4层横向运动板防护长细杆的数值分析

多层横向运动板对垂直来侵长细杆的挤压、剪切能够使长细杆发生挤压和剪切变形，进而降低长细杆后续的侵彻能力，增强装甲的防护效果。利用LS—DYNA软件对多层横向、邻层反向运动的钢装甲板防护钨合金长细杆进行运动板速度和运动板的厚度分配的相关仿真计算。通过对计算结果中开坑形状、后效板侵深和装甲效能进行分析发现，随着板运动速度的增加，后效板开坑深度减小和开坑形状的非对称性加剧，运动板的干扰作用增强及防护效能提高；在运动板总厚度相同的情况下，板的层数越少，防护性能越好。

具有粘弹性本构关系的有限长细杆冲击问题的研究

有限长细杆冲击问题的分析由于引入了粘弹性本构关系而变得比较复杂。针对三参量模型研究了一个端部受冲击载荷作用的有限长细杆的动力学响应问题 。

钻杆挠曲变形对浅海深孔标准贯入试验的影响

分析某填海工程勘察资料中标准贯入试验值差异现象,发现除了钻探工艺、钻杆直径、泥浆浓度、清孔情况、时间效应、钻杆和套管匹配等因素影响SPT击数外,重锤击打时钻杆的多次挠曲或弯曲回弹、船载式平台波动、钻杆振动和晃动、重锤击打频率等也显著影响测试结果,提出了相应的改进和后续研究建议,可提高地质勘察资料的准确性。

套管加固法加固轴压钢管柱力学性能研究

套管加固法加固轴压钢管柱是一种能有效抑制轴压钢管柱屈曲失稳的加固方法,具有理论清晰、构造简单、安全可靠、技术合理、经济适用等显著优点。本文基于ABAQUS软件模拟分析内杆长细比及套管内杆长度比对杆件在轴向压力作用下的破坏机理及极限承载力的影响,选取4种不同套管内杆长度比和6组内杆长细比不同的杆件进行有限元模拟分析,得出套管内杆长度比及内杆长细比对杆件轴压极限承载力的影响规律,为套管加固法在工程中的应用提供一定的设计依据。

Study on Agrobacterium tumefaciens-mediated Transformation of Brassica campestris L. with Fusion Gene Ycoil-bFGF

[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed （ Brassica campestris L. ） by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows： explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.

Endocrine impact of Helicobacter pylori:Focus on ghrelin and ghrelin o-acyltransferase

Ghrelin is predominantly produced by the gastric enteroendocrine cell compartment and is octanoylated by the recently discovered ghrelin o-acyltransferase （GOAT） before secretion into the bloodstream. This octanoylation is essential for many of the biological properties of ghrelin including appetite stimulation and anti-inflammatory properties as only the acylated form of ghrelin binds to the ghrelin receptor, the growth hormone secretagogue receptor （GHS-R）. Given the gastric location of ghrelin production, it is perhaps not surprising that insult to the gastric mucosa affects circulating ghrelin levels in humans. Helicobacter pylori （H. pylon） infects more than fifty percent of the world＇s population and once established within the gastric mucosa, can persist for life. Infection is associated with chronic gastritis, gastric atrophy and ulceration, reduced appetite and a lower body mass index （BMI）. The large majority of studies investigating levels of circulating ghrelin and ghrelin expression in the stomach in patients with H. pylori infection indicate that the bacterium has a negative impact on ghrelin production and/or secretion. Eradication of infection restores ghrelin, improves appetite and increases BMI in some studies, however, a causative relationship between H. pylori-associated serum ghrelin decline and food intake and obesity has not been established. Most studies measure total ghrelin in the circulation although the measurement of the ratio of acyl/total ghrelin gives a clearer indication that the ghrelin acylation process is altered during infection and atrophy. GOAT is essential for the production of biologically-active, acyl ghrelin and the impact of H. pylori on GOAT expression and activity will be highly informative in the future.

A pilot study on using chlorine dioxide gas for disinfection of gastrointestinal endoscopes

Objectives： This pilot study of employing chlorine dioxide （CD） gas to disinfect gastrointestinal endoscopes was conducted to meet the expectations of many endoscopy units in China for a high-efficiency and low-cost disin- fectant. Methods： An experimental prototype with an active circulation mode was designed to use CD gas to disinfect gastrointestinal endoscopes. One type of testing device composed of polytetrafluoroethylene （PTFE） tubes （2 m long, inner diameter 1 mm） and bacterial carrier containers was used to simulate the channel of the endoscope. PTFE bacterial carriers inoculated with Bacillus atrophaeus with or without organic burden were used to evaluate the spor- icidal activity of CD gas. Factors including exposure dosage, relative humidity （RH）, and flow rate （FR） influencing the disinfection effect of CD gas were investigated. Moreover, an autoptic disinfecting test on eight real gastrointestinal endoscopes after clinical use was performed using the experimental prototype. Results： IRH, exposure dosage, or- ganic burden, and the FIR through the channel significantly （P〈0.05） affected the disinfection efficacy of CD gas for a long and narrow lumen. The log reduction increased as FR decreased. Treatment with 4 mg/L CD gas for 30 min at 0.8 L/min FR and 75% IRH, resulted in complete inactivation of spores. Furthermore, all eight endoscopes with a maximum colony-forming unit of 915 were completely disinfected. The cost was only 3 CNY （0.46 USD） for each endoscope. Conclusions： The methods and results reported in this study could provide a basis for further studies on using CD gas for the disinfection of endoscopes.

Generation of nonhuman primate retinitis pigmentosa model by in situ knockout of RHO in rhesus macaque retina

Retinitis pigmentosa(RP)is a form of inherited retinal degenerative diseases that ultimately involves the macula,which is present in primates but not in the rodents.Therefore,creating nonhuman primate(NHP)models of RP is of critical importance to study its mechanism of pathogenesis and to evaluate potential therapeutic options in the future.Here we applied adeno-associated virus(AAV)-delivered CRISPR/SaCas9 technology to knockout the RHO gene in the retinae of the adult rhesus macaque(Macaca mulatta)to investigate the hypothesis whether non-germline mutation of the RHO gene is sufficient to recapitulate RP.Through a series of studies,we were able to demonstrate successful somatic editing of the RHO gene and reduced RHO protein expression.More importantly,the mutant macaque retinae displayed clinical RP phenotypes,including photoreceptor degeneration,retinal thinning,abnormal rod subcellular structures,and reduced photoresponse.Therefore,we suggest somatic editing of the RHO gene is able to phenocopy RP,and the reduced time span in generating NHP mutant accelerates RP research and expands the utility of NHP model for human disease study.