魔芋葡甘聚糖对小鼠的防醉解酒作用

目的:以乙醇致醉的醉酒小鼠为对象,研究魔芋葡甘聚糖(KGM)对小鼠的防醉解酒作用。方法:通过解酒、防醉、攀附、检测小鼠全血乙醇含量的实验,探究KGM对小鼠的防醉解酒作用。结果:魔芋葡甘聚糖可显著(p<0.05)延长小鼠对酒精的耐受时间,缩短醉酒的维持时间。在小鼠全血乙醇含量检测中,醉酒小鼠血液乙醇高峰在酒后60min出现,且中、高KGM组较模型组乙醇浓度显著(p<0.05)缩小,因此KGM可明显降低醉酒小鼠血液中乙醇浓度。结论:魔芋葡甘聚糖具有良好的解酒防醉效果。

葛根素及葛根异黄酮对小鼠急性醉酒预防和解酒效果的研究

目的 :研究葛根素及葛根异黄酮对小鼠急性防醉解酒的效果。方法:将小鼠随机分成5组,标记为对照组、葛根粗提物组、葛根异黄酮组、60%葛根素组、99%葛根素组。禁食(不禁水)1 2 h后,每组灌胃等剂量的对应物50%丙二醇水溶液(对照组灌胃50%丙二醇水溶液),30 min后灌胃等剂量56°红星二锅头,观察其醉酒时间与醒酒时间以研究不同含量葛根素对小鼠急性防醉解酒的效果,并在给酒后持续检测小鼠血液中的乙醇含量,以表征不同时段小鼠的醉酒程度。将另一批小鼠随机分为4组,禁食(不禁水)12 h后,分别按100、200、300、400 mg/kg(以体质量计,葛根异黄酮中葛根素含量约为22.13%)剂量灌胃,醉酒时间与醒酒时间以研究葛根素防醉解酒的最佳给药量。结果:随着所给药物中葛根素含量的增高,小鼠急性醉酒的醉酒时间逐渐延长,醒酒时间逐渐缩短。随着葛根素含量的增高,药品解酒防醉的效果越好,但在300 mg/kg(以体质量计)之后,变化趋于缓和。结论:葛根素对小鼠急性防醉解酒有较好的预防效果,葛根中其他异黄酮类物质与葛根素有相似的防醉解酒功效。

Prevention and Cure of Acute Alcohol Intoxication in Mice by Administration of Compound of Japanese Raisintree Fruit, Lobed Kudzuvine Flower Bud and Lightyellow Sophora Root

[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alcohol intoxication in mice. [Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses （low, middle and high） of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde （MDA）, reduced glutathione （GSH） and triglyc-erides （TG） in liver was also determined to investigate the protective effect of drugs on liver. [Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. [Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.