使用防腐消毒药应注意以下事项

一、严禁把两种化学性质不同的消毒剂混合使用，如阳离子清洁剂与阴离子清洁剂共同使用时会发生反应，是消毒效果减弱，甚至完全丧失，因此，在重复消毒时，如果用两种化学性质不同的消毒剂，

对“防腐消毒药生石灰”教学内容的异议

笔者讲授《兽医药理学》后,对教材中“防腐消毒药生石灰”的介绍有不同看法,在此,冒昧提出与同行们讨论。1、对生石灰性状的表述教材称“生石灰为白色块状或粉末”,“生石灰几乎不溶于水”,“生石灰水溶性小”等。大家知道,实际上生石灰是白色块状物,吸湿性很强。

一株蜡样芽孢杆菌对小鼠的致病力与抵抗力试验

为了对某益生菌产品中分离到的一株优势生长细菌的特性进行系统研究。利用通过形态学观察、16S rRNA鉴定结果该株细菌为蜡样芽孢杆菌;致病力试验通过以菌浓度1×10~6CFU/mL,按0.1mL/10g腹腔注射小鼠,结果 24h内小鼠无异常表现。从24小时后至第8天,剖杀的小鼠均能从肺、脑等不同的组织器官中分离到该株蜡样芽孢杆菌。药物敏感性试验通过滤纸片扩散法,结果表明该细菌对苯唑西林钠、庆大霉素、土霉素、多西环素、泰乐菌素、替米考星、氟苯尼考、克林霉素、恩诺沙星、诺氟沙星高度敏感,对头孢哌酮钠舒巴坦钠、头孢拉定钠、链霉素、卡那霉素中度敏感,对阿莫西林、氨苄西林、阿米卡星、林可霉素、粘菌素、磺胺嘧啶钠不敏感。细菌对消毒剂耐受性试验采用定性法,试验结果 11种防腐消毒药中仅2%氢氧化钠和3%双氧水对该菌有杀灭作用,其余9种防腐消毒药2%苯酚、2%戊二醛、1%漂白粉、0.5%过氧乙酸、75%酒精、0.1%高锰酸钾、10%鞣酸对该菌无杀灭作用。结论,从益生菌产品中分离获得的这株蜡样芽孢杆菌有一定的致病力,使用该产品有潜在的风险。

Results of gastroscope bacterial decontamination by enzymatic detergent compared to chlorhexidine

AIM： To compare the efficacy of enzymatic detergent with chlorhexidine for gastroscope bacterial decontamination. METHODS： A prospective randomized controlled study was undertaken to evaluate the ability of these 2 agents to achieve high level disinfection in a gastroscope. A total of 260 samples were collected from 5 different gastroscopes. Manual cleaning was done for 10 min with these 2 agents separately （n = 130 each）. Then all specimens underwent 2% glutaraldehyde soaking for 20 min. After 70% alcohol was rinsed, sterile normal saline was flushed into each gastroscope channel and 40 mL of sample was collected. The sample was sent for aerobic bacterial culture after membrane was filtered. A colony count greater than 200 cfu/mL was considered significant. RESULTS： The positive culture rate was 4.6% in the enzymatic detergent arm and 3.1% in the chlorhexidine arm. Pseudomonas species were the main organism detected from both groups （60%）. Multiple organisms were found from 4 specimens （enzymatic detergent arm = 1, chlorhexidine arm = 3）. CONCLUSION： The contamination rate of both types of cleaning solution is equivalent.