The 185/333 gene family involved in the immune response of sea urchin.One 185/333 c DNA was isolated from Strongylocentrotus intermedius,and named as Si185/333-1.Its full-length c DNA was 1246 bp in length with a 906 ...The 185/333 gene family involved in the immune response of sea urchin.One 185/333 c DNA was isolated from Strongylocentrotus intermedius,and named as Si185/333-1.Its full-length c DNA was 1246 bp in length with a 906 bp open reading frame encoding a protein of 301 aa.The molecular weight of the deduced protein was approximately 33.1 k D with an estimated PI of p H 6.26.Si185/333-1 had high identities(70%–86%) to most of Sp185/333.An extraordinary identity of 92% was found between Si185/333-1 and Sp185/333 C5 alpha(ABR22474).Moderate identities(63%–64%) were displayed between Si185/333-1 and He185/333.Si185/333-1 had similar structure to Sp185/333.A signal-peptide,a gly-rich region and a his-rich region were found in its secondary structure.RGD motif was found in gly-rich region at position 116–118aa.There was no transmembrane region in Si185/333-1.The element pattern of Si185/333-1 is different from any available pattern that identified in Sp185/333.Si185/333-1 clustered together with pattern C Sp185/333 in phylogenetic tree.The Si185/333-1 m RNA could be detected in tissues including peristomial membrane,coelomocytes,muscle of Aristotles lantern,gut and tube feet,with the highest expression level detected in peristomial membrane and a relatively low expression in ovary and testis.The temporal expression of Si185/333-1 in peristomial membrane and coelomocytes were up-regulated after bacterial,β-D-glucan and ds RNA challenges,reaching the maximum at 12 h post-stimulation.The up-regulation was more obvious in coelomocytes,and bacterial challenge triggered the highest response.These results proved that 185/333-1 gene was involved in the immune defense of S.intermedius,while more studies were necessary for its function in S.intermedius immunity.展开更多
The main concern of this paper is to study the influence of the building slenderness ratio on the structural behavior of the residential concrete tall buildings aiming to deepen structure and architect designers under...The main concern of this paper is to study the influence of the building slenderness ratio on the structural behavior of the residential concrete tall buildings aiming to deepen structure and architect designers understanding for such type of buildings. The study is emphasized only on Kuwait city design conditions for wind and seismic loadings. The paper presents an actual case study for adding two thirty stories residential towers with two different slenderness ratios to an existing residential complex. Wind loading is considered using both code values and wind tunnel results. Three dimensional finite element techniques through ETABS software are used in conducting analysis for structures presented here. A serviceability study is performed to ensure that buildings have sufficient stability to limit lateral drift and peak acceleration within the acceptable range of occupancy comfort. In addition, an ultimate strength study is carried out to verify that all the structural elements are designed to withstand factored gravity and lateral loads in a safe manner according to the international building codes. Analysis results are presented and discussed. A brief idea about foundation design of the new towers and its connection to the existing foundation is presented. Finally conclusions are summarized as guidelines for the structural professions of concrete residential tall buildings.展开更多
基金supported by National Natural Science Foundation of China (31402275)National High Technology Research and Development Program of China (863 Program) (2012AA100812)+1 种基金Marine Public Welfare Projects of China (201405003)Supported by Program for Liaoning Excellent Young Scholar in University (LJQ2015016)
文摘The 185/333 gene family involved in the immune response of sea urchin.One 185/333 c DNA was isolated from Strongylocentrotus intermedius,and named as Si185/333-1.Its full-length c DNA was 1246 bp in length with a 906 bp open reading frame encoding a protein of 301 aa.The molecular weight of the deduced protein was approximately 33.1 k D with an estimated PI of p H 6.26.Si185/333-1 had high identities(70%–86%) to most of Sp185/333.An extraordinary identity of 92% was found between Si185/333-1 and Sp185/333 C5 alpha(ABR22474).Moderate identities(63%–64%) were displayed between Si185/333-1 and He185/333.Si185/333-1 had similar structure to Sp185/333.A signal-peptide,a gly-rich region and a his-rich region were found in its secondary structure.RGD motif was found in gly-rich region at position 116–118aa.There was no transmembrane region in Si185/333-1.The element pattern of Si185/333-1 is different from any available pattern that identified in Sp185/333.Si185/333-1 clustered together with pattern C Sp185/333 in phylogenetic tree.The Si185/333-1 m RNA could be detected in tissues including peristomial membrane,coelomocytes,muscle of Aristotles lantern,gut and tube feet,with the highest expression level detected in peristomial membrane and a relatively low expression in ovary and testis.The temporal expression of Si185/333-1 in peristomial membrane and coelomocytes were up-regulated after bacterial,β-D-glucan and ds RNA challenges,reaching the maximum at 12 h post-stimulation.The up-regulation was more obvious in coelomocytes,and bacterial challenge triggered the highest response.These results proved that 185/333-1 gene was involved in the immune defense of S.intermedius,while more studies were necessary for its function in S.intermedius immunity.
文摘The main concern of this paper is to study the influence of the building slenderness ratio on the structural behavior of the residential concrete tall buildings aiming to deepen structure and architect designers understanding for such type of buildings. The study is emphasized only on Kuwait city design conditions for wind and seismic loadings. The paper presents an actual case study for adding two thirty stories residential towers with two different slenderness ratios to an existing residential complex. Wind loading is considered using both code values and wind tunnel results. Three dimensional finite element techniques through ETABS software are used in conducting analysis for structures presented here. A serviceability study is performed to ensure that buildings have sufficient stability to limit lateral drift and peak acceleration within the acceptable range of occupancy comfort. In addition, an ultimate strength study is carried out to verify that all the structural elements are designed to withstand factored gravity and lateral loads in a safe manner according to the international building codes. Analysis results are presented and discussed. A brief idea about foundation design of the new towers and its connection to the existing foundation is presented. Finally conclusions are summarized as guidelines for the structural professions of concrete residential tall buildings.
