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基因Protocadherin-PC对前列腺癌细胞上皮间质化生的影响
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作者 杨学贞 蒋旭 +1 位作者 Tomonori Habuchi 郭应禄 《中华全科医学》 2019年第11期1833-1836,共4页
目的为了分析基因Protocadherin-PC与前列腺癌转移是否有关,研究其对前列腺癌细胞上皮间质化生的影响,从而探索基因Protocadherin-PC与前列腺癌细胞侵袭转移的关系。方法分别采用蛋白免疫印记、形态检测、细胞划痕闭合实验的方法检测通... 目的为了分析基因Protocadherin-PC与前列腺癌转移是否有关,研究其对前列腺癌细胞上皮间质化生的影响,从而探索基因Protocadherin-PC与前列腺癌细胞侵袭转移的关系。方法分别采用蛋白免疫印记、形态检测、细胞划痕闭合实验的方法检测通过Protocadherin-PC siRNA抑制基因Protocadherin-PC表达对雄激素非依赖性前列腺癌细胞DU-145细胞、PC-3细胞上皮间质化生的影响。结果蛋白免疫印记结果提示抑制基因Protocadherin-PC表达可以促进DU-145细胞、PC-3细胞间质上皮化生;形态学检测提示抑制基因Protocadherin-PC表达在形态上可以诱导DU-145细胞、PC-3细胞更接近于雄激素依赖性前列腺癌细胞LNCaP;细胞划痕闭合实验结果提示抑制基因Protocadherin-PC表达可以减慢DU-145细胞、PC-3细胞生长速度。结论通过Protocadherin-PC siRNA抑制基因Protocadherin-PC表达可以降低雄激素非依赖性前列腺癌细胞DU-145、PC-3侵袭转移力,从而揭示基因Protocadherin-PC又一新功能,与晚期前列腺癌转移关系密切。 展开更多
关键词 Protocadherin-PC 前列腺癌 间质上皮化生
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Epithelial-mesenchymal transition and cancermetastasis 被引量:7
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作者 Junjian Deng Ximing Xu 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第3期125-133,共9页
Epithelial-mesenchymal transition (EMT) is initially considered as a physiological phenomenon during the embryogenesis of mammals, as well as a basic biological event maintaining the stability of the vital body. Rec... Epithelial-mesenchymal transition (EMT) is initially considered as a physiological phenomenon during the embryogenesis of mammals, as well as a basic biological event maintaining the stability of the vital body. Recent researches indicated that EMT plays a critical role in various tumors progression, through which epithelial cancers invade and metastasize. The cell characteristics are changed during EMT, in which the cells lose cell-cell and cell-matrix interactions and apical polarity, reorganize their cytoskeleton, and become isolated, motile, as well as resistant to anoikis, then become spindle-shaped mesenchymal cells. This review lays emphasis on studying the cell morphogenesis, makers and molecular mechanism regulation about EMT, discussing the relationship between the EMT and the cancer development and metastasis. 展开更多
关键词 EMT molecular mechanism CANCER METASTASIS
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SMAD7 and SERPINE1 as novel dynamic network biomarkers detect and regulate the tipping point of TGF-beta induced EMT 被引量:6
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作者 Zhonglin Jiang Lina Lu +5 位作者 Yuwei Liu Si Zhang Shuxian Li Guanyu Wang Peng Wang Luonan Chen 《Science Bulletin》 SCIE EI CAS CSCD 2020年第10期842-853,M0004,共13页
Epithelial–mesenchymal transition(EMT) is a complex nonlinear biological process that plays essential roles in fundamental biological processes such as embryogenesis, wounding healing, tissue regeneration,and cancer ... Epithelial–mesenchymal transition(EMT) is a complex nonlinear biological process that plays essential roles in fundamental biological processes such as embryogenesis, wounding healing, tissue regeneration,and cancer metastasis. A hallmark of EMT is the switch-like behavior during state transition, which is characteristic of phase transitions. Hence, detecting the tipping point just before mesenchymal state transition is critical for understanding molecular mechanism of EMT. Through dynamic network biomarkers(DNB) model, a DNB group with 37 genes was identified which can provide the early-warning signals of EMT. Particularly, we found that two DNB genes, i.e., SMAD7 and SERPINE1 promoted EMT by switching their regulatory network which was further validated by biological experiments. Survival analyses revealed that SMAD7 and SERPINE1 as DNB genes further acted as prognostic biomarkers for lung adenocarcinoma. 展开更多
关键词 Dynamic network biomarker Tipping point Epithelial–mesenchymal transition BISTABILITY Quantitative real-time PCR
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