 and CD44 in normal and tumor tissues were quantitatively measured using real time polymerase chain reaction. The results were correlated with lymph node metastasis, histological type and differentiation of the tumor, T-stage, and presence of vascular, perineural and lymphatic invasions. The distribution of molecules in the tissue was evaluated using immunohistochemistry. RESULTS: LYVE-1, CD44 and VEGFR-3 gene expression levels were significantly higher in gastric cancer than in normal tissue. While there was no correlation between gene expressions and clinicopathologic fea- tures such as histologic type, differentiation and stage, gene expression levels were found to be increased in conjunction with positive lymph node/total lymph node ratio and the presence of perineural invasion. A significant correlation was also found between LYVE-1 and CD44 over-expressions and perineural invasion and lymph node positivity in gastric cancers. When the dis- tribution of LYVE-1 antibody-stained lymphatic vessels in tissue was evaluated, lymphatic vessels were located intra-tumorally in 13% and peri-tumorally in 27% of the patients. Moreover, lymph node metastases were also positive in all patients with LYVE-1-staining. CONCLUSION: LYVE-1, VEGFR-3 and CD44 all play an important role in lymphangiogenesis, invasion and metastasis. LYVE-1 is a perfectly reliable lymphatic vessel marker and useful for immunohistochemistry.

Relationship of VEGF/VEGFR with immune and cancer cells:staggering or forward?

Vascular endothelial growth factor(VEGF) is primarily known as a proangiogenic factor and is one of the most important growth and survival factors affecting the vascular endothelium. However, recent studies have shown that VEGF also plays a vital role in the immune environment. In addition to the traditional growth factor role of VEGF and VEGF receptors(VEGFRs), they have a complicated relationship with various immune cells. VEGF also reportedly inhibits the differentiation and function of immune cells during hematopoiesis. Dendritic cells(DCs), macrophages, and lymphocytes further express certain types of VEGF receptors.VEGF can be secreted as well by tumor cells through the autocrine pathway and can stimulate the function of cancer stemness.This review will provide a paradigm shift in our understanding of the role of VEGF/VEGFR signaling in the immune and cancer environment.

Effect of total resource allocation effect and economic growth:Chinese experience of 1978-2004

The analytical method of total resource reallocation effect is an evolution of the analytical method of the factors of economic growth. Since the marketization reform in China in 1978, market mechanism has played a more and more important role in resource allocation, and Chinese economy has developed greatly, which is called "the Chinese Miracle". This paper analyzes the economic growth in China from 1978 to 2004 with the analytical method of total resource reallocation effect. The result shows that the annual growth rate of total resource reallocation effect was 0.2%, which was 5.1% of the comprehensive productivity and 0.21% of the gross output growth, i.e. the total resource allocation played a weak role in the economic growth in China. When analyzing it in Chenery's multinational model, we find that Chinese comprehensive productivity growth rate was higher than that in all the income phases of the model, but the total resource allocation effect was obviously lower than that in all the income phases of the model. It indicates that the total resource allocation in China has a great potential, and that to accelerate marketization reform is one of the important issues for Chinese economic development.