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大鼠三叉神经脊束间质核内接受面口部躯体伤害性信息的calbindinD-28k神经元向孤束核的投射 被引量:6
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作者 马文领 张文斌 《神经解剖学杂志》 CAS CSCD 北大核心 2002年第4期319-323,T065,共6页
为探讨三叉神经脊束间质核内的 calbindin D-2 8k神经元是否接受并传递面口部躯体伤害性信息到孤束核 ,本研究应用荧光金逆行束路追踪结合 FOS和 calbindin D-2 8k免疫荧光组织化学技术 ,观察了三叉神经脊束间质核的 calbindin D-2 8k和... 为探讨三叉神经脊束间质核内的 calbindin D-2 8k神经元是否接受并传递面口部躯体伤害性信息到孤束核 ,本研究应用荧光金逆行束路追踪结合 FOS和 calbindin D-2 8k免疫荧光组织化学技术 ,观察了三叉神经脊束间质核的 calbindin D-2 8k和FOS双重免疫反应阳性的神经元向孤束核的投射。向右侧孤束核内注射荧光金并向右侧上、下唇皮下注射福尔马林 ,发现荧光金逆标细胞和 FOS免疫反应阳性细胞主要分布于注射侧的三叉神经脊束间质核的背侧边缘旁核和三叉旁核 ;大量的 calbindinD-2 8k免疫阳性细胞分布于双侧三叉神经脊束间质核内。此处的大部分荧光金逆标细胞 (约 74.4% )呈 calbindin D-2 8k免疫反应阳性。在此二者的双重阳性细胞中 ,又有一部分 (约 41.0 % )为同时呈 FOS免疫反应阳性的三重阳性神经元。结果提示 ,三叉神经脊束间质核内接受面口部躯体伤害性信息的 calbindin D-2 8k神经元可直接投射至孤束核 ,calbindin D-2 展开更多
关键词 大鼠 三叉神经脊束 间质核 面口部 躯体伤害性信息 CALBINDIN D-28k神经元 孤束 投射 荧光免疫组织化学
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大鼠三叉神经脊束间质核内内脏神经初级传入终末与NOS阳性投射神经元的联系 被引量:1
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作者 熊抗辉 张文斌 +1 位作者 张宇飞 马文领 《解剖学报》 CAS CSCD 北大核心 2002年第2期136-139,共4页
目的 探查间质核 (INV)内内脏传入终末与向臂旁核 (PBN)投射的NOS阳性神经元之间的联系。 方法 逆行、跨神经节追踪以及免疫荧光组织化学方法 ,结合激光共聚焦扫描显微镜观察。 结果 PBN内注射四甲基罗丹明 (TMR)后 ,逆行标记细胞... 目的 探查间质核 (INV)内内脏传入终末与向臂旁核 (PBN)投射的NOS阳性神经元之间的联系。 方法 逆行、跨神经节追踪以及免疫荧光组织化学方法 ,结合激光共聚焦扫描显微镜观察。 结果 PBN内注射四甲基罗丹明 (TMR)后 ,逆行标记细胞主要位于注射侧的INV ,大多属 2 0 μm以下的中、小型细胞。NOS阳性细胞与TMR逆行标记细胞分布区域重叠。NOS TMR双标记细胞分别占NOS阳性细胞总数的 5 4 8% (17 31)和TMR逆行标记细胞总数的 34% (17 4 9)。舌咽和迷走神经内注射生物素化葡聚糖胺 (BDA)跨神经节标记的内脏神经初级传入终末点状膨体贴近双标记细胞胞体 ,呈紧密接触状。 结论 可能存在经INV向PBN投射的内脏伤害性信息传导通路 。 展开更多
关键词 间质核 臂旁 NOS 内脏神经 伤害性传入 INV 动物实验
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大鼠三叉神经脊束间质核接受口面部和内脏伤害性刺激的CB神经元向孤束核的投射
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作者 马文领 张文斌 《解剖学研究》 CAS 2002年第1期73-74,共2页
关键词 三叉神经脊束 间质核 接受口面部 内脏伤害性刺激 CB神经元 孤束 投射
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猫三叉神经脊束间质核的细胞构筑学研究 被引量:3
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作者 杨松 李惠民 李继硕 《神经解剖学杂志》 CAS CSCD 北大核心 1997年第1期59-66,共8页
用Nissl法和Golgi法,对猫三叉神经脊束间质核的位置、形态和细胞构筑进行了研究。确认,此核位于三叉神经脊束核尾侧亚核和极间亚核尾段的外侧、背侧的三叉神经告束内,吻尾长约8mm,主要包括二个部分。(1)在尾侧亚核至极间亚核尾段... 用Nissl法和Golgi法,对猫三叉神经脊束间质核的位置、形态和细胞构筑进行了研究。确认,此核位于三叉神经脊束核尾侧亚核和极间亚核尾段的外侧、背侧的三叉神经告束内,吻尾长约8mm,主要包括二个部分。(1)在尾侧亚核至极间亚核尾段的外侧,细胞集聚呈不连续带状,与内侧的Ⅰ、Ⅱ层间有“蒂”相连;(2)在尾侧亚核吻段和极间亚核尾段的背侧,细胞集聚呈不规则片状,也与I、Ⅱ层相连续,但未形成明显的“蒂”。此核的细胞多为小(直径8~15μm)至中等大小(15~25μm),胞体为圆形,卵圆形或梭形,偶见大细胞(>25μm),呈多角形或三角形.细胞的形态特征与员测亚核Ⅰ、Ⅱ层细胞相似,似为由Ⅰ、Ⅱ层延伸而来者.根据其位置、细胞构筑和纤维联系的全面观察,本文推测此核与口面部伤害性刺激的传递有密切关系。 展开更多
关键词 三叉神经脊束 间质核 细胞构筑 解剖
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低氧培养促进大鼠髓核间质干细胞增殖 被引量:3
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作者 张亮 刘忠军 +4 位作者 王静成 黄泽楠 陈涛 冯新民 陶玉平 《基础医学与临床》 CSCD 2017年第11期1546-1551,共6页
目的研究低氧培养对大鼠髓核间质干细胞(NPMSCs)增殖的影响。方法分离大鼠尾椎髓核获得NPMSCs并进行体外扩增培养,观察细胞形态,流式细胞仪鉴定细胞免疫表型。取第3代NPMSCs分为常氧组和2%低氧组,分别培养7 d及14 d,观察细胞形态;MTT法... 目的研究低氧培养对大鼠髓核间质干细胞(NPMSCs)增殖的影响。方法分离大鼠尾椎髓核获得NPMSCs并进行体外扩增培养,观察细胞形态,流式细胞仪鉴定细胞免疫表型。取第3代NPMSCs分为常氧组和2%低氧组,分别培养7 d及14 d,观察细胞形态;MTT法检测细胞增殖;流式细胞仪检测细胞活力和RT-q PCR检测低氧诱导因子1α(HIF-1α)、葡萄糖转运蛋白1(GLUT-1)、血管内皮生长因子(VEGF)、沉默信息调节蛋白1(SIRT1)及SIRT6的mRNA表达情况。结果原代NPMSCs细胞早期可形成葵花样细胞集落,传代后细胞增殖明显加快,细胞形态以纺锤形为主。第3代细胞高表达干细胞相关阳性表面抗原分子,低表达干细胞相关阴性表面抗原分子。低氧组细胞形态以多角形为主,细胞更容易聚集成团块,且细胞增殖速度明显加快(P<0.05),细胞凋亡率明显下降(P<0.05)。低氧组HIF-1α、VEGF、GLUT-1、SIRT1及SIRT6表达量明显高于常氧组(P<0.05)。结论 2%O2的低氧环境促进髓核间质干细胞增殖,其机制可能与SIRT1及SIRT6介导的HIF-1α信号通路有关。 展开更多
关键词 干细胞 低氧 增殖 缺氧诱导因子-1Α 沉默信息调节蛋白
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白细胞介素1β对大鼠髓核间质干细胞生物学特性的影响 被引量:1
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作者 张宁 关晓明 +3 位作者 马迅 张丽 张辉 宋亮 《中国组织工程研究》 CAS CSCD 2014年第28期4437-4443,共7页
背景:内源性干细胞并未在椎间盘退变的进程发挥修复作用,作者推测可能与相关致病因素的异常作用有关,导致对髓核间质干细胞功能产生一定的抑制效应。目的:观察炎症因子白细胞介素1β对大鼠髓核间质干细胞生物学特性的影响。方法:取3月... 背景:内源性干细胞并未在椎间盘退变的进程发挥修复作用,作者推测可能与相关致病因素的异常作用有关,导致对髓核间质干细胞功能产生一定的抑制效应。目的:观察炎症因子白细胞介素1β对大鼠髓核间质干细胞生物学特性的影响。方法:取3月龄雄性SD大鼠脊柱腰段椎间盘髓核组织,用胶原酶,胰酶序贯消化法分离并培养髓核间质干细胞。采用流式细胞术检测细胞免疫表型CD24,CD34,CD45,CD90,CD105表达;利用RT-PCR检测干细胞基因SOX2,Nanog的表达。观察髓核间质干细胞成脂、成骨、成软骨分化能力。利用流式细胞术检测白细胞介素1β作用于髓核间质干细胞后的凋亡率,荧光定量PCR检测白细胞介素1β作用于髓核间质干细胞后SOX9,蛋白多糖,Ⅱ型胶原酶,caspase-3基因的表达变化。结果与结论:体外分离培养出的细胞表现为纺锤样长梭形;CD90、CD105表达阳性,CD45、CD34、CD24表达阴性,表达干细胞基因SOX2,Nanog,具有成骨和成软骨分化能力,无成脂分化能力。CCK-8检测白细胞介素1β可以抑制髓核间质干细胞的增殖活性,与作用时间、作用浓度存在一定的依赖关系,最佳作用时间为48 h,最佳作用浓度为50μg/L。流式细胞仪检测白细胞介素1β可以使髓核间质干细胞轻度凋亡;荧光定量PCR结果显示:SOX9,蛋白多糖,Ⅱ型胶原的mRNA表达下调,caspase-3表达上调。结果可见白细胞介素1β具有抑制髓核间质干细胞增殖活性并诱导其凋亡的作用,是导致椎间盘自身干细胞无法发挥修复作用的原因之一。 展开更多
关键词 干细胞 骨髓干细胞 干细胞 增殖活性 凋亡 大鼠 山西省自然科学基金
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三叉神经躯体传入成分向孤束核投射的间接途径—HRP跨节和逆行追踪技术的光镜和电镜研究 被引量:20
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作者 张文斌 李继硕 李惠民 《解剖学报》 CAS CSCD 北大核心 1992年第3期253-259,共7页
用HRP跨节及逆行追踪的光镜和电镜技术探索了三叉神经(TGN)躯体性传入成分向孤束核(NTS)的间接投射通路。HRP微量注射于闩平面的NTS后,逆行标记细胞出现于与TGN尾侧亚核背外部邻接的脊束内的间质核。同时,此核又是TGN的舌神经和眶下神... 用HRP跨节及逆行追踪的光镜和电镜技术探索了三叉神经(TGN)躯体性传入成分向孤束核(NTS)的间接投射通路。HRP微量注射于闩平面的NTS后,逆行标记细胞出现于与TGN尾侧亚核背外部邻接的脊束内的间质核。同时,此核又是TGN的舌神经和眶下神经初级传入的投射部位。电镜观察发现,HRP标记的舌神经和眶下神经初级传入终末与间质核内神经元的树突形成较多的突触连接。有部分标记终末与向NTS投射的神经元的逆行标记树突和树突棘在间质核内形成突触连接。另外,本文研究证明,间质核和TGN尾侧亚核浅层的细胞构筑基本相同,且有些间质核的细胞团系由尾侧亚核浅层结构向脊束内突入而形成,故可将两者视为一个统一体。据此,本文作者认为,TGN和NTS之间存在着经间质核中继的两级神经元传入通路,经此通路传入的冲动应属于躯体性质。推测由两级神经元组成的这个通路可能是针刺TGN领域体表穴位调控孤束核内脏机能的结构基础。 展开更多
关键词 孤束 三叉神经支 间质核 HRP
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间质异型核细胞型鼻息肉2例分析及文献复习
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作者 刘筱 江瑞康 《陕西能源职业技术学院学报》 2009年第3期43-44,共2页
通过对新疆煤矿总医院就诊的2例间质异型核细胞型息肉的活检标本进行临床病理分析,提示间质异型核细胞型息肉易被误诊为横纹肌肉瘤和纤维肉瘤等肉瘤,要注意鉴别。鉴别方法是:此型息肉的间质内有大小不等的异型核细胞散在,异型核细... 通过对新疆煤矿总医院就诊的2例间质异型核细胞型息肉的活检标本进行临床病理分析,提示间质异型核细胞型息肉易被误诊为横纹肌肉瘤和纤维肉瘤等肉瘤,要注意鉴别。鉴别方法是:此型息肉的间质内有大小不等的异型核细胞散在,异型核细胞呈梭形,有丰富的嗜酸性胞浆,核大,染色质深,甚至有怪异的巨核细胞,这种细胞表达Vimentin,肌特异性抗体和平滑肌肌动蛋白,此外,还与角蛋白(Keratin)反应,但对结蛋白(desmin)、肌球蛋白(myoglobin),S100,GFAP不反应。透视电镜发现其既有纤维母细胞又有肌细胞的特点。 展开更多
关键词 异型细胞型息肉 鉴别方法 病理分析
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凝视诱发性眼震产生机制及病变部位研究进展 被引量:1
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作者 刘进 冷辉 《中华耳科学杂志》 CSCD 北大核心 2022年第3期481-486,共6页
凝视诱发性眼震(gaze-evoked nystagmus,GEN)是眼球凝视固定偏心位置而诱发的,快相朝向凝视方向的眼震,阳性通常提示中枢病变。本文试述近年来对病理性GEN的产生机制及病变部位的研究,以期帮助在临床上更快地辅助疾病的判断。
关键词 凝视诱发性眼震 舌下前置 前庭内侧 Cajal间质核 小脑小叶
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Progress in Research on Intercellular Movement of Protoplasm in Higher Plants 被引量:3
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作者 张伟成 《Acta Botanica Sinica》 CSCD 2002年第9期1068-1074,共7页
Since nuclear extrusion was rediscovered in young Allium scale by S.H.WU in the years of 1950's systematic investigations on this phenomenon were carried out with various kinds of microscopic techniques a... Since nuclear extrusion was rediscovered in young Allium scale by S.H.WU in the years of 1950's systematic investigations on this phenomenon were carried out with various kinds of microscopic techniques and plant materials to collect more effective evidence to clarify the debate about whether the nuclear extrusion is an artifact or normal event. In the cooperative research of S. H. WU and C. H. LOU the normality of the occurrence of nuclear extrusion either in growing part of plant or in senescent tissue has been confirmed. This event is intimately associated with the physiological state of the tissues/cells and may play an important role in redistribution and reutilization of cell contents. Based on the results obtained a hypothesis of intercellular movement of protoplasm as a means of translocation of organic material in plants was suggested. Chromatin extrusion was also discovered in the pollen mother cells (cytomixis) of certain angiosperms by G. C. ZHENG and his team. Intercellular migration of chromatin appears most frequently at the stage of synizesis. Cytomixis has been studied in relation to variation and evolution. Chromosome aberration has been considered to be closely associated with chromatin extrusion. By vital microscopic observations of the live tissues of garlic (Allium sativum L.) bud and wheat (Triticum aestivum L.) ovule combined with cinemicroscopy and video recording it has been uncovered that, not only the nuclear material but also the cytoplasm could traverse the intercellular channels by vigorous contraction and expansion, and they may simultaneously extrude out of a cell but often asynchronously migrate from one cell to another. The involvement of cytoplasmic constituents in intercellular migration was also detected in pollen mother cells with electron microscopy. Regarding the mechanism of intercellular movement a series of experiments provide convincing evidence showing that this kind of movement is an active metabolic process closely coupled with energy metabolism, and the motive power for driving the extrusion may be supplied by the contractile proteins in protoplasm. 展开更多
关键词 nuclear extrusion PLASMODESMA
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Gastrointestinal stromal tumor causing small bowel intussusception in a patient with Crohn’s disease 被引量:7
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作者 George E Theodoropoulos Dimitrios Linardoutsos +4 位作者 Dimitrios Tsamis Paraskevas Stamopoulos Dimitrios Giannopoulos Flora Zagouri Nikolaos V Michalopoulos 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第41期5224-5227,共4页
We report a case of jejunoileal intussusception in a 42-year-old patient with Crohn's disease caused by a gastrointestinal stromal tumor. The patient complained of vague diffuse abdominal pain for a period of 4 mo... We report a case of jejunoileal intussusception in a 42-year-old patient with Crohn's disease caused by a gastrointestinal stromal tumor. The patient complained of vague diffuse abdominal pain for a period of 4 mo. Intussusception was suspected at computer tomography and magnetic resonance imaging scans. Segmental resection of the small intestine was performed. Pathological examination of the surgical specimen revealed a gastrointestinal stromal tumor as well as aphthous ulcerations and areas of inflammation, which were characteristic of Crohn's disease. This is the f irst report of small bowel intussusception due to a gastrointestinal stromal tumor coexisting with Crohn's disease. 展开更多
关键词 Gastrointestinal stromal tumor Crohn's disease INTUSSUSCEPTION
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Nucleon ~3pF_2 Superfluid Pairing Gap in Asymmetry Nuclear Matter
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作者 MI Ai-Jun ZUO Wei +1 位作者 LI Zeng-Hua Umberto Lombardo 《Communications in Theoretical Physics》 SCIE CAS CSCD 2009年第6期1113-1116,共4页
The 3 P F2 superfluidity of neutron and proton is investigated in isospin-asymmetric nuclear matter within the Brueckner-Hartree-Fock approach and the BCS theory by adopting the Argonne V14 and the Argonne V18 nucleon... The 3 P F2 superfluidity of neutron and proton is investigated in isospin-asymmetric nuclear matter within the Brueckner-Hartree-Fock approach and the BCS theory by adopting the Argonne V14 and the Argonne V18 nucleonnucleon interactions. We find that pairing gaps in the 3PF2 channel predicted by adopting the AV14 interaction are much larger than those by the AV18 interaction. As the isospin-asymmetry increases, the neutron 3 pF2 superfluidity is found to increase rapidly, whereas the proton one turns out to decrease and may even vanish at high enough asymmetries. As a consequence, the neutron 3pF2 superfluidity is much stronger than the proton one at high asymmetries and it predominates over the proton one in dense neutron-rich matter. 展开更多
关键词 3pF2 superfluidity asymmetric nuclear matter Brueckner-Hartree-Fock approach BCS theory
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First result from the Jinping Underground Nuclear Astrophysics experiment JUNA:precise measurement of the 92 keV ^(25)Mg(p,γ)^(26)Al resonance 被引量:14
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作者 Jun Su Hao Zhang +49 位作者 Zhihong Li Paolo Ventura Yunju Li Ertao Li Chen Chen Yangping Shen Gang Lian Bing Guo Xinyue Li Liyong Zhang Jianjun He Yaode Sheng Yinji Chen Luohuan Wang Long Zhang Fuqiang Cao Wei Nan Weike Nan Gexing Li Na Song Baoqun Cui Lihua Chen Ruigang Ma Zhicheng Zhang Taoyu Jiao Bingshui Gao Xiaodong Tang Qi Wu Jiaqing Li Liangting Sun Shuo Wang Shengquan Yan Junhui Liao Youbao Wang Sheng Zeng Ding Nan Qiwen Fan Ningchun Qi Wenliang Sun Xuyuan Guo Peng Zhang Yunhua Chen Yong Zhou Jifang Zhou Jinrong He Changsong Shang Mingchuan Li Jianping Cheng Weiping Liu JUNA Collaboration 《Science Bulletin》 SCIE EI CSCD 2022年第2期125-132,M0003,共9页
The ^(25)Mg(p,γ)^(26)Al reaction plays an important role in the study of cosmic 1.809 MeV γ-ray as a signature of ongoing nucleosynthesis in the Galaxy.At astrophysical temperature around 0.1 GK,the ^(25)Mg(p,γ)^(2... The ^(25)Mg(p,γ)^(26)Al reaction plays an important role in the study of cosmic 1.809 MeV γ-ray as a signature of ongoing nucleosynthesis in the Galaxy.At astrophysical temperature around 0.1 GK,the ^(25)Mg(p,γ)^(26)Al reaction rates are dominated by the 92 keV resonance capture process.We report a precise measurement of the 92 keV ^(25)Mg(p,γ)^(26)Al resonance in the day-one experiment at Jinping Underground Nuclear Astrophysics experiment(JUNA)facility in the China Jinping Underground Laboratory(CJPL).The resonance strength and ground state feeding factor are determined to be 3.8±0.3×10^(-10) eV and 0:660:04,respectively.The results are in agreement with those reported in the previous direct underground measurement within uncertainty,but with significantly reduced uncertainties.Consequently,we recommend new ^(25)Mg(p,γ)^(26)Al reaction rates which are by a factor of 2.4 larger than those adopted in REACLIB database at the temperature around 0.1 GK.The new results indicate higher production rates of ^(26g)Al and the cosmic 1.809 MeV γ-ray.The implication of the new rates for the understanding of other astrophysical situations is also discussed. 展开更多
关键词 Nuclear astrophysics Underground laboratory Cosmic c ray Resonance reaction 26Al
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Weight control in design of space nuclear reactor system 被引量:1
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作者 WANG SanBing HE ChaoHui 《Science China(Technological Sciences)》 SCIE EI CAS 2013年第10期2594-2598,共5页
Space nuclear power system is the key technology for deep space exploration missions in the future, especially for space base building-up. This paper evaluates several typical latest space nuclear reactor system (SNR... Space nuclear power system is the key technology for deep space exploration missions in the future, especially for space base building-up. This paper evaluates several typical latest space nuclear reactor system (SNRS) designs, and finds that most of their weights are heavier than necessary. From the point of weight-control, the SA4 design is the best but its design is more complex than others. A newly designed SNRS, based on the SAFE400 model, uses annular fuel and has better performance, with a fuel mass lower than that of the SAFE400 prototype by 18.75%. Meanwhile, different from former opinions, the delay neutron fractions of SNRS are not constant and change with the different SNRS designs. Therefore, designs of SNRS not to count the delayed neutron fracture or directly to consider it as 0.00677 are not appropriate. 展开更多
关键词 RTG SNRs power density SAFE400 MCNPX
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