The activity and the selectivity of Ru and Pt based carbon catalysts in the selective oxidation of long-chain aliphatic alcohols (C8, C10, C12) have been investigated. Ru/AC and Pt/AC always showed good initial activi...The activity and the selectivity of Ru and Pt based carbon catalysts in the selective oxidation of long-chain aliphatic alcohols (C8, C10, C12) have been investigated. Ru/AC and Pt/AC always showed good initial activity, however deactivation phenomena rapidly depressed the catalytic per-formance of the catalysts. These phenomena can be limited by modification of Ru/AC and Pt/AC with Au improving the durability of the catalyst. Ru/AC and AuRu/AC showed good selectivity to the corresponding aldehyde (>95%) making these catalysts promising for fragrances manufacturing. The advantage in using Au modified catalyst lies on the easier regeneration procedure com-pared to the one necessary for Ru/AC. Pt /AC and AuPt/AC showed a lower selectivity to aldehyde promoting the formation of the acid and the ester formation respectively. The addition of water in the solvent system speeds up the reaction rate but drastically decreased the selectivity to aldehyde especially in the case of Pt based catalysts.展开更多
The cDNA encoding long-chain fatty-acid alcohol oxidase(FAO) was isolated by using RT-PCR method from Candida cloacae grown in a medium containing oleic acid. The cDNA of fatty-acid alcohol oxidase was cloned into exp...The cDNA encoding long-chain fatty-acid alcohol oxidase(FAO) was isolated by using RT-PCR method from Candida cloacae grown in a medium containing oleic acid. The cDNA of fatty-acid alcohol oxidase was cloned into expression plasmid pET17b under T7 promoter and then transformed into E.coli BL21/DE3. The molecular weight of the expressed protein was estimated to be approximately 64 kD by SDS-PAGE. The expressed protein had a specific catalytic activity of fatty-acid alcohol oxidase and its activity was 1090±116 U/ml medium. Northern blotting revealed that there was high expression of fao mRNA in Candida cloacae yeast grown in a medium containing oleic acid.展开更多
长链二醇(LCDs)是一类微藻生物标志物,在海洋沉积物中除了能够指示其生物来源,近年来的工作还表明它们能够很好地用于示踪表层海水温度(SST)和营养盐。本文对冲绳海槽北部OTK-6站位的1根中晚全新世柱状沉积物(长度468 cm)进行了长链二...长链二醇(LCDs)是一类微藻生物标志物,在海洋沉积物中除了能够指示其生物来源,近年来的工作还表明它们能够很好地用于示踪表层海水温度(SST)和营养盐。本文对冲绳海槽北部OTK-6站位的1根中晚全新世柱状沉积物(长度468 cm)进行了长链二醇的提取分析,重建了7 ka B.P.以来的表层海水温度和营养盐变化历史,并与前人的黑潮指标变化进行对比。结果显示,在本研究区基于长链二醇的SST指标可能反映的是年均但略偏暖季的温度,其变化曲线与营养盐指标的变化曲线呈反向变化。而且上述温度和营养盐变化历史与前人给出的黑潮强弱变化历史对应较好,表现出黑潮增强时,SST温度升高,营养盐含量降低,与黑潮水的高温寡营养的特点一致。本文结果显示,在6.0~5.2 ka B.P.、4.2~3.5 ka B.P.、2.5~2.1 ka B.P.、1.8~1.4 ka B.P.以及1.1~0.8 ka B.P.前后黑潮对研究区域的影响加强。因此,认为基于长链二醇的温度和营养盐指标有潜力成为重建古黑潮演变的新型代用指标。展开更多
文摘The activity and the selectivity of Ru and Pt based carbon catalysts in the selective oxidation of long-chain aliphatic alcohols (C8, C10, C12) have been investigated. Ru/AC and Pt/AC always showed good initial activity, however deactivation phenomena rapidly depressed the catalytic per-formance of the catalysts. These phenomena can be limited by modification of Ru/AC and Pt/AC with Au improving the durability of the catalyst. Ru/AC and AuRu/AC showed good selectivity to the corresponding aldehyde (>95%) making these catalysts promising for fragrances manufacturing. The advantage in using Au modified catalyst lies on the easier regeneration procedure com-pared to the one necessary for Ru/AC. Pt /AC and AuPt/AC showed a lower selectivity to aldehyde promoting the formation of the acid and the ester formation respectively. The addition of water in the solvent system speeds up the reaction rate but drastically decreased the selectivity to aldehyde especially in the case of Pt based catalysts.
文摘The cDNA encoding long-chain fatty-acid alcohol oxidase(FAO) was isolated by using RT-PCR method from Candida cloacae grown in a medium containing oleic acid. The cDNA of fatty-acid alcohol oxidase was cloned into expression plasmid pET17b under T7 promoter and then transformed into E.coli BL21/DE3. The molecular weight of the expressed protein was estimated to be approximately 64 kD by SDS-PAGE. The expressed protein had a specific catalytic activity of fatty-acid alcohol oxidase and its activity was 1090±116 U/ml medium. Northern blotting revealed that there was high expression of fao mRNA in Candida cloacae yeast grown in a medium containing oleic acid.
文摘长链二醇(LCDs)是一类微藻生物标志物,在海洋沉积物中除了能够指示其生物来源,近年来的工作还表明它们能够很好地用于示踪表层海水温度(SST)和营养盐。本文对冲绳海槽北部OTK-6站位的1根中晚全新世柱状沉积物(长度468 cm)进行了长链二醇的提取分析,重建了7 ka B.P.以来的表层海水温度和营养盐变化历史,并与前人的黑潮指标变化进行对比。结果显示,在本研究区基于长链二醇的SST指标可能反映的是年均但略偏暖季的温度,其变化曲线与营养盐指标的变化曲线呈反向变化。而且上述温度和营养盐变化历史与前人给出的黑潮强弱变化历史对应较好,表现出黑潮增强时,SST温度升高,营养盐含量降低,与黑潮水的高温寡营养的特点一致。本文结果显示,在6.0~5.2 ka B.P.、4.2~3.5 ka B.P.、2.5~2.1 ka B.P.、1.8~1.4 ka B.P.以及1.1~0.8 ka B.P.前后黑潮对研究区域的影响加强。因此,认为基于长链二醇的温度和营养盐指标有潜力成为重建古黑潮演变的新型代用指标。
