To rapidly detect the harmful algae H.akashiwo qualitatively and quantitatively, sequences of the 18S rDNA deduced from H.akashiwo were used for designing species-specific primers, and a RFQ-PCR (Real-time Fluorescent...To rapidly detect the harmful algae H.akashiwo qualitatively and quantitatively, sequences of the 18S rDNA deduced from H.akashiwo were used for designing species-specific primers, and a RFQ-PCR (Real-time Fluorescent Quantitative Polymerase Chain Reaction) method was developed for quantitative detection of H.akashiwo. Primer H.akashiwo and TaqMan probe were designed, and the specificity of primer was checked with PCR. A calibration curve was constructed with cycle threshold value against visual counted cell number. And the value of the curve was tested with other H.akashiwo samples, which were assayed with both the RFQ-PCR method and visual count under microscope.展开更多
文摘目的探讨优化急诊护理流程对缩短急性缺血性脑卒中患者救治时间应用效果。方法选取2021年3月至8月在北京大兴区中西医结合医院急诊就诊的急性缺血性脑卒中(acute ischemic stroke,AIS)患者110例作为对照组,采用常规护理;同年9月至11月不断优化护理方案;选取2021年12月至2022年10月在大兴区中西医结合医院急诊就诊的AIS患者110例作为治疗组,采用优化后的护理方案。观察比较两组预检分诊——急诊内科医生接诊时间、接诊评估——CT完成时间、预检分诊——溶栓药物首推时间(Door-to-Needle Time,DNT)、静脉溶栓前及溶栓后24小时美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分。结果两组年龄、性别、体重、治疗前NIHSS评分等一般资料基线一致具有可比性,治疗组预检分诊——急诊内科医生接诊时间为4.0(3.0,6.0)分钟,低于对照组的6.0(5.0,8.0)分钟;试验组接诊评估——CT完成时间为22.0(16.0,37.0)分钟,低于对照组的36.0(30.0,45.0)分钟;试验组预检分诊——溶栓药物首推时间为33.0(26.5,50.0)分钟,低于对照组58.0(42.0,73.0)分钟。差异均具有统计学意义(P<0.05)。两组溶栓前后组内、组间NIHSS评分比较,差异均无统计学意义(P>0.05)。结论优化急诊护理流程可以缩短预检分诊——溶栓药物首推时间时间,提高预检分诊——溶栓药物首推时间合格率及溶栓治疗率。
文摘To rapidly detect the harmful algae H.akashiwo qualitatively and quantitatively, sequences of the 18S rDNA deduced from H.akashiwo were used for designing species-specific primers, and a RFQ-PCR (Real-time Fluorescent Quantitative Polymerase Chain Reaction) method was developed for quantitative detection of H.akashiwo. Primer H.akashiwo and TaqMan probe were designed, and the specificity of primer was checked with PCR. A calibration curve was constructed with cycle threshold value against visual counted cell number. And the value of the curve was tested with other H.akashiwo samples, which were assayed with both the RFQ-PCR method and visual count under microscope.
