A full length amphioxus cDNA, encoding a novel phosducin-like protein （Amphi-PhLP）, was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open read...A full length amphioxus cDNA, encoding a novel phosducin-like protein （Amphi-PhLP）, was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame （ORF） of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary （CHO） cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.展开更多
The paper regards young adult literature (YAL) as a dissolved-point between localization and globalization of English teaching, and discusses (1) whether the western YAL teaching theories, methods and teaching materia...The paper regards young adult literature (YAL) as a dissolved-point between localization and globalization of English teaching, and discusses (1) whether the western YAL teaching theories, methods and teaching materials accord with Chinese actual situation well or not, (2) if YAL reading is an effective way for creating life-long readers, and (3) why and how to teach YAL. Based on YAL learning and teaching practice, the author uses Soter's YAL theories and alternative approaches, try to clarify three relationships: YA-YAL; adult literature-YAL-children's literature; classics-YAL balance. And put forward three ways for books selection and three reading patterns to YAL teaching.展开更多
基金Supported by the Pilot Projects of Knowledge Innovation Project of Chinese Academy of Sciences (No. KZCX2-YW-211-03 and MGE2008KG06)
文摘A full length amphioxus cDNA, encoding a novel phosducin-like protein （Amphi-PhLP）, was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame （ORF） of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary （CHO） cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.
文摘The paper regards young adult literature (YAL) as a dissolved-point between localization and globalization of English teaching, and discusses (1) whether the western YAL teaching theories, methods and teaching materials accord with Chinese actual situation well or not, (2) if YAL reading is an effective way for creating life-long readers, and (3) why and how to teach YAL. Based on YAL learning and teaching practice, the author uses Soter's YAL theories and alternative approaches, try to clarify three relationships: YA-YAL; adult literature-YAL-children's literature; classics-YAL balance. And put forward three ways for books selection and three reading patterns to YAL teaching.
