A molecular template synthetic polymer highly selective for m-nisoldipine was prepared by a molecular imprinting technique.The selective binding characteristics of the template polymer were evaluated by Scatchard anal...A molecular template synthetic polymer highly selective for m-nisoldipine was prepared by a molecular imprinting technique.The selective binding characteristics of the template polymer were evaluated by Scatchard analysis and ultraviolet spectrometry.The results showed that the its dissociation constant was estimated to beKd=1.523×10-3mmol/L.The selectivity binding experiment for substrates indicated the polymer gave a high affinity and selectivity for m-nisoldipine.展开更多
文摘目的:采用高效液相色谱法研究2种晶型间尼索地平在大鼠体内的组织分布特征。方法:生物样品在碱性条件下,加入内标尼莫地平,经乙酸乙酯提取后,进行 HPLC 测定。色谱柱为 Diamonsil^(TM)C_(18)柱(250 mm×4.6 mm,5μm);流动相为乙腈-水(62:38),流速1.0 mL·min^(-1);检测波长237 nm。结果:大鼠在口服 A 和 B 两种晶型间尼索地平(30 mg·kg^(-1))后,于10,30,150 min处死,两种晶型在30 min 时组织中浓度较高,150 min 时在除肺外的各脏器中浓度均呈下降趋势。组织匀浆中间尼索地平的线性关系、最低检测限、准确度和精密度均符合分析要求。结论:该法测定大鼠各组织中药物浓度准确、灵敏、可靠,为问尼索地平新药研究与开发提供实验依据。
文摘A molecular template synthetic polymer highly selective for m-nisoldipine was prepared by a molecular imprinting technique.The selective binding characteristics of the template polymer were evaluated by Scatchard analysis and ultraviolet spectrometry.The results showed that the its dissociation constant was estimated to beKd=1.523×10-3mmol/L.The selectivity binding experiment for substrates indicated the polymer gave a high affinity and selectivity for m-nisoldipine.
