姑息性手术治疗可提高晚期乳房癌病人的生存率

目的 :探讨姑息性手术在晚期乳癌患者综合治疗中的价值。方法 :回顾分析 1993～ 2 0 0 0年间收治的 2 2 7例晚期乳癌的资料 ,并对接受姑息性手术治疗组和未接受手术仅保守治疗组的生存时间和 3年生存率进行对照研究。结果 :晚期乳癌病人接受姑息性手术治疗者平均生存时间和 3年生存率均要高于未接受手术治疗者。而在手术组 ,切缘阴性者生存率大于切缘阳性组。结论 :姑息性手术可能在晚期乳癌患者综合治疗中有一定价值。

不同疗程奥美拉唑治疗儿童消化性溃疡的效果对比

目的比较不同疗程奥美拉唑治疗儿童消化性溃疡的效果。方法选取2016年1月~2017年6月我院收治的60例患儿作为研究对象,根据14碳呼气试验(14C-UBT)检测结果将其分为幽门螺杆菌(Hp)阳性组(40例)和Hp阴性组(20例)。Hp阴性组患儿随机均分为A组(10例)和B组(10例),Hp阳性组患儿随机均分为C组(20例)和D组(20例)。Hp阴性组中,A组患儿口服奥美拉唑2周,B组患儿给予奥美拉唑4周治疗。Hp阳性组中,C组患儿给予奥美拉唑+阿莫西林克拉维酸钾+克拉霉素2周治疗,D组患儿给予奥美拉唑+阿莫西林克拉维酸钾+克拉霉素2周治疗后,停用抗生素,继续予奥美拉唑治疗2周。Hp阳性组和Hp阴性组患儿均在停药4周于我院进行复查胃镜,Hp阳性组患儿同时行14C-UBT检测,分别比较两组患儿的治疗总有效率及药物不良反应发生率,并比较Hp阳性组患儿的14C-UBT。结果 A、B两组患儿的治疗总有效率及药物不良反应发生率比较,差异无统计学意义(P>0.05)。C、D两组患儿的治疗总有效率及药物不良反应发生率比较,差异无统计学意义(P>0.05)。C、D两组患儿的14C-UBT比较,差异无统计学意义(P>0.05)。结论本研究拟通过对消化性溃疡患儿进行疗程2周及4周的综合疗效对比,为临床提供一种治疗儿童消化性溃疡的短疗程方案,增加患儿依从性、减少药物副作用,减轻患儿家属经济负担,具有较好的社会效益及经济效益。

A Rapid and Efficient Method for Preparing Genomic DNA from Microbacterium sp.

A new method was used to preparing genomic DNA from Microbacterium sp.quickly and efficiently.DNA quantity and purity was measured by UV absorbance.Integrity of the genomic DNA was tested by agarose gel eletrophoresis.The DNA prepared by this method was sufficiently pure for PCR.This method saves time and cost,practices easily as well.

Small serotonin-positive pancreatic endocrine tumors caused obstruction of the main pancreatic duct

We report 2 cases of pancreatic endocrine tumors that caused obstruction of the main pancreatic duct(MPD).A 49-year-old asymptomatic man was referred to our institution because dilation of the MPD was revealed by abdominal ultrasonography(US).No tumor was detected by endoscopic ultrasonography,computed tomography(CT),and magnetic resonance imaging(MRI).The diameter of the MPD was > 20 mm at the body,and no dilation was noted at the head.Although malignancy was not confirmed through cytology or imaging,pancreatic cancer was strongly suspected.Pancreaticoduo-denectomy was performed.Pathological and immunohistochemical examination revealed a 5 mm × 3 mm serotonin-positive endocrine tumor.Fibrosis was present around the MPD and seemed to cause stricture.A 32-year-old asymptomatic man had elevated serum amylase,and US demonstrated dilation of the MPD.No tumor was detected by CT and MRI.Pancreatic cancer was suspected due to stricture and dilation of the MPD.Pancreatectomy of middle part of pancreas was performed.Pathological and immunohistochemical examination revealed a serotonin-positive endocrine tumor sized 5 mm × 4 mm.We report 2 cases of serotonin-positive pancreatic endocrine tumors that caused stricture of the MPD in spite of the small size of the tumor.

森林警院学生应对方式特点及其与心理健康的关系

以应对方式问卷、心理健康自评量表为工具,随机选取森林警院320名大学生为被试对象,探讨警院学生应对方式特点及其与心理健康的关系。结果表明:(1)警院学生积极应对方式均分高于消极应对方式均分;遇到应激时,警院学生趋向于选择积极应对方式,而不是消极应对方式。(2)积极应对方式,警院男生得分高于女生,差异不显著;消极应对方式,警院男生得分低于女生,差异不显著。积极应对方式,警院学生正常组高于阳性症状组,差异不显著;消极应对方式,警院学生正常组低于阳性症状组,差异非常显著。(3)警院男生心理健康总分、心理健康各因子与消极应对方式、积极应对方式均有相关。消极应对方式、积极应对方式对警院男生心理健康及各因子有显著预测作用。(4)警院女生躯体化因子、抑郁因子、偏执因子与积极应对方式有显著相关。积极应对方式对警院女生躯体化因子、抑郁因子、偏执因子有显著预测作用。

平板运动试验联合多导联动态心电图对冠心病的诊断价值

目的:研究平板运动试验联合多导联动态心电图对老年人冠心病的诊断意义。方法:选于2017年4月~2019年5月到我院心内科接受治疗的134例疑似冠心病患者,分为TRT组、12LH组、JOINT组,分别对134例疑似冠心病患者进行检测。结果:JOINT组高龄冠心病阳性率(93.26%)均高于TRT组、12LH组、JOINT组阴性率(3.73%)均低于TRT组、12LH组,组间比较有差距,(P<0.05);JOINT组对心肌缺血检出率(94.02%)、心律失常检出率(89.55%)均高于TRT组、12LH组,组间比较有差距,(P<0.05);JOINT组单支病变检测率(60.44%),多支病变检测率(35.82%),均高于TRT组、12LH组,组间比较有差距,(P<0.05);JOINT组ST压低端(85.07%)、MTWA检出率(70.89%)、ST斜率(45.52%)、ST指数(54.47)、ST滞后(76.11)的指标检出率均高于TRT组和12LH组,组间对比有差距(p<0.05);JOINT组敏感度、特异性、ROC分别为80.62%、83.29%、0.831,均高于TRT组和12LH组,组间比较有差距,(P<0.05);结论:平板运动试验联合多导联动态心电图对老年冠心病的诊断准确率高,同时具有经济、无创、安全等诸多优势,对老年冠心病的诊断具有一定的临床指导价值。

HER-2 expression after neoadjuvant chemotherapy of the breast cancers

Objective:The aim of this study was to study changes of HER-2 expression after neoadjuvant chemotherapy in the breast cancer cases.Methods:One hundred and thirty-seven female patients with primary breast cancers,who received neoadjuvant chemotherapy,underwent core needle puncture and Mammotome biopsy before chemotherapy,and the biopsy results were used as the basis of histological diagnosis,fluorescence in situ hybridization (FISH) was performed to test HER2 status of tumor tissues before and after chemotherapy.All patients underwent FEC,TE,or AC neoadjuvant chemotherapy of 2-6 cycles before surgery.Results:Twenty-two patients were positive according to FISH test among 137 preoperative patients,8 patients achieved pathological complete remission after chemotherapy (three HER-2 positive patients and five negative patients),91 patients achieved partial remission,24 patients were stable,and 14 cases were invalid.Twenty-two patients were positive according to FISH test (8 patients with pathological complete remission did not undergo test),and positive patients still expressed positively after chemotherapy before neoadjuvant chemotherapy.Three negative patients were converted to be positive,and changes before and after chemotherapy had no statistical difference (P>0.05).Conclusion:Neoadjuvant chemotherapy makes no influence on patients with HER-2 positive expression,while patients with negative expression can be converted to be positive,but without significant difference.

Epithelioid haemangioendothelioma of the lung:one case report with positive pulmonary hilar lymph nodes

Pulmonary epithelioid hemangioendothelioma (PEH) is a rare tumor that occurs among young women and typically presents as bilateral multiple nodules. In this report, we described an unusual case of PEH presented as a single mass in a 46-year-old man, with 6 positive pulmonary hilar lymph nodes. Histologic findings were compared with conventional PEH, except those features such as round to oval-shaped nodules, hypocellular/cellular peripheral zone, hyaline, myxomatous intercellular stroma and intracellular vacuoles, and we found the patient's pulmonary hilar lymph nodes were metastasis and cholesterol crystal were discovered in the focal infarction areas. Immunohistochemically, the tumor cells were strong positive for CD34, CD31, factor VIII related endothelial antigen and vimentin. The patient died 3 and half months after the surgery without receiving any radiotherapy or chemotherapy. This report may contribute to the data on clinical and histological finding of this rare tumour.

Distribution of Oxytocin Hypothamic-Picuitary-Ovary Axis in Guangxi Local Buffalo

To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochemistry SuperPicTureTM two step method was used to detect the distribution of OT in hypothalamatic-pituitary-ovary axis of five femal Guangxi local buffalo. The test results could provide morphology according to study the OT's synthesis and mechanism of action,and could play reference and directions part in breeding Guangxi local buffalo. The test results display:oxytocin immuno reactive (OT-IR) neuronsw eremainly distributed arcuate nucleus,supraoptic nucleus and paraventricular nucleus,and OT-IR neurons was also found in ventromedial nucleus,ventrolateralis nucleus,suprachiasmaticus nucleus,dorsomedial nucleus,mamillary body,anterior hypothalamic nucleus and so on. The OT immunoactive production was found in pituitary and few OT-IR nerve fibers extended to post pituitary from hypophyseal stalk and medium eminence. In ovaries,OT immunoactive productions were only distributed in germinal epithelium cells,granulosa cells and lutein cells. The OT was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of Guangxi local buffalo. The OT immunoactive neurons were first discovered in every main nucleus of Guangxi local buffalo hypothalamus,especially distributed in arcuate nucleus,supraoptic nucleus and paraventricular nucleus.

Expression of Hpa and CD222 in bladder carcinoma and analysis of clinico-pathologic correlation

Objective: Our study aimed to investigate the relationships between the clinico-pathologic features and the heparanase(Hpa) and CD222 expressions in bladder carcinoma. Methods: The expressions of Hpa and CD222 in 95 bladder carcinoma specimens and 20 paraneoplastic bladder tissues(controls) were assessed using the immunohistochemical staining method. Results: The positive expression rates of Hpa and CD222 in bladder carcinoma were 68.42% and 61.05%, respectively. The positive rate of Hpa was significantly higher in the carcinoma specimens than in the control specimens(P < 0.01). Similarly, the Hpa expression in the invasive bladder carcinoma was significantly higher than that in the non-invasive bladder carcinoma(P < 0.01). A positive correlation was observed between the expressions of Hpa and CD222(P < 0.05). The expressions of Hpa and CD222 were significantly correlated with lymphatic invasion and TNM staging(P < 0.05). The 5-year survival rate was significantly higher in negative expression of the Hpa group than that in the positive expression group(P < 0.05). Compared with the non-co-positive expression group, the 5-year survival rate in the co-positive expression of Hpa and CD222 group was significantly lower(P < 0.05). Conclusion: High Hpa and CD222 expressions in tumor tissues were associated with the occurrence and development of bladder carcinoma. Our results provide helpful information for the further diagnosis and therapy of bladder carcinoma.

Expression and significance of cyclin E in gastric carcinoma

Objective:The aim of the study was to investigate the expression and significance of cyclin E in gastric carcinoma.Methods:We detected the expression of cyclin E in three different pathologic types gastric carcinoma samples by immuno-histochemical staining technique (SP method).Results:In 59 gastric carcinoma samples the positive rate of cyclin E expression in gastric carcinoma was 55.93% (33/59), and it was significantly higher than that of normal gastric mucosa (10.53%, 2/19).The positive rates of cyclin E expression in poor differentiation group and mucoid carcinoma group were 68.75% (11/16) and 66.67% (16/24), respectively, and these were significantly higher than that in well-middle differentiation group (31.58%, 6/19), but there was no significant difference between the fronted two groups (P>0.05).Conclusion:The high expression of cyclin E is associated with the progression of gastric carcinoma and probably related to the behavior of cellular biology.

PCR-based Assay for the Detection of Xanthomonas campestris pv. mangiferaeindicae Causing Bacterial Black Spot in Mango

[Objective] This study aimed to develop a PCR assay for detecting Xanthomonas campestris pv. mangiferaeindicae(Xcm) in culture and in planta. [Method] Primers(Xcm HF and Xcm HR) were designed based on the partial sequence of hrp B gene from xanthomonads to develop a PCR assay for Xcm. Furthermore, specificity and sensitivity of the primer pairs were analyzed in detection of genomic DNA and cell from Xcm. [Result] Amplication was positive only with genomic DNA from positive control ATCC11637 and 12 Xcm strains; no PCR products were amplified with genomic DNA from ten other xanthomonads and seven other bacterial species. The sensitivity of detection was 2.4 pg/μl genomic DNA, and 1.8 × 104CFU/ml cells. The primers also worked well for pathogen detection in direct PCR assays of Xcm colonies grown on liquid medium and in PCR assays of total DNA from leaf, branch and fruit lesions. [Conclusion] A PCR assay was successfully established for rapid detection of Xcm in culture and in planta.

Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival

Objective： To evaluate the relation between argyrophilic nucleolar organizer region （AgNOR）-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer （NSCLC）. Methods： A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 （clone MIB-1） and silver nitrate by immunohistochemical and AgNOR-staining methods. Results： The AgNOR area in MIB-l-positive cells of NSCLC is related to clinicopathological parameters under the TNM （tumor, node, and metastasis） system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological （AgNOR area in MIB-l-positive cells）, and clinicopathological （greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation） parameters are independent prognostic factors of NSCLC.Conclusion： The AgNOR area in MIB- 1-positive cells is related to clinicopathological parameters and survival in NSCLC.

Expression of MEK1 in the colorectal cancer and its clinical implication

Objective:The aim of this study was to investigate the relationship between expression of MEK1 protein in the mitogen-activated protein kinase (MAPK) signaling pathway and liver as well as lymph node metastasis in colorectal cancer patients.Methods:Immunohistochemistry was performed to detect the expression of MEK1 protein in primary cancer,normal colonic mucosa,lymph nodes and liver metastatic foci from 86 colorectal cancer patients.Life table analysis was employed to evaluate the association between MEK1 expression and patients' survival.Results:The positive rate of MEK1 expression in the primary cancer,normal colonic mucosa,metastatic lymph nodes and liver metastatic foci was 52.3%,32.6%,71.4% and 78.3%,respectively.The positive rate of MEK1 expression in the primary cancer,metastatic lymph nodes and liver metastatic foci was significantly higher than that in the normal colonic mucosa (P < 0.01).Furthermore,the positive rate of MEK1 expression in stage III and IV colorectal cancer patients was dramatically higher than that in stage I and II colorectal cancer patients (P < 0.01).The positive rate of MEK1 expression in patients with poorly differentiated adenocarcinoma and mucinous adenocarcinoma was significantly higher than patients with well or moderately differentiated adenocarcinoma (P < 0.01).The 3-year disease-free survival rate was 41.3% in MEK1 positive patients and 73.1% in MEK1 negative patients.The survival rate of MEK1 positive patients was significantly lower than that of MEK1 negative patients (P < 0.05).Conclusion:The increased expression of MEK1 was associated with lymph node metastasis and liver metastasis of colorectal cancer.Therefore,detection of MEK1 expression may have important significance in the evaluation of patients' prognosis.

Expression of proto-oncogene Fra-1 in human neoplastic breast tissues

Objective： Invasion and metastasis are the most significant and intrinsic biological characteristics of cancers, also which are main factors of malignant tumor causing treatment failure and death. Recent studies have found that Fra-1 plays an important role on cell migration, invasion, and maintaining malignant phenotype of transformed cells. But there are few stud- ies about the expression and location of Fra-1 in breast tissues and cells being reported .This study just aims to discuss the expression and location of transcription factor Fra-1 in benign and malignant human breast tissues. Methods： The expression of Fra-1 was investigated by immunohistochemistry in neoplastic breast diseases ranging from benign fibroadenoma to very aggressive undifferentiated carcinoma. The correlations of Fra-1 expression with other indicators of breast carcinoma prog- nosis （ER, PR and ErbB2 receptors） were analyzed. Results： All neoplastic breast tissues, either benign or malignant breast tissues, were nuclear immunoreactive for Fra-l-recognizing antibody. In 85% of benign tumors （17/20）, the immunoreactive for Fra-l-recognizing antibody as exclusively restricted to the nuclei. In three cases （3/20, 15%）, focal unequivocal cytoplas- mic staining was also exhibited. Strong positive nuclear staining for Fra-1 was easily seen in all types of breast carcinomas. However the nucleaflcytoplasmic concomitant immunoreactivity was observed in all types of breast carcinomas. A clear shift in Fra-1 immunoreactivity, from an exclusively nuclear to a simultaneous nuclear and cytoplasmic localization was noticed in 90.2% （37/41） of breast carcinomas. No inverse relationship between Fra-1 and ER and PR protein levels was noticed in malignant tumors. The relative expression level of Fra-1 was not correlated with the expression of ErbB2. Conclusion： The overall expression, pattern and intensity of Fra-1 proteins were correlated with breast oncogenesis. Overexpression of Fra-1, leading to a persistent high cytoplasmic accumulation, may play a role in the process of breast carcinogenesis.

Enhancement of Electrical Performance of c-Si Solar Cells with New Texturization and Diffusion Process

Improving cell efficiency and increasing throughput in solar cell industry efforts were put on uniform texturization and optimum diffusion process. To reduce reflectivity, NaOH/KOH （sodium hydroxide/potassium hydroxide） and IPA （isopropyl alcohol） are widely used in standard alkaline texturization of mono c-Si （crystalline silicon） （〈100〉 crystal orientation） wafers, where IPA promotes formation of uniform pyramidal structure but leads to unstable process. In this work, carbohydrates have been investigated as an additive in etchant solution. Moreover uniform phosphorus diffusion process for B2B （back to back） diffusion （loading two wafers in one single slot of quartz boat） has been investigated with single and multiple temperature plateaus. Impact of pre-oxygen step on phosphorus diffusion is investigated in which number of inactive phosphorus at the PSG （phosphosilicate glass）-Si interface is reduced. A batch of 156 PSQ （pseudo square） mono c-Si solar cells with 18%-18.20% efficiency was fabricated which is -0.3% higher than the standard process. The EL （electroluminescence image）, reff （carrier effective life time）, Voc （open circuit voltage）, Isc （short circuit current）, Pvk （peak power） and r/ （efficiency） have been examined and compared with standard.

HPV、TCT及病理检测结果分析

本文选择妇科手术者,回顾性分析,病理检测结果/宫颈脱落细胞液基细胞学检查(TCT)/高危型人体乳头瘤病毒(HR-HPV)基因检测情况。方法 研究对象,妇科手术患者,我院,例数—200例,2018.02.10/2022.12.30,时间,患者术前均进行TCT、HR-HPV检查,术中病理检测,观察病理结果同HPV、TCT检查结果相关性。结果 (1)病理结果:阴性组(包括:CINⅠ级+炎性改变+正常)占比43.50%;阳性组(包括CINⅡ级+CINⅢ级)占比56.50%。(2)病理结果同HPV、TCT检查结果相关性:阳性组患者HPV16/HPV18/HPV31/HPV58/HPV73/HPV82阳性组率>阴性组患者(值11.991、17.076、16.544、9.819、6.800、6.424),差异显著(P<0.05);阳性组患者/阴性组患者,HPV35/HPV39/HPV45/HPV51/HPV68阳性组率比较(值2.365、1.247、0.241、0.018、2.365),无差异(P>0.05)。阴性组患者NILM(正常范围)检出率>阳性组患者(值187.984),差异显著(P<0.05);阳性组患者ASCUS(未明确诊断意义的不典型鳞状细胞)/LSIL(低度鳞状上皮内病变)/HSIL(高度鳞状上皮内病变)/scc(鳞状细胞癌)检出率>阴性组患者(值25.067、20.998、30.745、18.138),差异显著(P<0.05)。结论 HPV、TCT近年来在宫颈癌筛查领域应用越来越广泛,本次研究比对病理结果,发现不同性质宫颈病变患者HPV检测结果、TCT检测结果差异特征明显,通过两项观察宫颈病变情况具有积极意义,可推广。

Expression of Hsp70 and Caspase-3 in rabbits after se- vere traumatic brain injury

Objective： To investigate the expres- sion of Caspase-3 and Hsp70 in rabbits after severe trau- matic brain injury （TBI） and to explore the feasibility of its application in estimation of injury time in forensic medicine. Methods： Arabbit model of heavy TBI was developed by high velocity impact on the parietal bone with an iron stick. Totally 8 healthy adult New Zealand white rabbits were randomly divided into control group （n=2） and injury group （n=6）. Four hours after injury, tissue specimens from the parietal lobe, temporal lobe, occipital lobe, cerebellum and brainstem were harvested to detect the expression of Hsp70 and Caspase-3 by immunohistochemistry. Besides, the gray values of cells positive for HspT0 and Caspase-3 were analyzed with an image analyzer. Results： Immunohistochemistry staining demonstrated a low level of Caspase-3 and Hsp70 expression in normal control group. While in injury group, both the Caspase-3 and Hsp70 expression was significantly elevated （P〈0.05）. Positive cells gathered around the lesion focus. Occipital lobe and cerebellum had fewer positive cells while temporal and brainstem had the fewest. Conclusion： The expression of Caspase-3 and HspT0 at an early stage following severe TBI is characteristic and can be applied to estimate the time of injury.

Ultra-thin layer structured anodes for highly durable Iow-Pt direct formic acid fuel cells

Direct formic acid fuel cells （DFAFCs） allow highly efficient low temperature conversion of chemical energy into electricity and are expected to play a vital role in our future sustainable society. However, the massive precious metal usage in current membrane electrode assembly （MEA） technology greatly inhibits their actual applications. Here we demonstrate a new type of anode constructed by confining highly active nanoengineered catalysts into an ultra-thin catalyst layer with thickness around 100 nm. Specifically, an atomic layer of platinum is first deposited onto nanoporous gold （NPG） leaf to achieve high utilization of Pt and easy accessibility of both reactants and electrons to active sites. These NPG-Pt core/shell nanostructures are further decorated by a sub-monolayer of Bi to create highly active reaction sites for formic acid electro-oxidation. Thus obtained layer-structured NPG-Pt-Bi thin films allow a dramatic decrease in Pt usage down to 3 ~tg.cm-2, while maintaining very high electrode activity and power performance at sufficiently low overall precious metal loading. Moreover, these electrode materials show superior durability during half-year test in actual DFAFCs, with remarkable resistance to common impurities in formic acid, which together imply their great potential in applications in actual devices.