AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into thr...AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into three groups:nonsodium deoxycholate(SDOC) group(non-SODC group),SDOC group,and a MSCs intervention group(i.e.,a co-culture system of MSCs and pancreatic acinar cells + SDOC).The cell survival rate,the concentration of malonaldehyde(MDA),the density of superoxide dismutase(SOD),serum amylase(AMS) secretion rate and lactate dehydrogenase(LDH) leakage rate were detected at various time points.In a separate study,Sprague Dawley rats were randomly divided into either an SAP group or an SAP + MSCs group.Serum AMS,MDA and SOD,interleukin(IL)-6,IL-10,and tumor necrosis factor(TNF)-α levels,intestinal mucosa injury scores and proliferating cells of small intestinal mucosa were measured at various time points after injecting either MSCs or saline into rats.In both studies,the protective effect of MSCs was evaluated.RESULTS:In vitro,The cell survival rate of pancreatic acinar cells and the density of SOD were significantly reduced,and the concentration of MDA,AMS secretion rate and LDH leakage rate were significantly increased in the SDOC group compared with the MSCs intervention group and the Non-SDOC group at each time point.In vivo,Serum AMS,IL-6,TNF-α and MAD level in the SAP + MSCs group were lower than the SAP group;however serum IL-10 level was higher than the SAP group.Serum SOD level was higher than the SAP group at each time point,whereas a significant betweengroup difference in SOD level was only noted after 24 h.Intestinal mucosa injury scores was significantly reduced and the proliferating cells of small intestinal mucosa became obvious after injecting MSCs.CONCLUSION:MSCs can effectively relieve injury to pancreatic acinar cells and small intestinal epithelium,promote the proliferation of enteric epithelium and repair of the mucosa,attenuate systemic inflammation in rats with SAP.展开更多
Objective： To observe the effect of moxibustion on the expressions of protein keratinocyte growth factor-1 （KGF-1）, KGF-2, and interleukin-6 （IL-6） in colon of rats with ulcerative colitis （UC）, and to explore ...Objective： To observe the effect of moxibustion on the expressions of protein keratinocyte growth factor-1 （KGF-1）, KGF-2, and interleukin-6 （IL-6） in colon of rats with ulcerative colitis （UC）, and to explore the action mechanism of moxibustion in treating UC. Methods： SD rats were randomized into a normal group, a model group, a herbs-partitioned moxibustion group, and a sulfasalazine （SASP） group. The rats in the herbs-partitioned group were treated with herbs-partitioned moxibustion at Tianshu （ST 25） and Qihai （CV 6）, and those in the SASP group were treated by intragastric administration. After interventions, HE staining and light microscope were adopted in observing the histopathological changes of rat＇s colon, and immunohistochemical methods for detecting the expressions of KGF-1, KGF-2, and IL-6 proteins in rat＇s colon. Results： Compared with the model group, the rats＇ colons in the herbs-partitioned moxibustion group and the SASP group were histopathologically improved; compared with the normal group, the expressions of KGF-1, KGF-2, and IL-6 proteins increased significantly in the model group （P〈0.05）; after intervened by herbs-partitioned moxibustion and SASP respectively, the expressions of KGF-1, KGF-2, and IL-6 proteins in rat＇s colon were decreased markedly （P〈0.05）. Conclusion： Both herbs-partitioned moxibustion and SASP can down-regulate the expressions of KGF-1, KGF-2, and IL-6 proteins in rat＇s colon, which might be one of the mechanisms of herbs-partitioned moxibustion and SASP in treating UC.展开更多
基金Supported by Health and Medicine Scientific Research Foundation of Nanjing Military Area Command,No.08Z029
文摘AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into three groups:nonsodium deoxycholate(SDOC) group(non-SODC group),SDOC group,and a MSCs intervention group(i.e.,a co-culture system of MSCs and pancreatic acinar cells + SDOC).The cell survival rate,the concentration of malonaldehyde(MDA),the density of superoxide dismutase(SOD),serum amylase(AMS) secretion rate and lactate dehydrogenase(LDH) leakage rate were detected at various time points.In a separate study,Sprague Dawley rats were randomly divided into either an SAP group or an SAP + MSCs group.Serum AMS,MDA and SOD,interleukin(IL)-6,IL-10,and tumor necrosis factor(TNF)-α levels,intestinal mucosa injury scores and proliferating cells of small intestinal mucosa were measured at various time points after injecting either MSCs or saline into rats.In both studies,the protective effect of MSCs was evaluated.RESULTS:In vitro,The cell survival rate of pancreatic acinar cells and the density of SOD were significantly reduced,and the concentration of MDA,AMS secretion rate and LDH leakage rate were significantly increased in the SDOC group compared with the MSCs intervention group and the Non-SDOC group at each time point.In vivo,Serum AMS,IL-6,TNF-α and MAD level in the SAP + MSCs group were lower than the SAP group;however serum IL-10 level was higher than the SAP group.Serum SOD level was higher than the SAP group at each time point,whereas a significant betweengroup difference in SOD level was only noted after 24 h.Intestinal mucosa injury scores was significantly reduced and the proliferating cells of small intestinal mucosa became obvious after injecting MSCs.CONCLUSION:MSCs can effectively relieve injury to pancreatic acinar cells and small intestinal epithelium,promote the proliferation of enteric epithelium and repair of the mucosa,attenuate systemic inflammation in rats with SAP.
基金supported by National Basic Research Program of China(973 Program,2009CB522900)Youth Fund Project of the National Natural Science Foundation of China(81001549)+1 种基金Shanghai Program for Cultivation of Elite in Health System(XYQ2011068)2nd Program for Cultivation of Xinglin Scholars by Shanghai University of Traditional Chinese Medicine
文摘Objective： To observe the effect of moxibustion on the expressions of protein keratinocyte growth factor-1 （KGF-1）, KGF-2, and interleukin-6 （IL-6） in colon of rats with ulcerative colitis （UC）, and to explore the action mechanism of moxibustion in treating UC. Methods： SD rats were randomized into a normal group, a model group, a herbs-partitioned moxibustion group, and a sulfasalazine （SASP） group. The rats in the herbs-partitioned group were treated with herbs-partitioned moxibustion at Tianshu （ST 25） and Qihai （CV 6）, and those in the SASP group were treated by intragastric administration. After interventions, HE staining and light microscope were adopted in observing the histopathological changes of rat＇s colon, and immunohistochemical methods for detecting the expressions of KGF-1, KGF-2, and IL-6 proteins in rat＇s colon. Results： Compared with the model group, the rats＇ colons in the herbs-partitioned moxibustion group and the SASP group were histopathologically improved; compared with the normal group, the expressions of KGF-1, KGF-2, and IL-6 proteins increased significantly in the model group （P〈0.05）; after intervened by herbs-partitioned moxibustion and SASP respectively, the expressions of KGF-1, KGF-2, and IL-6 proteins in rat＇s colon were decreased markedly （P〈0.05）. Conclusion： Both herbs-partitioned moxibustion and SASP can down-regulate the expressions of KGF-1, KGF-2, and IL-6 proteins in rat＇s colon, which might be one of the mechanisms of herbs-partitioned moxibustion and SASP in treating UC.
