Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammoni...Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.展开更多
The primary winding of the transformer will generate large inrush current due to the saturation of iron core when an unloaded transformer is switched-on. The inrush current not only causes mechanical interaction force...The primary winding of the transformer will generate large inrush current due to the saturation of iron core when an unloaded transformer is switched-on. The inrush current not only causes mechanical interaction force due to which the windings are damaged, but also induces the differential protection relays to operate incorrectly. In this paper, the mathematical model of unloaded single phase transformer in switch-on is analyzed; the computation formulas of the inrush current and its interruption angle are presented. The experiment investigation of single phase transformers with different capacities shows that the inrush current measurement result is consistent with theoretical analysis. The inrush current waveform is typically a steeple top waveform with high order harmonics and damping in one direction. In the same condition, large inrush current amplitude will be induced with smaller switching angle, larger residual flux and smaller saturation flux.展开更多
文摘Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.
文摘The primary winding of the transformer will generate large inrush current due to the saturation of iron core when an unloaded transformer is switched-on. The inrush current not only causes mechanical interaction force due to which the windings are damaged, but also induces the differential protection relays to operate incorrectly. In this paper, the mathematical model of unloaded single phase transformer in switch-on is analyzed; the computation formulas of the inrush current and its interruption angle are presented. The experiment investigation of single phase transformers with different capacities shows that the inrush current measurement result is consistent with theoretical analysis. The inrush current waveform is typically a steeple top waveform with high order harmonics and damping in one direction. In the same condition, large inrush current amplitude will be induced with smaller switching angle, larger residual flux and smaller saturation flux.