目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0...目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0009362的表达水平。诱导hBMSCs成骨并检测细胞中circ_0009362、miR-29b-3p的表达。双荧光素酶报告实验检测circ_0009362、miR-29b-3p的相互调控关系。碱性磷酸酶(ALP)试剂盒检测ALP活性,茜素红(ARS)染色法检测钙沉积。结果与健康对照组相比,OP患者的血清外泌体中circ_0009362表达升高,诱导hBMSCs成骨后circ_0009362的表达降低(均P<0.05)。外泌体能降低hBMSCs中ALP活性与钙沉积百分比,该作用通过分泌circ_0009362实现。敲减外泌体中circ_0009362表达能部分抵消外泌体的作用(均P<0.05)。诱导hBMSCs成骨后miR-29b-3p的表达提高(P<0.05)。circ_0009362与miR-29b-3p存在靶向关系,外泌体通过分泌circ_0009362抑制miR-29b-3p的表达。过表达miR-29b-3p促进hBMSCs中ALP活性与钙沉积百分比,该作用被外泌体部分抵消(均P<0.05)。结论OP患者的血清外泌体通过分泌circ_0009362下调miR-29b-3p的表达来抑制hBMSCs成骨分化。展开更多
Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver disease,defined by several phases,ranging from benign fat accumulation to non-alcoholic steatohepatitis(NASH),which can lead to liver cancer and...Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver disease,defined by several phases,ranging from benign fat accumulation to non-alcoholic steatohepatitis(NASH),which can lead to liver cancer and cirrhosis.Although NAFLD is a disease of disordered metabolism,it also involves several immune cell-mediated inflammatory processes,either promoting and/or suppressing hepatocyte inflammation through the secretion of pro-inflammatory and/or anti-inflammatory factors to influence the NAFLD process.However,the underlying disease mechanism and the role of immune cells in NAFLD are still under investigation,leaving many open-ended questions.In this review,we presented the recent concepts about the interplay of immune cells in the onset and pathogenesis of NAFLD.We also highlighted the specific non-immune cells exhibiting immunological properties of therapeutic significance in NAFLD.We hope that this review will help guide the development of future NAFLD therapeutics.展开更多
Adipose-derived mesenchymal stem cells （ADSCs） can be largely and easily obtained from a wide range of sources. Moreover, they have self-renewal ability, multi-differentiation potential, and an important role in imm...Adipose-derived mesenchymal stem cells （ADSCs） can be largely and easily obtained from a wide range of sources. Moreover, they have self-renewal ability, multi-differentiation potential, and an important role in immune regulation. They can secrete a variety of cytokines to regulate the in vivo micro-environment. Therefore, ADSCs are the ideal seed ceils for stem ceils application. This paper reviews the location, isolation, surface markers, proliferation, differentiation and other biological characteristics of ADSCs, as well as their secretory function and relative researches. ADSCs are expected to become excellent seed cells for cell therapy and tissue engineering through in-depth studies.展开更多
文摘目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0009362的表达水平。诱导hBMSCs成骨并检测细胞中circ_0009362、miR-29b-3p的表达。双荧光素酶报告实验检测circ_0009362、miR-29b-3p的相互调控关系。碱性磷酸酶(ALP)试剂盒检测ALP活性,茜素红(ARS)染色法检测钙沉积。结果与健康对照组相比,OP患者的血清外泌体中circ_0009362表达升高,诱导hBMSCs成骨后circ_0009362的表达降低(均P<0.05)。外泌体能降低hBMSCs中ALP活性与钙沉积百分比,该作用通过分泌circ_0009362实现。敲减外泌体中circ_0009362表达能部分抵消外泌体的作用(均P<0.05)。诱导hBMSCs成骨后miR-29b-3p的表达提高(P<0.05)。circ_0009362与miR-29b-3p存在靶向关系,外泌体通过分泌circ_0009362抑制miR-29b-3p的表达。过表达miR-29b-3p促进hBMSCs中ALP活性与钙沉积百分比,该作用被外泌体部分抵消(均P<0.05)。结论OP患者的血清外泌体通过分泌circ_0009362下调miR-29b-3p的表达来抑制hBMSCs成骨分化。
文摘Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver disease,defined by several phases,ranging from benign fat accumulation to non-alcoholic steatohepatitis(NASH),which can lead to liver cancer and cirrhosis.Although NAFLD is a disease of disordered metabolism,it also involves several immune cell-mediated inflammatory processes,either promoting and/or suppressing hepatocyte inflammation through the secretion of pro-inflammatory and/or anti-inflammatory factors to influence the NAFLD process.However,the underlying disease mechanism and the role of immune cells in NAFLD are still under investigation,leaving many open-ended questions.In this review,we presented the recent concepts about the interplay of immune cells in the onset and pathogenesis of NAFLD.We also highlighted the specific non-immune cells exhibiting immunological properties of therapeutic significance in NAFLD.We hope that this review will help guide the development of future NAFLD therapeutics.
基金Supported by The Fund from the Endangered Species Import and Export Management Office of the People’s Republic of China for the Management and Research of Endangered Wild Animals(201441314404)~~
文摘Adipose-derived mesenchymal stem cells （ADSCs） can be largely and easily obtained from a wide range of sources. Moreover, they have self-renewal ability, multi-differentiation potential, and an important role in immune regulation. They can secrete a variety of cytokines to regulate the in vivo micro-environment. Therefore, ADSCs are the ideal seed ceils for stem ceils application. This paper reviews the location, isolation, surface markers, proliferation, differentiation and other biological characteristics of ADSCs, as well as their secretory function and relative researches. ADSCs are expected to become excellent seed cells for cell therapy and tissue engineering through in-depth studies.
