We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding s...We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid （SA）, benzothiadiazole, jasmonic acid （JA）, and 1-amino cyclopropane- 1-carboxylic acid, which mediate SA- and JA/ethylene （ET）-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection ofMagnaporthe oryzae, and the induced expression is quicker and stronger during early stages ofpathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense response through SA and/or JA/ET signaling pathways.展开更多
基金supported by the National Natural Science Foundation of China (No. 30771399)the High-Tech Research and Development Program (863) of China (No. 2007AA10Z140)the Research Fund for the Doctoral Program of Higher Education of the Ministry of Education of China (No. 20070335111)
文摘We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid （SA）, benzothiadiazole, jasmonic acid （JA）, and 1-amino cyclopropane- 1-carboxylic acid, which mediate SA- and JA/ethylene （ET）-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection ofMagnaporthe oryzae, and the induced expression is quicker and stronger during early stages ofpathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense response through SA and/or JA/ET signaling pathways.
